公开(公告)号：US11059908B2

公开(公告)日：2021-07-13

申请号：US16338292

申请日：2017-09-28

Applicant: AMGEN INC.

Inventor： Joon Hoi Huh ,  Riki Stevenson ,  Pavel Bondarenko ,  Andrew Nichols ,  Da Ren ,  Neeraj Jagdish Agrawal

IPC: C07K16/40 ,  C07K16/28 ,  G01N11/14

Abstract: The present invention concerns a method for preparing antigen binding proteins with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.

公开(公告)号：US11993663B2

公开(公告)日：2024-05-28

申请号：US17346156

申请日：2021-06-11

Applicant: AMGEN INC.

Inventor： Joon Hoi Huh ,  Riki Stevenson ,  Pavel Bondarenko ,  Andrew Nichols ,  Da Ren ,  Neeraj Jagdish Agrawal ,  Richard Smith

IPC: C07K16/40 ,  C07K16/28 ,  G01N11/14

CPC classification number: C07K16/40 ,  C07K16/2869 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2317/94 ,  G01N11/14

Abstract: The present invention concerns a method for preparing antigen binding proteins specific for PCSK9 with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.