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公开(公告)号：US09714410B2
公开(公告)日：2017-07-25
申请号：US11793784
申请日：2005-12-26
Applicant: Susumu Goto , Shohei Kishishita , Shinya Takuma , Chikashi Hirashima
Inventor： Susumu Goto , Shohei Kishishita , Shinya Takuma , Chikashi Hirashima
CPC classification number: C12N5/0037 , C12N2500/80 , C12N2510/02
Abstract: It is an object of the present invention to allow a cell to produce a protein at a high level using a medium containing an enzymatic degradation product of fish meat or a fish meat extract. A method of culturing a cell comprising starting culturing in an initial medium and feeding at least once a feed medium to the initial medium during culturing, wherein at least one of the initial medium or the feed medium contains an enzymatic degradation product of fish meat or a fish meat extract added thereto. A method of producing a protein of interest using the above culture method.
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公开(公告)号：US20080124761A1
公开(公告)日：2008-05-29
申请号：US11793784
申请日：2005-12-26
Applicant: Susumu Goto , Shohei Kishishita , Shinya Takuma , Chikashi Hirashima
Inventor： Susumu Goto , Shohei Kishishita , Shinya Takuma , Chikashi Hirashima
CPC classification number: C12N5/0037 , C12N2500/80 , C12N2510/02
Abstract: It is an object of the present invention to allow a cell to produce a protein at a high level using a medium containing an enzymatic degradation product of fish meat or a fish meat extract. A method of culturing a cell comprising starting culturing in an initial medium and feeding at least once a feed medium to the initial medium during culturing, wherein at least one of the initial medium or the feed medium contains an enzymatic degradation product of fish meat or a fish meat extract added thereto. A method of producing a protein of interest using the above culture method.
Abstract translation: 本发明的目的是允许细胞使用含有鱼肉或鱼肉提取物的酶降解产物的培养基来产生高水平的蛋白质。 一种培养细胞的方法，包括在初始培养基中开始培养，并且在培养期间至少将进料培养基喂入初始培养基，其中初始培养基或饲料培养基中的至少一种含有鱼肉的酶降解产物或 加入鱼肉提取物。 使用上述培养方法制备感兴趣的蛋白质的方法。
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公开(公告)号：US20110117087A1
公开(公告)日：2011-05-19
申请号：US12911300
申请日：2010-10-25
Applicant: REINHARD FRANZE , Thomas Link , Shinya Takuma , Yoshinori Takagi , Chikashi Hirashima , Yuriko Tsuda
Inventor： REINHARD FRANZE , Thomas Link , Shinya Takuma , Yoshinori Takagi , Chikashi Hirashima , Yuriko Tsuda
IPC: A61K39/395 , C12P21/02 , C07K16/00 , C07K16/28
CPC classification number: C12P21/005 , C07K16/2866 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C07K2317/76
Abstract: Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80% of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.
Abstract translation: 本文报道了一种生产免疫球蛋白的方法，包括以下步骤：a）提供包含编码免疫球蛋白的核酸的真核细胞，b）在培养基中培养真核细胞，其中培养基中可用的葡萄糖量 每个时间单位保持恒定并且限制在每时间单位的细胞在培养基中可最大限度地利用的量的小于80％，和c）从培养物中回收免疫球蛋白。
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公开(公告)号：US20130295613A1
公开(公告)日：2013-11-07
申请号：US13976196
申请日：2011-12-28
Applicant: Shohei Kishishita , Tomoko Okui , Yasuharu Shinoda , Shinya Takuma
Inventor： Shohei Kishishita , Tomoko Okui , Yasuharu Shinoda , Shinya Takuma
IPC: C07K16/28
CPC classification number: C07K16/28 , C07K16/244 , C07K16/2866 , C07K16/303 , C07K2317/14 , C12N5/0602 , C12N2511/00 , C12N2523/00 , C12P21/02
Abstract: While a desired protein is prepared by culturing an animal cell that produces the protein to cause the protein to be produced, level of heterogeneity components of the protein is modulated by performing the culture at a normal culture temperature for a certain period and then continuing the culture at a culture temperature lowered to 25-35° C.
Abstract translation: 虽然通过培养产生蛋白质以产生蛋白质的动物细胞来制备所需的蛋白质，但通过在正常培养温度下进行一定时间的培养，然后继续培养来调节蛋白质的异质性成分的水平 在培养温度降至25-35℃
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