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公开(公告)号：US20080118968A1
公开(公告)日：2008-05-22
申请号：US11312734
申请日：2005-12-21
CPC分类号： C07K14/405
摘要： Expressed Sequence Tags (ESTs) isolated from the unicellular red algae, Cyanidium caldarium, are disclosed. The invention encompasses nucleic acid molecules that encode Cyanidium caldarium protein homologs and fragments therof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.
摘要翻译： 公开了从单细胞红藻（Cyanidium caldarium）分离的表达序列标签（ESTs）。 本发明包括编码Cyanidium caldarium蛋白质同系物并且片段化的核酸分子。 此外，本发明涵盖能够结合蛋白质的抗体。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。
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公开(公告)号：US06476212B1
公开(公告)日：2002-11-05
申请号：US09313294
申请日：1999-05-14
IPC分类号： C07H2100
CPC分类号： C07K14/415
摘要： The present invention provides purified, corn ear-derived polynucleotides (cdps) which encode corn ear-derived polypeptides (CDPs). The invention also provides for the use of cdps or their complements, oligonucleotides, or fragments in methods for determining altered gene expression, to recover regulatory elements, and to follow inheritance of desirable characteristics through hybrid breeding programs. The invention further provides for vectors and host cells containing cdps for the expression of CDPs. The invention additionally provides for (i) use of isolated and purified CDPs to induce antibodies and to screen libraries of compounds and (ii) use of anti-CDP antibodies in diagnostic assays.
摘要翻译： 本发明提供了编码玉米穗衍生多肽（CDP）的纯化的玉米穗衍生多核苷酸（cdps）。 本发明还提供了在用于确定改变的基因表达，恢复调节元件以及通过杂交育种程序追踪所需特征的遗传的方法中使用cdps或其补体，寡核苷酸或片段。 本发明进一步提供含有CDP表达的CDP的载体和宿主细胞。 本发明另外提供（i）使用分离和纯化的CDP诱导抗体和筛选化合物文库和（ii）在诊断测定中使用抗CDP抗体。
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3.发明授权Nucleic acid molecule SEQ ID NO. 68811 and other molecules associated with plants 有权核酸分子SEQ ID NO： 68811和与植物相关的其他分子公开(公告)号：US07560542B2
公开(公告)日：2009-07-14
申请号：US11353150
申请日：2006-02-14
CPC分类号： C07K14/415 , C12N15/8242 , C12N15/8261 , Y02A40/146
摘要： Expressed Sequence Tags (ESTs) isolated from maize are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.
摘要翻译： 公开了从玉米分离的表达序列标签（ESTs）。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 所公开的EST可用于开发用于了解关键植物发育和代谢途径的新策略。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 使用本发明提供的EST的序列同源性分析将导致对所需农学性状的更有效的基因筛选。 这些植物基因组学难题的这些精选片段的不断扩展的数据库将快速扩展后续功能验证所必需的知识，这是当前植物生物技术努力的关键限制。
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公开(公告)号：US20080206841A1
公开(公告)日：2008-08-28
申请号：US11239592
申请日：2005-09-30
CPC分类号： C07K14/405
摘要： Expressed Sequence Tags (ESTs) isolated from the unicellular green algae, Chlorella vulgaris, are disclosed. The invention encompasses nucleic acid molecules that encode Chlorella protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.
