公开(公告)号：US08283120B2

公开(公告)日：2012-10-09

申请号：US12604640

申请日：2009-10-23

申请人： Yoichi Akagami ,  Masami Kagaya

发明人： Yoichi Akagami ,  Masami Kagaya

IPC分类号： C12Q1/68 ,  G01N33/53 ,  B01F11/00 ,  B28C5/48

CPC分类号： G01N33/5438

摘要： A noncontact stirring method, a noncontact stirring apparatus, a method and apparatus for reacting nucleic acid hybridization using the apparatus, a method for detecting nucleic acid in a sample, an apparatus for detecting nucleic acid, a method for detecting antibodies in a sample, and an apparatus for detecting antibodies in minute droplets 1 μmL or less in an electric field are proposed that can be utilized to speed up biodetection processes, hybridization processes in DNA analysis, ELISA antigen fixing processes, blocking processes, antibody-antigen reaction processes and color reaction processes; and pathogen identification, CRP tests, methods of propagating cells or bacilli such as colon bacilli in a liquid culture medium, and chemical analyses.

摘要翻译： 非接触搅拌方法，非接触搅拌装置，使用该装置的核酸杂交反应的方法和装置，检测样品中的核酸的方法，检测核酸的装置，检测样品中的抗体的方法和 提出了一种用于在电场中检测1μm或更小的微滴中的抗体的装置，其可用于加速生物检测过程，DNA分析中的杂交过程，ELISA抗原定影过程，阻断过程，抗体 - 抗原反应过程和显色反应 过程; 病原体鉴定，CRP检测，细胞培养法或大肠杆菌杆菌在液体培养基中的繁殖方法，化学分析等。

公开(公告)号：US20120003669A1

公开(公告)日：2012-01-05

申请号：US13151730

申请日：2011-06-02

申请人： Yoshihiro MINAMIYA ,  Hiroshi Toda ,  Jun-ichi Ogawa ,  Yoichi Akagami ,  Masami Kagaya

发明人： Yoshihiro MINAMIYA ,  Hiroshi Toda ,  Jun-ichi Ogawa ,  Yoichi Akagami ,  Masami Kagaya

IPC分类号： G01N33/53 ,  C12M1/34 ,  G01N21/64

CPC分类号： G01N33/58 ,  G01N1/30

摘要： An immunohistochemical staining apparatus for carrying out immunohistochemical staining includes a sample chamber (1) in which a primary sample (s1) comprised of a tissue specimen (t) to which a primary antibody (Ig1) is applied is disposed between a sample-side electrode (11) and an opposed electrode (12). The sample chamber (1) has first stirring means for noncontactlessly stirring the primary sample (s1) by applying for a first prescribed time period an alternating electric field adjusted so that the opposed electrode (12) is minus with respect to the sample-side electrode (11), and second stirring means for noncontactlessly stirring a secondary sample (s2) comprised of the noncontactlessly-stirred primary sample (s1) to which a labeled secondary antibody (Ig2) is applied by applying for a second prescribed time period an alternating electric field adjusted so that the opposed electrode (12) is minus with respect to the sample-side electrode (11).

摘要翻译： 用于进行免疫组织化学染色的免疫组织化学染色装置包括：样品室（1），其中将应用了一次抗体（Ig1）的组织标本（t）组成的初级样品（s1）设置在样品侧电极 （11）和相对电极（12）。 样品室（1）具有第一搅拌装置，用于通过施加第一规定时间周期不间断地搅拌初级样品（s1），进行交变电场调整，使得相对电极（12）相对于样品侧电极为负 （11）和第二搅拌装置，用于非接触地搅拌由非接触搅拌的初级样品（s1）组成的二次样品（s2），通过施加第二规定时间段，施加标记的二次抗体（Ig2），交替电 场相对电极（12）相对于取样侧电极（11）为负。

