公开(公告)号：US10570437B2

公开(公告)日：2020-02-25

申请号：US15323352

申请日：2015-02-27

申请人： Astrego Diagnostics AB

发明人： Johan Elf ,  Ove Öhman ,  George Church

IPC分类号： C12Q1/18 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6806 ,  G01N33/483 ,  B01L3/00 ,  C12Q1/6841

摘要： A library of cell strains is characterized by culturing cells at spatially defined and separated positions in a culturing device (1) and determining a phenotypic characteristic of each cell strain in the culturing device (1). The cells are fixated at the spatially defined and separated positions in the culturing device (1) followed by in situgenotyping a respective variable region (150) of each cell strain at the spatially defined and separated positions in the culturing device (1). Each respective phenotypic characteristic is connected to each respective genotype based on the spatially defined and separated positions in the culturing device (1).

公开(公告)号：US11976316B2

公开(公告)日：2024-05-07

申请号：US16493791

申请日：2018-03-19

申请人： ASTREGO DIAGNOSTICS AB

发明人： Johan Elf ,  Michael Read ,  Özden Baltekin ,  Martin Lovmar ,  Petter Hammar ,  Elias Amselem ,  Mikael Olsson ,  Ove Öhman

IPC分类号： G01N33/50 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/18

CPC分类号： C12Q1/18 ,  G01N33/5008

摘要： Phenotyping of cells involves loading a biological sample into a microfluidic device (1, 100) comprising cell compartments (20, 120) to capture biological material, including target cells, in the sample in the cell compartments (20, 120). A subset (20A) of the cell compartments (20, 120) is identified as comprising target cells exhibiting target phenotype characteristic(s) as determined based on monitoring biological material in the cell compartments (20, 120) prior to addition of a test agent. The biological material is exposed to a test agent and a phenotypic response of the target cells to the test agent is determined based on monitoring target cells in the identified subset (20A) of the cell compartments (20, 120). The phenotyping of the target cells is thereby not overshadowed by the response of other cells and non-cell material present in the biological sample.

公开(公告)号：US11946029B2

公开(公告)日：2024-04-02

申请号：US16969881

申请日：2019-02-15

申请人： ASTREGO DIAGNOSTICS AB

发明人： Özden Baltekin ,  Ove Öhman ,  Martin Lovmar ,  Johan Elf ,  Mikael Olsson

IPC分类号： C12M1/26 ,  B01D61/18 ,  B01D63/00 ,  B01L3/00 ,  C12M3/06

CPC分类号： C12M23/16 ,  B01D61/18 ,  B01D63/005 ,  B01L3/502746 ,  B01L3/502761 ,  C12M33/14 ,  B01L2300/0681

摘要： A microfluidic device (1) comprises a substrate (10) having a flow input channel (30) in fluid connection with a first fluid port (31) and a flow output channel (40) in fluid connection with a third fluid port (41) and cell channels (20) disposed between the flow input channel (30) and the flow output channel (40). The cell channels (20) comprise a respective obstruction (25) designed to prevent the target cells from passing the respective obstruction (25) and into the flow output channel (40). The microfluidic device (1) also comprises a pre-filter (50) with a filter channel (60) in fluid connection with a first filter port (61) and pre-filter channels (70) adapted to accommodate the target cells. A respective first end (72) of the pre-filter channels (70) is in fluid connection with the filter channel (60) and a respective second end (74) of the pre-filter channels (70) is in fluid connection with the flow input channel (30).