公开(公告)号：US20180087079A1

公开(公告)日：2018-03-29

申请号：US15828215

申请日：2017-11-30

Applicant: AMGEN INC.

Inventor： Zhimei DU ,  John Douglas MCCARTER ,  Pranhitha REDDY ,  Andrew William SNOWDEN ,  Lawrence R. MCGEE ,  John Gordon ALLEN ,  David Lawrence TREIBER ,  Kathleen KEEGAN ,  Zhihong LI

IPC: C12P21/00 ,  C07D471/04 ,  C07K16/00

CPC classification number: C12P21/00 ,  C07D471/04 ,  C07K16/00

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the addition of cell cycle inhibitors to growing cell cultures.

公开(公告)号：US20150353542A1

公开(公告)日：2015-12-10

申请号：US14760978

申请日：2013-12-11

Applicant: AMGEN INC.

Inventor： Zhimei DU ,  John Douglas MCCARTER ,  Pranhitha REDDY ,  Andrew William SNOWDEN ,  Lawrence R. MCGEE ,  John Gordon ALLEN ,  David Lawrence TREIBER ,  Kathleen KEEGAN ,  Zhihong LI

IPC: C07D471/04

CPC classification number: C12P21/00 ,  C07D471/04 ,  C07K16/00

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the addition of cell cycle inhibitors to growing cell cultures.

Abstract translation: 提供调节表达目的蛋白质的细胞培养物性质的方法。 在各种实施方案中，所述方法涉及将细胞周期抑制剂加入到生长细胞培养物中。

公开(公告)号：US20140199728A1

公开(公告)日：2014-07-17

申请号：US14103418

申请日：2013-12-11

Applicant: Amgen Inc.

Inventor： Zhimei DU ,  John Douglas McCARTER ,  Pranhitha REDDY ,  Andrew William SNOWDEN ,  Lawrence R. MCGEE ,  John Gordon ALLEN ,  David Lawrence TREIBER ,  Kathleen KEEGAN ,  Zhihong LI

IPC: C12P21/00 ,  C07K16/00

CPC classification number: C12P21/00 ,  C07D471/04 ,  C07K16/00

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the addition of cell cycle inhibitors to growing cell cultures.

Abstract translation: 提供调节表达目的蛋白质的细胞培养物性质的方法。 在各种实施方案中，所述方法涉及将细胞周期抑制剂加入到生长细胞培养物中。

公开(公告)号：US20230235024A1

公开(公告)日：2023-07-27

申请号：US17865364

申请日：2022-07-14

Applicant: Amgen Inc.

Inventor： Zhimei DU ,  Pranhitha REDDY ,  Randal B. BASS ,  Feng HE ,  William C. FANSLOW, III ,  Yuling ZHANG ,  Da REN ,  Randall R. KETCHEM

IPC: C07K16/00

CPC classification number: C07K16/00 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/524 ,  C07K2317/14 ,  C07K2319/30

Abstract: The present invention relates to variant Fc-containing molecules, such as antibodies and Fc-fusion molecules, having glycosylation characteristics favorable to large-scale production of therapeutic molecules containing such variant Fc.

公开(公告)号：US20160207980A1

公开(公告)日：2016-07-21

申请号：US14916993

申请日：2014-09-05

Applicant: AMGEN INC.

Inventor： Zhimei DU ,  Pranhitha REDDY ,  Randal B. BASS ,  Feng HE ,  William C. FANSLOW, III ,  Yuling ZHANG ,  Da REN ,  Randal R. KETCHEM

IPC: C07K16/00

CPC classification number: C07K16/00 ,  C07K2317/14 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/524 ,  C07K2319/30

Abstract: The present invention relates to variant Fc-containing molecules, such as antibodies and Fc-fusion molecules, having glycosylation characteristics favorable to largescale production of therapeutic molecules containing such variant Fc. Described herein are compositions and methods to improve glycosylation maturation of and to minimize the culture process-dependent effects of Fc-containing molecules, e.g., Fc-fusion molecules and antibodies. Creating single and multiple amino acid substitutions within the Fc domain with the aim to improve high mannose processing and glycosylation maturation.

Abstract translation: 本发明涉及具有有利于大规模生产含有这种变体Fc的治疗分子的糖基化特征的变体Fc-分子，例如抗体和Fc-融合分子。 本文描述的是改善糖基化成熟并使得含Fc分子例如Fc-融合分子和抗体的培养过程依赖性作用最小化的组合物和方法。 在Fc结构域内产生单个和多个氨基酸取代，目的是改善高甘露糖加工和糖基化成熟。