公开(公告)号：US20240314190A1

公开(公告)日：2024-09-19

申请号：US18577109

申请日：2022-07-20

Applicant: AMGEN INC.

Inventor： Tyler Bellenfant ,  Jeremy S. Conner ,  Sarah Whetstone ,  Thomas Clark Pearson

IPC: H04L65/80 ,  H04N21/236 ,  H04N21/472 ,  H04N23/62 ,  H04N23/661

CPC classification number: H04L65/80 ,  H04N21/236 ,  H04N21/472 ,  H04N23/62 ,  H04N23/661

Abstract: A telepresence system may include at least one telepresence device including a digital camera having camera control inputs and a video feed output. The system may also include at least one remote device having a web browser. The camera control inputs may be communicated from the remote device to the at least one telepresence device via an embedded webserver. The video feed output may be communicated to the remote device via a video server configured to transmit real-time video data from the digital camera, over a secure socket connection, to the remote device.

公开(公告)号：US20240271078A1

公开(公告)日：2024-08-15

申请号：US18567750

申请日：2022-06-06

Applicant: AMGEN INC.

Inventor： Jeremy S. Conner ,  Sarah Whetstone ,  Ketan Kumar

IPC: C12M1/36 ,  C12M1/34

CPC classification number: C12M41/48 ,  C12M41/36 ,  C12M41/46

Abstract: A method of cell culture assessment (e.g., prior to a drug substance harvesting process) includes obtaining a plurality of parameters associated with a cell culture, and inferring or predicting a value or classification indicative of packed cell volume. Inferring or predicting the packed cell volume includes applying the plurality of parameters as inputs to a non-linear machine learning model. The method also includes generating an output indicative of the inferred or predicted value or classification.

公开(公告)号：US20230416667A1

公开(公告)日：2023-12-28

申请号：US18035316

申请日：2021-11-09

Applicant: AMGEN INC.

Inventor： Venkatesh Natarajan ,  Marco DeLiso ,  Jeremy Aaron Bezaire ,  Andrew Cavigli ,  Jeremy S. Conner ,  Jon Hunter ,  Sidney Pehrson ,  Sarah Whetstone

IPC: C12M1/12 ,  C12M1/34 ,  C12M1/00 ,  C12N7/00

CPC classification number: C12M37/00 ,  C12M37/06 ,  C12N7/00 ,  C12M47/10 ,  C12M41/26

Abstract: Automated systems and methods for low-pH viral inactivation include adding an elution pool to a first vessel with an acid. Once first vessel pH probes measure sufficiently low pH, the pool is transferred to a second vessel, where the pH is checked again, and the pool is held for a time sufficient to reduce virus concentration to a safe level, and neutralized, filtered, and transferred to a third vessel. Meanwhile, the first vessel is filled with a known-pH buffer, which is checked against readings from first vessel pH probes to determine whether recalibration is needed. After the pool is transferred to the third vessel, the second vessel is filled with a known—pH buffer, which is checked against readings from second vessel pH probes to determine whether recalibration is needed. The process repeats when the known-pH buffer is dumped and a new elution pool is added to the first vessel.

公开(公告)号：US20230058276A1

公开(公告)日：2023-02-23

申请号：US17794889

申请日：2021-01-22

Applicant: AMGEN INC.

Inventor： Thomas Clark Pearson ,  Sarah Whetstone ,  Jeremy S. Conner

IPC: C07K1/36 ,  C07K1/34 ,  C12M1/26

Abstract: The disclosure provides methods of purifying native and recombinant biomolecules, e.g., proteins, from mammalian cells using purification protocols incorporating harvest recovery operations involving decanter centrifugation of at least one target biomolecule from at least one particulate component of cell culture fluid. The unexpected capacity of decanter centrifuge separation of biological materials of similar densities found in mammalian cell culture fluid has been found to yield high quantities of functional protein in efficient, low-cost harvest recovery steps of biomolecule purification protocols.