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公开(公告)号：US20190382976A1
公开(公告)日：2019-12-19
申请号：US16548522
申请日：2019-08-22
发明人： Edward Kavazanjian , Nasser Hamdan
IPC分类号： E02D3/12 , C04B41/50 , C02F1/42 , C02F1/28 , C02F1/52 , C12N9/78 , B09C1/00 , C01F11/18 , C12N9/80
摘要： The present invention provides methods for mineral precipitation of porous particulate starting materials using isolated urease.
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公开(公告)号：US11987741B2
公开(公告)日：2024-05-21
申请号：US17192337
申请日：2021-03-04
IPC分类号： C09K17/44 , C04B24/14 , C04B28/00 , C04B103/00 , C04B111/00 , E02D3/12
CPC分类号： C09K17/44 , C04B24/14 , C04B28/003 , E02D3/12 , C04B2103/0001 , C04B2111/00732 , C12Y305/01005 , E02D2300/0025 , E02D2300/0045
摘要： The present disclosure provides a method of biocementation comprising contacting a granular, cohesionless soil with a solution, wherein the solution comprises urea, urease, a source of calcium ions, and a source of non-urease proteins, wherein the urea, urease, source of calcium ions, and source of non-urease proteins are provided in effective amounts suitable to cause crystallization of calcium carbonate.
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公开(公告)号：US20180119185A1
公开(公告)日：2018-05-03
申请号：US15803700
申请日：2017-11-03
发明人： Edward Kavazanjian , Nasser Hamdan
CPC分类号： C12P9/00 , C01F11/00 , C04B28/10 , C04B2103/0001 , C04B2111/72 , C12N9/80 , C12P3/00 , C12Y305/01005
摘要： The present invention provides methods for mineral precipitation and/or cementation of permeable or fractured non-porous materials using isolated urease.
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公开(公告)号：US20240263074A1
公开(公告)日：2024-08-08
申请号：US18620705
申请日：2024-03-28
IPC分类号： C09K17/44 , C04B24/14 , C04B28/00 , C04B103/00 , C04B111/00 , E02D3/12
CPC分类号： C09K17/44 , C04B24/14 , C04B28/003 , E02D3/12 , C04B2103/0001 , C04B2111/00732 , C12Y305/01005 , E02D2300/0025 , E02D2300/0045
摘要： The present disclosure provides a method of biocementation comprising contacting a granular, cohesionless soil with a solution, wherein the solution comprises urea, urease, a source of calcium ions, and a source of non-urease proteins, wherein the urea, urease, source of calcium ions, and source of non-urease proteins are provided in effective amounts suitable to cause crystallization of calcium carbonate.
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公开(公告)号：US10392767B2
公开(公告)日：2019-08-27
申请号：US15029316
申请日：2014-10-28
发明人： Edward Kavazanjian , Nasser Hamdan
IPC分类号： E02D3/12 , C04B41/50 , C02F1/42 , C12N9/78 , B09C1/00 , C02F1/28 , C02F1/52 , C01F11/18 , C12N9/80
摘要： The present invention provides methods for mineral precipitation of porous particulate starting materials using isolated urease.
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公开(公告)号：US20210395720A1
公开(公告)日：2021-12-23
申请号：US17411590
申请日：2021-08-25
摘要： Disclosed are methods of extracting an enzyme, comprising soaking a seed in an aqueous solution, homogenizing the seed to produce a homogenized suspension, and filtering the homogenized suspension to produce a crude extract, wherein the crude extract comprises urease and non-urease proteins.
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公开(公告)号：US20210189238A1
公开(公告)日：2021-06-24
申请号：US17192337
申请日：2021-03-04
摘要： The present disclosure provides a method of biocementation comprising contacting a granular, cohesionless soil with a solution, wherein the solution comprises urea, urease, a source of calcium ions, and a source of non-urease proteins, wherein the urea, urease, source of calcium ions, and source of non-urease proteins are provided in effective amounts suitable to cause crystallization of calcium carbonate.
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公开(公告)号：US10399130B2
公开(公告)日：2019-09-03
申请号：US15744358
申请日：2016-07-18
摘要： A method for installing an in situ, low-permeability temporary horizontal barrier at depth for vertical containment of a soil mass including installing a low permeability vertical barrier having walls located to contain a predetermined soil volume, the vertical barrier having a horizontal surface area in a plane perpendicular to its walls; and injecting low permeability reagents capable of forming a temporary horizontal barrier in a pattern suitable for creating a horizontal subsurface barrier joined to all of the vertical barrier walls to form an in situ barrier.
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公开(公告)号：US20190256770A1
公开(公告)日：2019-08-22
申请号：US16077435
申请日：2017-02-13
发明人： Ximin He , Edward Kavazanjian , Nasser Hamdan , Zhi Zhao
摘要： A soil stabilization method of forming an admixture of at least one hydrogel precursor and urease enzyme, and contacting at least a portion of the admixture with soil, and forming a hydrogel network in-situ within at least a portion of the soil, wherein at least a portion of the hydrogel network includes in-situ precipitated calcium carbonate. In some embodiments, at least a portion of the hydrogel network is formed in-situ by polymerizing the at least one hydrogel precursor in the presence of the urease enzyme.
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公开(公告)号：US20180200765A1
公开(公告)日：2018-07-19
申请号：US15744358
申请日：2016-07-18
CPC分类号： B09C1/08 , B09C1/00 , B09C2101/00 , C04B14/104 , C08B37/0033 , C08B37/0096 , E02D31/002 , E02D2250/003 , E02D2600/20
摘要： A method for installing an in situ, low-permeability temporary horizontal barrier at depth for vertical containment of a soil mass including installing a low permeability vertical barrier having walls located to contain a predetermined soil volume, the vertical barrier having a horizontal surface area in a plane perpendicular to its walls; and injecting low permeability reagents capable of forming a temporary horizontal barrier in a pattern suitable for creating a horizontal subsurface barrier joined to all of the vertical barrier walls to form an in situ barrier.
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