公开(公告)号：US20100173300A1

公开(公告)日：2010-07-08

申请号：US12604640

申请日：2009-10-23

申请人： Yoichi AKAGAMI ,  Masami KAGAYA

发明人： Yoichi AKAGAMI ,  Masami KAGAYA

IPC分类号： B01F13/00 ,  C07H21/00 ,  C12Q1/68 ,  G01N33/566 ,  B01J19/08 ,  C12M1/34

CPC分类号： G01N33/5438

摘要： A noncontact stirring method, a noncontact stirring apparatus, a method and apparatus for reacting nucleic acid hybridization using the apparatus, a method for detecting nucleic acid in a sample, an apparatus for detecting nucleic acid, a method for detecting antibodies in a sample, and an apparatus for detecting antibodies in minute droplets 1 μmL or less in an electric field are proposed that can be utilized to speed up biodetection processes, hybridization processes in DNA analysis, ELISA antigen fixing processes, blocking processes, antibody-antigen reaction processes and color reaction processes; and pathogen identification, CRP tests, methods of propagating cells or bacilli such as colon bacilli in a liquid culture medium, and chemical analyses.

摘要翻译： 非接触搅拌方法，非接触搅拌装置，使用该装置的核酸杂交反应的方法和装置，检测样品中的核酸的方法，检测核酸的装置，检测样品中的抗体的方法和 提出了一种用于在电场中检测1μm或更小的微滴中的抗体的装置，其可用于加速生物检测过程，DNA分析中的杂交过程，ELISA抗原定影过程，阻断过程，抗体 - 抗原反应过程和显色反应 过程; 病原体鉴定，CRP检测，细胞培养法或大肠杆菌杆菌在液体培养基中的繁殖方法，化学分析等。

公开(公告)号：US06857940B2

公开(公告)日：2005-02-22

申请号：US10054937

申请日：2002-01-25

申请人： Yoichi Akagami ,  Yukichi Satou ,  Chikayoshi Yamamoto

发明人： Yoichi Akagami ,  Yukichi Satou ,  Chikayoshi Yamamoto

IPC分类号： B24B57/02 ,  B23H5/08 ,  B24B1/00 ,  B24B37/00 ,  B24B37/04 ,  B24B37/20

CPC分类号： B23H5/08 ,  B24B37/04

摘要： A polishing method with a polishing apparatus includes an electrode constituted as a plurality of electrode elements, a device of driving the electrode, and dielectric abrasive particles disposed between the electrode and a workpiece. Polishing pressure is applied to the particles by a Coulomb force generated when an alternating-current voltage is applied to the electrode.

摘要翻译： 具有抛光装置的抛光方法包括构成多个电极元件的电极，驱动电极的装置和设置在电极和工件之间的电介质磨粒。 抛光压力通过在向电极施加交流电压时产生的库仑力施加到颗粒上。

公开(公告)号：US20050040050A1

公开(公告)日：2005-02-24

申请号：US10959332

申请日：2004-10-07

申请人： Yoichi Akagami ,  Yukichi Satou ,  Chikayoshi Yamamoto

发明人： Yoichi Akagami ,  Yukichi Satou ,  Chikayoshi Yamamoto

IPC分类号： B24B57/02 ,  B23H5/08 ,  B24B1/00 ,  B24B37/00 ,  B24B37/04 ,  B24B37/20 ,  B23H3/00

CPC分类号： B23H5/08 ,  B24B37/04

摘要： A polishing apparatus for polishing a workpiece by utilizing a fluid including abrasive particles having a dielectric property. The polishing apparatus includes an electrode for applying processing pressure to the abrasive particles on the workpiece and having electrode elements for collecting and arranging the abrasive particles by a Coulomb force produced by application of an alternating-current voltage to the electrode, and a driving device for driving the electrode.

摘要翻译： 一种用于通过利用包括具有介电性质的磨料颗粒的流体来抛光工件的抛光装置。 抛光装置包括用于对工件上的磨料颗粒施加处理压力的电极，并具有用于通过向电极施加交流电压产生的库仑力来收集和布置磨料颗粒的电极元件，以及用于 驱动电极。