公开(公告)号：US20120045427A1

公开(公告)日：2012-02-23

申请号：US12747508

申请日：2009-11-17

Applicant: Qirong Shen ,  Yanxia Liu ,  Xingming Yang ,  Yangchun Xu ,  Biao Shen

Inventor： Qirong Shen ,  Yanxia Liu ,  Xingming Yang ,  Yangchun Xu ,  Biao Shen

IPC: A01N63/02 ,  A01P1/00 ,  C12N1/20 ,  C12P39/00

CPC classification number: C05F11/08 ,  A01N63/00 ,  C05G3/02 ,  C12N1/20 ,  C12R1/08 ,  C12R1/085 ,  Y02W30/43

Abstract: The present invention relates to the antagonistic bacteria that are used to prevent and eliminate tobacco bacterial wilt and their microbial organic fertilizer, which belong to the technology for agricultural intensive production. The present invention separates two antagonistic bacteria (Brevibacillus brevis NJL-25 and Bacillus cereus NJL-14) that have remarkable antagonistic action against the pathogens of tobacco bacterial wilt. Microbial organic fertilizer is produced from these antagonistic bacteria and organic compost. Wherein, the content of each of NJL-25 and NJL-14 is above 1×1010 cfu/g, the content of total nitrogen is 4˜5% (above 90% of the nitrogen is organic nitrogen), the content of total nitrogen-phosphorus-kalium nutrient is 6˜10% and the content of organic matter is 30˜35%. As indicated by experiments, after the microbial organic fertilizer is applied into soil, it will enable rapid multiplication of the antagonistic bacteria into a dominant microflora in the soil and achieve more than 97.6% of biocontrol rate of tobacco bacterial wilt in the soil suffering from tobacco bacterial wilt. If the microbial organic fertilizer is applied in the soil without continuous cropping obstacle in a long time, it can prevent the occurrence of the bacterial wilt of continuous cropping tobacco to a great extent.

Abstract translation: 本发明涉及用于预防和消除属于农业密集生产技术的烟草枯萎病及其微生物有机肥的拮抗菌。 本发明分离对烟草细菌枯萎病原体具有显着拮抗作用的两种拮抗菌（Brevibacillus brevis NJL-25和Bacillus cereus NJL-14）。 微生物有机肥由这些拮抗菌和有机堆肥生产。 其中，NJL-25和NJL-14的含量均高于1×10 10 cfu / g，总氮含量为4〜5％（氮有机氮的90％以上），总氮含量 磷钾营养为6〜10％，有机质含量为30〜35％。 如实验所示，微生物有机肥施用于土壤后，能够使拮抗菌快速繁殖，成为土壤中的主要微生物群落，在烟草土壤中达到97.6％以上烟草枯萎病菌生物防治率 细菌枯萎。 如果微生物有机肥在土壤中长时间施用于连续作物的障碍物，可以在很大程度上防止连续作物烟草的枯萎病的发生。

公开(公告)号：US20110214464A1

公开(公告)日：2011-09-08

申请号：US12747510

申请日：2009-11-23

Applicant: Qirong Shen ,  Lihua Chen ,  Xingming Yang ,  Yangchun Xu ,  Wei Ran ,  Qiwei Huang ,  Biao Shen

Inventor： Qirong Shen ,  Lihua Chen ,  Xingming Yang ,  Yangchun Xu ,  Wei Ran ,  Qiwei Huang ,  Biao Shen

IPC: C05F11/08 ,  C12N1/14

CPC classification number: C05F11/08 ,  A01N63/00 ,  A01N63/04 ,  C12N1/14 ,  C12R1/885 ,  Y02W30/43

Abstract: The present invention relates to a biocontrol strain against the wilt of continuous cropping cucumber and watermelon as well as its microbial organic fertilizer, which belongs to the technology for agricultural intensive production. The present invention separates Trichoderma harzianum SQR-T037, which has a remarkable biocontrol effect against wilt of cucumber and watermelon. Microbial organic fertilizer was produced from this biocontrol strain and organic compost. In this fertilizer, the content of the strain is above 1×108 cfu/g, the content of total nitrogen is 3˜5% (above 90% of the nitrogen is organic nitrogen), total nitrogen-phosphorus-kalium nutrient is 6˜10% and organic matter is 30˜35%. As indicated by the experiments, after this microbial organic fertilizer is applied to soil, it will enable rapid multiplication of biocontrol strain into a dominant microflora in the soil and achieve more than 85% of biocontrol rate of wilt in the soil suffering from serious cucumber and watermelon wilt. If this microbial organic fertilizer is applied to the soil without continuous cropping obstacle for a long time, its controlling rate of cucumber and watermelon wilt will be above 80%.

