公开(公告)号：US20140349335A1

公开(公告)日：2014-11-27

申请号：US14453797

申请日：2014-08-07

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Sailaja CHANDRAPATI ,  Heather M. WEBB ,  Jeffrey C. PEDERSON ,  Kurt J. HALVERSON ,  Brian J. ENGEL

IPC: C12Q1/04

CPC classification number: C12M37/06 ,  C12M23/32 ,  C12M25/06 ,  C12Q1/04 ,  C12Q1/22 ,  G01N21/6486

Abstract: The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent. The second indicator reagent is converted by the predetermined biological activity to a second biological derivative with a second emission spectrum. The first absorbance spectrum includes detectable absorbance in at least a portion of wavelengths present in the second emission spectrum. The first indicator reagent is received and concentrated from an aqueous liquid by a substrate, facilitating the detection of the second biological derivative.

Abstract translation: 本发明提供了检测预定生物活性的方法。 该方法包括使用包含具有第一吸收光谱的第一指示剂和第二指示剂试剂的含水混合物。 第二指示剂通过预定的生物活性转化成具有第二发射光谱的第二生物衍生物。 第一吸收光谱包括在第二发射光谱中存在的波长的至少一部分中的可检测吸光度。 第一指示剂被含水液体接收并通过底物浓缩，便于检测第二生物衍生物。

公开(公告)号：US20200048679A1

公开(公告)日：2020-02-13

申请号：US16492687

申请日：2018-04-02

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Sailaja CHANDRAPATI ,  Alec J. TEAGARDEN ,  Haley A. SADDORIS

IPC: C12Q1/10 ,  C12M1/34 ,  C12Q1/40

Abstract: A device for differentially enumerating colonies of coliform and Escherichia coli microorganisms is provided. The device comprises a water-impermeable first sheet; a water-impermeable second sheet attached to the first sheet; a dry, rehydratable culture medium comprising a lactose-fermentation indicator system, a β-D-glucuronidase indicator system, and a first cold-water soluble gelling agent adhered to the first sheet, the culture medium disposed in a microbial growth zone; and a second cold-water-soluble gelling agent adhered to the second sheet. The microbial growth zone is disposed between the first sheet and the second sheet. The first sheet and second sheet are configured to retard passage of carbon dioxide therethrough. Methods of using the device are also provided.