公开(公告)号：US11492372B2

公开(公告)日：2022-11-08

申请号：US17554865

申请日：2021-12-17

Applicant: AMGEN INC.

Inventor： Samuel Ray Trejo ,  Robert Perry Brake

IPC: B01D15/36 ,  B01D15/38 ,  C07K1/18 ,  C07K16/00 ,  C07K1/22 ,  C07K14/705 ,  C07K14/715 ,  B01D15/18

Abstract: The invention provides for the removal of a large fraction of contaminants from protein preparations while maintaining a high level of recovery using tentacle anion exchange matrix chromatography medium. Using the methods of the invention, leached affinity chromatography contaminants can be removed from recombinant protein preparations.

公开(公告)号：US11192919B2

公开(公告)日：2021-12-07

申请号：US16042965

申请日：2018-07-23

Applicant: AMGEN INC.

Inventor： Samuel Ray Trejo ,  Robert Perry Brake

IPC: C07K1/18 ,  B01D15/38 ,  C07K16/00 ,  B01D15/36 ,  C07K14/705 ,  C07K14/715 ,  B01D15/18 ,  C07K1/22

Abstract: The invention provides for the removal of a large fraction of contaminants from protein preparations while maintaining a high level of recovery using tentacle anion exchange matrix chromatography medium. Using the methods of the invention, leached affinity chromatography contaminants can be removed from recombinant protein preparations.