公开(公告)号：US20240270788A1

公开(公告)日：2024-08-15

申请号：US18603661

申请日：2024-03-13

Applicant: AMGEN INC.

Inventor： Rizwan SHARNEZ ,  William TRIEU ,  Marc A. CAPORINI ,  Ron C. Kelly ,  Neill Burt ,  Laura Nicholson ,  Twinkle R. Christian

IPC: C07K1/30 ,  A61K39/395 ,  B01J8/20 ,  B01J8/38 ,  C30B7/00 ,  C30B7/10 ,  C30B30/00 ,  C30B30/02 ,  C30B30/04 ,  G01N24/00 ,  G01N24/08 ,  G01R33/20 ,  G01R33/46

CPC classification number: C07K1/306 ,  A61K39/39591 ,  B01J8/20 ,  B01J8/38 ,  C30B7/00 ,  C30B7/10 ,  C30B30/04 ,  G01N24/087 ,  C30B30/00 ,  C30B30/02 ,  G01N24/00 ,  G01N24/008 ,  G01R33/20 ,  G01R33/46

Abstract: Disclosed herein are methods of preparing a composition comprising crystalline biomolecules, for example, crystalline antibodies. In exemplary embodiments, the method comprises forming a fluidized bed of crystalline biomolecules using, for example, a counter-flow centrifuge to exchange buffer and/or to concentrate the crystalline biomolecules in a solution. Also provided are methods of detecting crystalline biomolecules and/or amorphous biomolecules in a sample.