公开(公告)号：US20110319603A1

公开(公告)日：2011-12-29

申请号：US13255431

申请日：2010-03-10

Applicant: Kazuya Kikuchi ,  Yuichirou Hori ,  Hideki Ueno

Inventor： Kazuya Kikuchi ,  Yuichirou Hori ,  Hideki Ueno

IPC: C07K14/00 ,  C12N15/63 ,  C07C245/04 ,  C07D249/04

CPC classification number: C07K14/215 ,  A61K49/0039 ,  A61K49/0043 ,  A61K49/0045 ,  A61K49/0056 ,  C07D311/82 ,  C07D405/12 ,  C07D405/14 ,  C07K1/13 ,  G01N33/533

Abstract: A method for fluorescently labeling a protein is provided that can also be used for labeling a biomolecule (a protein) in vivo with fluorescence. The method for fluorescently labeling a protein includes obtaining a fusion protein of a target protein to be labeled and, for example, a photoactive yellow protein (PYP), and fluorescently labeling the target protein by reacting the fusion protein with a compound represented by Formula (I), wherein in the compound represented by Formula (I), a coumarin skeleton in Formula (A) or a benzene skeleton in Formula (B) is intramolecnlarly associated with a group X and as a result, fluorescence derived from the group X is suppressed and when in the compound of Formula (I), the coumarin skeleton in Formula (A) or the benzene skeleton in Formula (B) is bound to the PYP or the PYP-derived protein contained in the fusion protein, the coumarin skeleton in Formula (A) or the benzene skeleton in Formula (B) is intramolecnlarly dissociated from the group X, and thereby fluorescence derived from the group X can be emitted.

Abstract translation: 提供了荧光标记蛋白质的方法，其也可用于在体内用荧光标记生物分子（蛋白质）。 荧光标记蛋白质的方法包括获得要标记的靶蛋白质和例如光活性黄蛋白（PYP）的融合蛋白，并通过使融合蛋白与式（ I）表示的化合物，式（I）表示的化合物中，式（A）中的香豆素骨架或式（B）中的苯骨架与基团X进行内分子结合，结果，衍生自基团X的荧光 抑制，当式（I）的化合物中，式（A）中的香豆素骨架或式（B）中的苯骨架与所述融合蛋白中所含的PYP或PYP衍生的蛋白质结合时，所述香豆素骨架在 通式（A）或式（B）中的苯骨架从基团X离子解离，从而可以发射衍生自基团X的荧光。