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公开(公告)号:US20130210006A1
公开(公告)日:2013-08-15
申请号:US13763098
申请日:2013-02-08
申请人: Agrigenetics, Inc.
发明人: Brandon Rapier , Amy Pierce , Jennifer Hamilton , Ana Paula Canu , Juan Pablo Raimondi , Thomas G. Patterson , Nitigna Heath
CPC分类号: C12Q1/24 , C12N15/1003 , C12Q1/686 , C12Q1/6895 , C12Q2600/156
摘要: This disclosure concerns the separation of pericarp tissue from surrounding tissues in grain. Some embodiments concern the isolation of high-purity pericarp DNA from a grain plant that reflects the genotype of the maternal parent of the grain plant, such that the isolated DNA may be used in a PCR-based genotyping assay.
摘要翻译: 本公开涉及从谷物中的周围组织分离果皮组织。 一些实施方案涉及从反映谷物植物的母体亲本的基因型的谷物植物中分离高纯度的果皮DNA,使得分离的DNA可以用于基于PCR的基因分型测定。
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公开(公告)号:US20140170651A1
公开(公告)日:2014-06-19
申请号:US14100713
申请日:2013-12-09
申请人: Agrigenetics, Inc.
发明人: Brandon Rapier , Carol Powers , Christof Stoll
IPC分类号: C12Q1/68
CPC分类号: C12Q1/686 , C12Q1/6806 , C12Q1/6895 , C12Q2563/143 , C12Q2600/13
摘要: This disclosure concerns the isolation of nucleic acids (e.g., genomic DNA) from plant seed material that has been defatted. In some embodiments, such nucleic acids are of sufficient quality and abundance that they may be used in an amplification-based genetic analysis technique; for example and without limitation, to make selections in a plant breeding program.
摘要翻译: 本公开涉及从已经脱脂的植物种子材料中分离核酸(例如基因组DNA)。 在一些实施方案中,这样的核酸具有足够的质量和丰度,可以将它们用于基于扩增的遗传分析技术; 例如但不限于在植物育种计划中进行选择。
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公开(公告)号:US09631232B2
公开(公告)日:2017-04-25
申请号:US14100713
申请日:2013-12-09
申请人: Agrigenetics, Inc
发明人: Brandon Rapier , Carol Powers , Christof Stoll
IPC分类号: C12Q1/68
CPC分类号: C12Q1/686 , C12Q1/6806 , C12Q1/6895 , C12Q2563/143 , C12Q2600/13
摘要: This disclosure concerns the isolation of nucleic acids (e.g., genomic DNA) from plant seed material that has been defatted. In some embodiments, such nucleic acids are of sufficient quality and abundance that they may be used in an amplification-based genetic analysis technique; for example and without limitation, to make selections in a plant breeding program.
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公开(公告)号:US09416394B2
公开(公告)日:2016-08-16
申请号:US13763098
申请日:2013-02-08
申请人: AGRIGENETICS, INC.
发明人: Brandon Rapier , Amy Pierce , Jennifer Hamilton , Ana Paula Canu , Juan Pablo Raimondi , Thomas G. Patterson , Nitigna Heath
CPC分类号: C12Q1/24 , C12N15/1003 , C12Q1/686 , C12Q1/6895 , C12Q2600/156
摘要: Methods for separating pericarp tissue from surrounding tissues in grain are provided. Included are methods for isolation of high-purity pericarp DNA from a grain plant that reflects the genotype of the maternal parent of the grain plant, such that the isolated DNA may be used in a PCR-based genotyping assay.
摘要翻译: 提供了从谷物周围组织分离果皮组织的方法。 包括从反映谷物植物的母本母体的基因型的谷物植物中分离高纯度果皮DNA的方法,使得分离的DNA可用于基于PCR的基因分型测定。
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