公开(公告)号：US20080187241A1

公开(公告)日：2008-08-07

申请号：US11769036

申请日：2007-06-27

Applicant: Ronak TALATI ,  Margarida BARROSO

Inventor： Ronak TALATI ,  Margarida BARROSO

IPC: G06K9/20

CPC classification number: G06K9/3233 ,  G06K9/00134

Abstract: Computer-implemented image processing methods and apparatuses are presented for automatically selecting regions of interest within an image represented by pixel intensity values. A first pixel box is employed in progressively scanning and evaluating the image. If pixels within the first pixel box have pixel-intensity-related characteristics exceeding respective defined thresholds, then those pixels are identified as an area of interest and a second pixel box is employed in progressively scanning and evaluating the selected area of interest to identify regions of interest. Each area of interest is larger than a region of interest, and the second pixel box is smaller than the first. Regions of interest within the image are identified if one or more pixel-intensity-related characteristics of pixels within the second pixel box exceeds a second defined threshold, wherein the second threshold is greater than the first. Once selected, identifying information for the regions of interest is stored or output.

Abstract translation: 呈现计算机实现的图像处理方法和装置，用于自动选择由像素强度值表示的图像内的感兴趣区域。 采用第一像素盒逐渐扫描和评估图像。 如果第一像素盒内的像素具有超过相应定义阈值的像素强度相关特征，那么这些像素被识别为感兴趣区域，并且第二像素盒被用于逐渐扫描和评估所选择的感兴趣区域以识别 利益。 感兴趣区域大于感兴趣区域，第二像素盒小于第一像素区域。 如果第二像素框内的像素的一个或多个像素强度相关特征超过第二定义阈值，则识别图像内的感兴趣区域，其中第二阈值大于第一阈值。 一旦被选择，存储或输出关注感兴趣区域的识别信息。

公开(公告)号：US08126267B2

公开(公告)日：2012-02-28

申请号：US11769036

申请日：2007-06-27

Applicant: Ronak Talati ,  Margarida Barroso

Inventor： Ronak Talati ,  Margarida Barroso

IPC: G06K9/38

CPC classification number: G06K9/3233 ,  G06K9/00134

Abstract: Computer-implemented image processing methods and apparatuses are presented for automatically selecting regions of interest within an image represented by pixel intensity values. A first pixel box is employed in progressively scanning and evaluating the image. If pixels within the first pixel box have pixel-intensity-related characteristics exceeding respective defined thresholds, then those pixels are identified as an area of interest and a second pixel box is employed in progressively scanning and evaluating the selected area of interest to identify regions of interest. Each area of interest is larger than a region of interest, and the second pixel box is smaller than the first. Regions of interest within the image are identified if one or more pixel-intensity-related characteristics of pixels within the second pixel box exceeds a second defined threshold, wherein the second threshold is greater than the first. Once selected, identifying information for the regions of interest is stored or output.

Abstract translation: 呈现计算机实现的图像处理方法和装置，用于自动选择由像素强度值表示的图像内的感兴趣区域。 采用第一像素盒逐渐扫描和评估图像。 如果第一像素盒内的像素具有超过相应定义阈值的像素强度相关特征，那么这些像素被识别为感兴趣区域，并且第二像素盒被用于逐渐扫描和评估所选择的感兴趣区域以识别 利益。 感兴趣区域大于感兴趣区域，第二像素盒小于第一像素区域。 如果第二像素框内的像素的一个或多个像素强度相关特征超过第二定义阈值，则识别图像内的感兴趣区域，其中第二阈值大于第一阈值。 一旦被选择，存储或输出关注感兴趣区域的识别信息。

公开(公告)号：US20080227120A1

公开(公告)日：2008-09-18

申请号：US12026413

申请日：2008-02-05

Applicant: Margarida BARROSO ,  Ronak TALATI

Inventor： Margarida BARROSO ,  Ronak TALATI

IPC: G01N33/574

CPC classification number: G06K9/3233 ,  G06K9/00134

Abstract: Automated FRET imaging of membrane-bound receptor/ligand complexes can discriminate between a clustered organization of ligand/receptor complexes that occurs during the early endocytic stages following internalization and a random distribution characteristic of late stage disassociation of ligand from the receptor. In the case of the low density lipoprotein receptor (LDL-R) and its ligand, LDL, this feature of FRET imaging forms the basis of an assay to monitor the endosomal release of cholesterol into the cell and identify compounds which alter pH in the endosome thereby inhibiting the disassociation of ligand and cholesterol from the receptor, a mechanism that is involved in regulation of plasma/serum cholesterol.

Abstract translation: 膜结合受体/配体复合物的自动FRET成像可以区分在内化之后的早期内吞阶段期间发生的配体/受体复合物的聚集组织以及配体与受体的晚期解离的随机分布特征。 在低密度脂蛋白受体（LDL-R）及其配体LDL的情况下，FRET成像的这一特征构成了监测胆固醇进入细胞的内体体外释放的测定的基础，并鉴定了改变内在体内pH值的化合物 从而抑制配体和胆固醇与受体的分离，这是参与血浆/血清胆固醇调节的机制。

公开(公告)号：US08609329B2

公开(公告)日：2013-12-17

申请号：US12026413

申请日：2008-02-05

Applicant: Margarida Barroso ,  Ronak Talati

Inventor： Margarida Barroso ,  Ronak Talati

IPC: C12Q1/00

CPC classification number: G06K9/3233 ,  G06K9/00134

Abstract: Automated FRET imaging of membrane-bound receptor/ligand complexes can discriminate between a clustered organization of ligand/receptor complexes that occurs during the early endocytic stages following internalization and a random distribution characteristic of late stage disassociation of ligand from the receptor. In the case of the low density lipoprotein receptor (LDL-R) and its ligand, LDL, this feature of FRET imaging forms the basis of an assay to monitor the endosomal release of cholesterol into the cell and identify compounds which alter pH in the endosome thereby inhibiting the disassociation of ligand and cholesterol from the receptor, a mechanism that is involved in regulation of plasma/serum cholesterol.

Abstract translation: 膜结合受体/配体复合物的自动FRET成像可以区分在内化之后的早期内吞阶段期间发生的配体/受体复合物的聚集组织以及配体与受体的晚期解离的随机分布特征。 在低密度脂蛋白受体（LDL-R）及其配体LDL的情况下，FRET成像的这一特征构成了监测胆固醇进入细胞的内体体外释放的测定的基础，并鉴定了改变内在体内pH值的化合物 从而抑制配体和胆固醇与受体的分离，这是参与血浆/血清胆固醇调节的机制。