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    Process for preparation of polypeptides of interest from fusion polypeptides
    1.
    发明授权
    Process for preparation of polypeptides of interest from fusion polypeptides 有权
    从融合多肽制备目的多肽的方法

    公开(公告)号：US07771970B2

    公开(公告)日：2010-08-10

    申请号：US10484478

    申请日：2002-07-26

    Applicant: Woo-Jong Lee ,  Heung-Bok Park ,  Tae-Hoon Cho ,  Jeong-Min Kim ,  Yeon-Sung Park

    Inventor： Woo-Jong Lee ,  Heung-Bok Park ,  Tae-Hoon Cho ,  Jeong-Min Kim ,  Yeon-Sung Park

    IPC: C12P21/00

    CPC classification number: C07K14/00 ,  C07K19/00 ,  C07K2319/00

    Abstract: The present invention relates to a process of separation a protein of interest comprising expressing the protein of interest fused with a fusion partner, selectively adsorbing on matrix, and performing efficient cleavage reaction on the fusion protein adsorbed on matrix or separated from the matrix, and recovering the protein of interest. Also, the present invention provides a fusion protein comprising a protein of interest and a fusion partner, wherein the fusion partner comprises an amino acid sequence which can be cleaved by ubiquitin cleavage enzyme at its C-terminus, and which has a difference in isoelectric point of 1 or more from the protein of interest. According to the present invention, the proteins of interest can be purified in high yield and purity. In addition, complicated separating processes required in the production of recombinant proteins can be eliminated so as to cut down the production cost.

    Abstract translation: 本发明涉及分离感兴趣的蛋白质的方法，包括表达与融合配偶体融合的目的蛋白质，选择性吸附于基质上，并对吸附在基质上或与基质分离的融合蛋白进行有效的切割反应，并回收 感兴趣的蛋白质 此外，本发明提供了包含目标蛋白质和融合配偶体的融合蛋白质，其中融合配偶体包含可在其C末端被泛素切割酶切割的氨基酸序列，并且其等电点 从感兴趣的蛋白质1或更多。 根据本发明，可以高产率和纯度纯化目的蛋白质。 此外，可以消除生产重组蛋白质所需的复杂分离过程，从而降低生产成本。

    Process for preparation of polypeptides of interest from fusion polypeptides
    3.
    发明申请
    Process for preparation of polypeptides of interest from fusion polypeptides 有权
    从融合多肽制备目的多肽的方法

    公开(公告)号：US20050124790A1

    公开(公告)日：2005-06-09

    申请号：US10484478

    申请日：2002-07-26

    Applicant: Woo-Jong Lee ,  Heung-Bok Park ,  Tae-Hoon Cho

    Inventor： Woo-Jong Lee ,  Heung-Bok Park ,  Tae-Hoon Cho

    IPC: C12N15/09 ,  C07K1/14 ,  C07K14/00 ,  C07K19/00 ,  C12P21/02 ,  C07K14/47 ,  C07H21/04 ,  C12P21/04

    CPC classification number: C07K14/00 ,  C07K19/00 ,  C07K2319/00

    Abstract: The present invention relates to a process of separation a protein of interest comprising expressing the protein of interest fused with a fusion partner, selectively adsorbing on matrix, and performing efficient cleavage reaction on the fusion protein adsorbed on matrix or separated from the matrix, and recovering the protein of interest. Also, the present invention provides a fusion protein comprising a protein of interest and a fusion partner, wherein the fusion partner comprises an amino acid sequence which can be cleaved by ubiquitin cleavage enzyme at its C-terminus, and hwich has a difference in isoelectric point of 1 or more from the protein of interest. According to the present invention, the proteins of interest can be purified in high yield and purity. In addition, complicated separating processes required in the production of recombinant proteins can be eliminated so as to cut down the production cost.

    Abstract translation: 本发明涉及分离感兴趣的蛋白质的方法，包括表达与融合配偶体融合的目的蛋白质，选择性吸附于基质上，并对吸附在基质上或与基质分离的融合蛋白进行有效的切割反应，并回收 感兴趣的蛋白质 此外，本发明提供了包含目的蛋白质和融合配偶体的融合蛋白，其中融合配偶体包含可在其C末端被泛素裂解酶切割的氨基酸序列，并且hwich在等电点上具有差异 从感兴趣的蛋白质1或更多。 根据本发明，可以高产率和纯度纯化目的蛋白质。 此外，可以消除生产重组蛋白质所需的复杂分离过程，从而降低生产成本。

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