公开(公告)号：US20070292952A1

公开(公告)日：2007-12-20

申请号：US11587770

申请日：2005-04-15

Applicant: Arun Dhar ,  Thomas Allnutt

Inventor： Arun Dhar ,  Thomas Allnutt

IPC: C12N15/86

CPC classification number: C12N15/85 ,  A01K2227/70 ,  A01K2267/0393 ,  C12N2799/021 ,  C12N2800/90

Abstract: Methods and constructs for genetic manipulation of one or more of shrimp, shellfish, mollusks, and fish are disclosed. The nucleic acid construct includes a promoter and an internal ribosome entry site of an insect picomavirus, such as a cricket paralysis-like picomavirus. One or more open reading frames can be operably associated with one or both of the promoter and the internal ribosome entry site, and one or more proteins or protein subunits can be expressed upon introduction of the construct into a host cell, such as into a shrimp. Method for producing immortalized crustacean cell lines using enhancer elements derived from shrimp and/or shrimp viruses are also described.

Abstract translation: 公开了一种或多种虾，贝类，软体动物和鱼的遗传操作的方法和构造。 核酸构建体包括一种启动子和一种诸如板球麻痹状皮莫瓦病毒之类的皮肤微病毒病毒的内部核糖体进入位点。 一个或多个开放阅读框可以与启动子和内部核糖体进入位点中的一个或两个可操作地相关联，并且一个或多个蛋白质或蛋白质亚基可以在将构建体引入宿主细胞时表达，例如进入虾 。 还描述了使用源自虾和/或虾病毒的增强子元件生产永生化甲壳类细胞系的方法。