Abstract translation: 本发明涉及连作黄瓜，西瓜枯萎病菌及其微生物有机肥的生物防治菌株，属于农业密集生产技术。 本发明分离了哈茨木霉SQR-T037，对黄瓜和西瓜的枯萎具有显着的生物防治作用。 该生物防治菌株和有机堆肥生产微生物有机肥。 在该肥料中，菌株的含量高于1×10 8 cfu / g，总氮含量为3〜5％（氮有机氮的90％以上），总氮磷钾营养为6〜 10％，有机质为30〜35％。 实验表明，将微生物有机肥施用于土壤后，能够将生物防治菌株快速繁殖为土壤中的主要微生物群落，在黄瓜和黄瓜的土壤中达到85％以上的枯萎病防治率 西瓜枯萎。 如果这种微生物有机肥长期施用于土壤，而不会产生连续的作物障碍，黄瓜和西瓜枯萎病的防治率将达到80％以上。

公开(公告)号：US08476057B2

公开(公告)日：2013-07-02

申请号：US12747508

申请日：2009-11-17

Applicant: Qirong Shen ,  Yanxia Liu ,  Xingming Yang ,  Yangchun Xu ,  Biao Shen

Inventor： Qirong Shen ,  Yanxia Liu ,  Xingming Yang ,  Yangchun Xu ,  Biao Shen

IPC: C12N1/20 ,  A01N63/00

CPC classification number: C05F11/08 ,  A01N63/00 ,  C05G3/02 ,  C12N1/20 ,  C12R1/08 ,  C12R1/085 ,  Y02W30/43

Abstract: The present invention relates to the antagonistic bacteria that are used to prevent and eliminate tobacco bacterial wilt and their microbial organic fertilizer, which belong to the technology for agricultural intensive production. The present invention separates two antagonistic bacteria (Brevibacillus brevis NJL-25 and Bacillus cereus NJL-14) that have remarkable antagonistic action against the pathogens of tobacco bacterial wilt. Microbial organic fertilizer is produced from these antagonistic bacteria and organic compost. Wherein, the content of each of NJL-25 and NJL-14 is above 1×1010 cfu/g, the content of total nitrogen is 4˜5% (above 90% of the nitrogen is organic nitrogen), the content of total nitrogen-phosphorus-kalium nutrient is 6˜10% and the content of organic matter is 30˜35%. As indicated by experiments, after the microbial organic fertilizer is applied into soil, it will enable rapid multiplication of the antagonistic bacteria into a dominant microflora in the soil and achieve more than 97.6% of biocontrol rate of tobacco bacterial wilt in the soil suffering from tobacco bacterial wilt. If the microbial organic fertilizer is applied in the soil without continuous cropping obstacle in a long time, it can prevent the occurrence of the bacterial wilt of continuous cropping tobacco to a great extent.

Abstract translation: 本发明涉及用于预防和消除属于农业密集生产技术的烟草枯萎病及其微生物有机肥的拮抗菌。 本发明分离对烟草细菌枯萎病原体具有显着拮抗作用的两种拮抗菌（Brevibacillus brevis NJL-25和Bacillus cereus NJL-14）。 微生物有机肥由这些拮抗菌和有机堆肥生产。 其中，NJL-25和NJL-14的含量均在1×10 10 cfu / g以上，总氮含量为4〜5％（氮有机氮的90％以上），总氮含量 磷钾营养为6〜10％，有机质含量为30〜35％。 如实验所示，微生物有机肥施用于土壤后，能够使拮抗菌快速繁殖，成为土壤中的主要微生物群落，在烟草土壤中达到97.6％以上烟草枯萎病菌生物防治率 细菌枯萎。 如果微生物有机肥在土壤中长时间施用于连续作物的障碍物，可以在很大程度上防止连续作物烟草的枯萎病的发生。