公开(公告)号：US4898826A

公开(公告)日：1990-02-06

申请号：US130901

申请日：1987-12-10

申请人： Stephen A. Duffy ,  Christopher P. Prior ,  Randal W. Scott

发明人： Stephen A. Duffy ,  Christopher P. Prior ,  Randal W. Scott

IPC分类号： A61K38/46 ,  A61K38/49 ,  A61K47/12 ,  A61K47/18 ,  C12N9/72

CPC分类号： C12N9/6459 ,  A61K47/12 ,  A61K47/183 ,  A61K9/0019 ,  B01D15/12 ,  C12Y304/21069 ,  A61K38/00 ,  B01D15/34

摘要： Practical amounts of tissue plasminogen activator (tPA) whether secreted by cells naturally producing it or prepared by recombinant means can be solubilized by providing, in aqueous medium at pH 5-14 8 a solubilizing amount of a basic amino acid optionally and preferably, in the presence of a citric acid moiety. The ability to solubilize tPA at concentrations of up to 50 mg is significant as an aid in permitting smaller volumes for purification and permitting single injections of the drug as opposed to intravenous administration.

摘要翻译： 可以通过在pH 5〜14 8的水性介质中提供增溶量的碱性氨基酸，任选地优选在 存在柠檬酸部分。 以高达50mg的浓度溶解tPA的能力是显着的，因为有助于允许较小的体积用于纯化，并允许单次注射药物而不是静脉内施用。

公开(公告)号：US5118796A

公开(公告)日：1992-06-02

申请号：US130827

申请日：1987-12-09

申请人： Christopher P. Prior ,  Stephen A. Duffy ,  Billy J. Moellering

发明人： Christopher P. Prior ,  Stephen A. Duffy ,  Billy J. Moellering

IPC分类号： C07K16/06

CPC分类号： C07K16/065 ,  Y10S530/808 ,  Y10S530/83 ,  Y10S530/864

摘要： Improved methods for direct purification of product immunoglobulins or their derivatives from large volumes of mammalian cell culture medium include directly subjecting the cell culture medium to cation exchange treatment, so as to adsorb the product but not the contaminants. The eluted product is then recycled, or is applied to anion exchange, for further purification, and optionally subjected to additional steps. The product may be obtained in a form suitable for clinical applications, if desired.

摘要翻译： 用于从大量哺乳动物细胞培养基直接纯化产物免疫球蛋白或其衍生物的改进方法包括直接使细胞培养基经受阳离子交换处理，以便吸附产物而不是污染物。 然后将洗脱的产物再循环，或施用于阴离子交换以进一步纯化，并任选地进行另外的步骤。 如果需要，可以以适于临床应用的形式获得产品。