Method for the transient expression of nucleic acids in plants

    公开(公告)号:US12188029B2

    公开(公告)日:2025-01-07

    申请号:US17557847

    申请日:2021-12-21

    Abstract: Compositions and methods for transiently expressing proteins in a plant are provided. The compositions comprise plants, seeds, plant tissues, and plant parts expressing a protein, wherein the protein is expressed transiently and the transient expression of the protein can be used as a predictive model of how said protein will be expressed in stable transgenic plants in regards to qualitative and quantitative data. The predictive model may be used but is not limited to: promoter evaluation, evaluation of expression cassette construction for best performance (e.g. addition of enhancers or gene silencing suppressors), evaluation of best ways to express heterologous genes.

    Increasing plant transformability by cytotype transfer

    公开(公告)号:US12178173B2

    公开(公告)日:2024-12-31

    申请号:US17600734

    申请日:2020-03-24

    Abstract: A method of altering or transferring the cytotype of a plant line. In particular, transferring the cytotype of a transformation-recalcitrant plant line, e.g., a transformation-recalcitrant maize line, from a transformation-recalcitrant cytotype to a transformable cytotype so that the line becomes transformable while preserving its nuclear genome. The newly-transformable line may be produced using methods including backcrossing and/or haploid induction.

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