公开(公告)号：US20110046054A1

公开(公告)日：2011-02-24

申请号：US12594007

申请日：2008-03-28

申请人： Aparna Dixit ,  Aruna Vashishta ,  Tejram Sahu ,  Shailesh Kumar Choudhary ,  Alli Murugesan

发明人： Aparna Dixit ,  Aruna Vashishta ,  Tejram Sahu ,  Shailesh Kumar Choudhary ,  Alli Murugesan

IPC分类号： A61K38/16 ,  C07K14/415 ,  C07H21/04 ,  C07K1/14 ,  C07K1/16 ,  C12P21/02 ,  A61P3/10

CPC分类号： C07K14/415 ,  A61K38/00

摘要： The present invention relates to a novel hypoglycemic/anti-hyperglycemic protein named ADMc1 purified from the seeds of Momordica charantia for control of hyperglycemia. The process for the purification of novel hypoglycemic/anti-hyperglycemic protein named ADMc1 is also disclosed. The invention also relates to process for preparation and purification of the recombinant novel hypoglycemic/anti-hyperglycemic protein of Momordica charantia, named rADMc1. Both ADMc1 and rADMc1 are highly effective and need to be administered only once a day to maintain normal blood glucose levels. The procedure involves purification of a novel hypoglycemic/anti-hyperglycemic protein of M. charantia, construction of cDNA library from M. charantia seeds, screening of cDNA library using oligonucleotide probe designed on the basis of amino acid sequence of the tryptic fragment of the protein, cloning of the cDNA in a eukaryotic expression system, expression and purification of the recombinant protein.

摘要翻译： 本发明涉及从苦瓜属的种子中纯化的用于控制高血糖的名为ADMc1的新型降血糖/抗高血糖蛋白。 还公开了名为ADMc1的新型降血糖/抗高血糖蛋白的纯化方法。 本发明还涉及名为rADMc1的苦瓜属重组新型降血糖/抗高血糖蛋白的制备和纯化方法。 ADMc1和rADMc1都是非常有效的，每天只需服用一次，以维持正常的血糖水平。 该方法涉及纯化马氏杆菌的新型低血糖/抗高血糖蛋白，构建来自马鞭草种子的cDNA文库，使用基于蛋白质的胰蛋白酶片段的氨基酸序列设计的寡核苷酸探针筛选cDNA文库 ，在真核表达系统中克隆cDNA，重组蛋白的表达和纯化。

公开(公告)号：US08669348B2

公开(公告)日：2014-03-11

申请号：US12594007

申请日：2008-03-28

申请人： Aparna Dixit ,  Aruna Vashishta ,  Tejram Sahu ,  Shailesh Kumar Choudhary ,  Alli Murugesan

发明人： Aparna Dixit ,  Aruna Vashishta ,  Tejram Sahu ,  Shailesh Kumar Choudhary ,  Alli Murugesan

IPC分类号： A61K38/00 ,  A61K38/10 ,  A61K38/16 ,  A61K38/04 ,  A61P3/10 ,  C07K4/10 ,  C07K1/14 ,  C07K7/08 ,  C07K14/415

CPC分类号： C07K14/415 ,  A61K38/00

摘要： The present invention relates to a novel hypoglycemic/anti-hyperglycemic protein named ADMc1 purified from the seeds of Momordica charantia for control of hyperglycemia. The process for the purification of novel hypoglycemic/anti-hyperglycemic protein named ADMc1 is also disclosed. The invention also relates to process for preparation and purification of the recombinant novel hypoglycemic/anti-hyperglycemic protein of Momordica charantia, named rADMc1. Both ADMc1 and rADMc1 are highly effective and need to be administered only once a day to maintain normal blood glucose levels. The procedure involves purification of a novel hypoglycemic/anti-hyperglycemic protein of M. charantia, construction of cDNA library from M. charantia seeds, screening of cDNA library using oligonucleotide probe designed on the basis of amino acid sequence of the tryptic fragment of the protein, cloning of the cDNA in a eukaryotic expression system, expression and purification of the recombinant protein.

摘要翻译： 本发明涉及从苦瓜属的种子中纯化的用于控制高血糖的名为ADMc1的新型降血糖/抗高血糖蛋白。 还公开了名为ADMc1的新型降血糖/抗高血糖蛋白的纯化方法。 本发明还涉及名为rADMc1的苦瓜属重组新型降血糖/抗高血糖蛋白的制备和纯化方法。 ADMc1和rADMc1都是非常有效的，每天只需服用一次，以维持正常的血糖水平。 该方法涉及纯化马氏杆菌的新型低血糖/抗高血糖蛋白，构建来自马鞭草种子的cDNA文库，使用基于蛋白质的胰蛋白酶片段的氨基酸序列设计的寡核苷酸探针筛选cDNA文库 ，在真核表达系统中克隆cDNA，重组蛋白的表达和纯化。