公开(公告)号：US20130316401A1

公开(公告)日：2013-11-28

申请号：US13939291

申请日：2013-07-11

Applicant: AJINOMOTO CO., INC.

Inventor： Yosuke Nishio ,  Eiichiro Kimura ,  Yoshihiro Usuda ,  Kazuho Ikeo ,  Yoji Nakamura ,  Takashi Gojobori ,  Yutaka Kawarabayashi ,  Yumi Hino ,  Eiichi Hori ,  Jun Yamazaki

IPC: C12N9/88 ,  C12N9/12 ,  C12N9/06

CPC classification number: C12N9/88 ,  C12N9/00 ,  C12N9/0016 ,  C12N9/1205 ,  C12N15/1034 ,  C12N15/1089

Abstract: A method for improving the thermostability of a protein includes introducing, into the protein, two or more amino acid substitutions selected from the group consisting of: (i) substitution of an arginine residue for a lysine residue, (ii) substitution of a threonine residue for a serine residue, and (iii) substitution of an alanine residue for a serine residue.

Abstract translation: 提高蛋白质热稳定性的方法包括向蛋白质中引入选自以下的两个或多个氨基酸取代：（i）精氨酸残基取代赖氨酸残基，（ii）苏氨酸残基的取代 用于丝氨酸残基，和（iii）用丙氨酸残基取代丝氨酸残基。