公开(公告)号：US20140113373A1

公开(公告)日：2014-04-24

申请号：US14057479

申请日：2013-10-18

申请人： Karen B. Chien ,  Ramille N. Shah

发明人： Karen B. Chien ,  Ramille N. Shah

IPC分类号： C12N5/0775

CPC分类号： A61K47/42 ,  B33Y10/00 ,  C12N5/0068 ,  C12N2521/00 ,  C12N2533/50 ,  C12N2535/00 ,  C12N2537/10

摘要： Porous soy protein-based scaffolds and methods for making the scaffolds using 3D printing techniques are provided. Also provided are tissue growth scaffolds comprising the porous soy protein-based scaffolds and methods for growing tissue on the tissue growth scaffolds. The porous soy protein-containing scaffold comprises a plurality of layers configured in a vertical stack, each layer comprising a plurality of strands comprising denatured soy proteins.

摘要翻译： 提供了多孔大豆蛋白质的支架和使用3D印刷技术制备支架的方法。 还提供了包含多孔大豆蛋白质支架的组织生长支架和用于在组织生长支架上生长组织的方法。 含多孔大豆蛋白的支架包括以垂直堆叠构造的多个层，每个层包含多个包含变性大豆蛋白的链。

公开(公告)号：US08450271B2

公开(公告)日：2013-05-28

申请号：US12759683

申请日：2010-04-13

申请人： Ramille N. Shah ,  Nirav A. Shah ,  Samuel I. Stupp

发明人： Ramille N. Shah ,  Nirav A. Shah ,  Samuel I. Stupp

IPC分类号： A61K38/18 ,  A61K38/00 ,  A61K38/04 ,  C07K14/495 ,  C07K5/00 ,  A61P19/00

CPC分类号： C07K14/00 ,  A61K38/1841 ,  C07K7/06 ,  A61K2300/00

摘要： Disclosed herein are novel peptide amphiphile molecules and compositions composed of a peptide sequence that non-covalently binds the growth factor TGF-β1. Also disclosed are methods of using these peptide amphiphiles to create a gel scaffold in situ that enhances articular cartilage regeneration when used in combination with microfracture. Significant improvement in tissue quality and overall O'Driscoll histological scores were observed in rabbits with full thickness articular cartilage defects treated with the TGF-binding peptide amphiphile. The gel can further serve as a delivery vehicle for recombinant TGF-β1 protein growth factor. Scaffolds that localize and retain chondrogenic growth factors may synergistically enhance cartilage repair when combined with microfracture, by inducing bone marrow mesenchymal stem cells into chondrogenic differentiation. This invention represents a promising new biomimetic approach to enhance current techniques of articular cartilage regeneration in the clinical setting.

摘要翻译： 本文公开了新型肽两亲物分子和由非共价结合生长因子TGF-β1的肽序列组成的组合物。 还公开了使用这些肽两亲物来制备原位凝胶支架的方法，当与微裂缝组合使用时，其可增强关节软骨再生。 在用TGF-结合肽两亲物处理的全层关节软骨缺损的兔子中观察到组织质量和总体O'Driscoll组织学评分的显着改善。 该凝胶可进一步用作重组TGF-β1蛋白生长因子的递送载体。 定位和保留软骨形成生长因子的支架可以通过将骨髓间充质干细胞诱导成软骨形成分化来协同增强软骨修复与微裂缝结合。 本发明代表了在临床环境中增强关节软骨再生的当前技术的有希望的新的仿生方法。

公开(公告)号：US20100266557A1

公开(公告)日：2010-10-21

申请号：US12759683

申请日：2010-04-13

申请人： Ramille N. Shah ,  Nirav A. Shah ,  Samuel I. Stupp

发明人： Ramille N. Shah ,  Nirav A. Shah ,  Samuel I. Stupp

IPC分类号： A61K35/12 ,  A61K38/18 ,  A61P19/00 ,  A61K38/16 ,  A61K38/08 ,  C07K14/00 ,  C07K7/06

CPC分类号： C07K14/00 ,  A61K38/1841 ,  C07K7/06 ,  A61K2300/00

摘要： Disclosed herein are novel peptide amphiphile molecules and compositions composed of a peptide sequence that non-covalently binds the growth factor TGF-β1. Also disclosed are methods of using these peptide amphiphiles to create a gel scaffold in situ that enhances articular cartilage regeneration when used in combination with microfracture. Significant improvement in tissue quality and overall O'Driscoll histological scores were observed in rabbits with full thickness articular cartilage defects treated with the TGF-binding peptide amphiphile. The gel can further serve as a delivery vehicle for recombinant TGF-β1 protein growth factor. Scaffolds that localize and retain chondrogenic growth factors may synergistically enhance cartilage repair when combined with microfracture, by inducing bone marrow mesenchymal stem cells into chondrogenic differentiation. This invention represents a promising new biomimetic approach to enhance current techniques of articular cartilage regeneration in the clinical setting.

摘要翻译： 本文公开了新型肽两亲物分子和由非共价结合生长因子TGF-β1的肽序列组成的组合物。 还公开了使用这些肽两亲物来制备原位凝胶支架的方法，当与微裂缝组合使用时，其可增强关节软骨再生。 在用TGF-结合肽两亲物处理的全层关节软骨缺损的兔子中观察到组织质量和总体O'Driscoll组织学评分的显着改善。 该凝胶可进一步用作重组TGF-β1蛋白生长因子的递送载体。 定位和保留软骨形成生长因子的支架可以通过将骨髓间充质干细胞诱导成软骨形成分化来协同增强软骨修复与微裂缝结合。 本发明代表了在临床环境中增强关节软骨再生的当前技术的有希望的新的仿生方法。