公开(公告)号：US20210255075A1

公开(公告)日：2021-08-19

申请号：US16980344

申请日：2019-03-13

Applicant: Amgen Inc.

Inventor： Da REN ,  Melissa SATO

IPC: G01N1/40 ,  G01N33/68 ,  G01N30/06

Abstract: The disclosed methods are directed to preparing polypeptides for multi-attribute analysis. The polypeptides are optionally denatured, reduced, and/or alkylated before being subjected to a first digestion. Following the first digestion the large and small fragments resulting from the digestion are separated from each other. A second digestion is then performed on the larger of the fragments. All of the fragments from the two digestions are then analyzed chromatographically, electrophoretically, or spectrometrically, or a combination of these methods. The methods are especially useful for the preparation of therapeutic polypeptides for analysis, especially those that are not easily cleaved.