公开(公告)号：US20230193322A1

公开(公告)日：2023-06-22

申请号：US17602581

申请日：2020-04-14

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Kylie Standage-Beier ,  Parithi Balachandran ,  Nicholas Brookhouser ,  David Brafman ,  Xiao Wang

IPC: C12N15/90 ,  C12N9/16 ,  C12N9/22 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N9/16 ,  C12Y301/21 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20 ,  C12N2800/80

Abstract: Disclosed are recombinant Cas9 proteins, methods of production, and methods of use for targeted DNA deletions, DNA insertions, or both in a eukaryotic genome. An assay system for evaluating the ability of the recombinant Cas9 proteins for targeted DNA deletions, DNA insertions, or both in a eukaryotic genome is also disclosed.