摘要翻译： 公开了从单细胞绿藻，寻常型小球藻分离的表达序列标签（ESTs）。 本发明包括编码小球藻蛋白同源物及其片段的核酸分子。 此外，本发明涵盖能够结合蛋白质的抗体。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。
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公开(公告)号：US08124844B2
公开(公告)日：2012-02-28
申请号：US11980366
申请日：2007-10-31
申请人： Thomas J. La Rosa , David K. Kovalic , Yihua Zhou , Yongwei Cao , Mark S. Abad , Scott E. Andersen , Robert E. Buehler , Joseph R. Byrum , Nordine Cheikh , Timothy W. Conner , Brian E. Coombs , Stanton B. Dotson , Patrice Dubois , Michael D. Edgerton , Dane K. Fisher , Kristine J. Hardeman , Gregory J. Heck , Gregory J. Hinkle , Raghunath V. Lalgudi , Jingdong Liu , Linda L. Lutfiyya , Debbie A. Mahadeo , James D. Masucci , James D. McIninch , Philip W. Miller , Donald E. Nelson , Ming Peng , Thomas G. Ruff , Hridayabhiranjan Shukla , Joshua Stein , Michael D. Thompson , Kunsheng Wu
发明人： Thomas J. La Rosa , David K. Kovalic , Yihua Zhou , Yongwei Cao , Mark S. Abad , Scott E. Andersen , Robert E. Buehler , Joseph R. Byrum , Nordine Cheikh , Timothy W. Conner , Brian E. Coombs , Stanton B. Dotson , Patrice Dubois , Michael D. Edgerton , Dane K. Fisher , Kristine J. Hardeman , Gregory J. Heck , Gregory J. Hinkle , Raghunath V. Lalgudi , Jingdong Liu , Linda L. Lutfiyya , Debbie A. Mahadeo , James D. Masucci , James D. McIninch , Philip W. Miller , Donald E. Nelson , Ming Peng , Thomas G. Ruff , Hridayabhiranjan Shukla , Joshua Stein , Michael D. Thompson , Kunsheng Wu
IPC分类号： C12N15/00 , A01H5/00 , A01H1/00 , C07H21/04 , C07K14/415
摘要： Polynucleotides useful for improvement of plants are provided. In particular, polynucleotide sequences are provided from plant sources. Polypeptides encoded by the polynucleotide sequences are also provided. The disclosed polynucleotides and polypeptides find use in production of transgenic plants to produce plants having improved properties.
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公开(公告)号：US20100050302A1
公开(公告)日：2010-02-25
申请号：US12611005
申请日：2009-11-02
CPC分类号： C07K14/405
摘要： Expressed Sequence Tags (ESTs) isolated from the unicellular red algae, Cyanidium caldarium, are disclosed. The invention encompasses nucleic acid molecules that encode Cyanidium caldarium protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.
摘要翻译： 公开了从单细胞红藻（Cyanidium caldarium）分离的表达序列标签（ESTs）。 本发明包括编码Cyanidium caldarium蛋白质同系物及其片段的核酸分子。 此外，本发明涵盖能够结合蛋白质的抗体。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。
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公开(公告)号：US20080263730A1
公开(公告)日：2008-10-23
申请号：US11353150
申请日：2006-02-14
IPC分类号： A01H5/00 , C07K14/415 , C12N5/04 , C07H21/04
CPC分类号： C07K14/415 , C12N15/8242 , C12N15/8261 , Y02A40/146
摘要： Expressed Sequence Tags (ESTs) isolated from maize are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.
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公开(公告)号：US20080134369A1
公开(公告)日：2008-06-05
申请号：US11980757
申请日：2007-10-31
CPC分类号： C07K14/415
摘要： The present invention is in the field of plant biochemistry. More specifically the invention relates to nucleic acid molecules that encode proteins and fragments of proteins produced in plant cells, in particular, maize plants. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins.
摘要翻译： 本发明在植物生物化学领域。 更具体地，本发明涉及编码在植物细胞，特别是玉米植物中产生的蛋白质和蛋白质片段的核酸分子。 本发明还涉及如此编码的蛋白质和蛋白质的片段以及能够结合蛋白质的抗体。 本发明还涉及使用核酸分子，蛋白质和蛋白质片段的方法。
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公开(公告)号：US20070277258A1
公开(公告)日：2007-11-29
申请号：US11227183
申请日：2005-09-16
IPC分类号： A01H5/00 , C07H21/04 , C07K14/415
CPC分类号： C07K14/415
摘要： The present invention is in the field of plant biochemistry. More specifically the invention relates to nucleic acid molecules that encode proteins and fragments of proteins produced in plant cells, in particular, maize plants. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins.
摘要翻译： 本发明在植物生物化学领域。 更具体地，本发明涉及编码在植物细胞，特别是玉米植物中产生的蛋白质和蛋白质片段的核酸分子。 本发明还涉及如此编码的蛋白质和蛋白质的片段以及能够结合蛋白质的抗体。 本发明还涉及使用核酸分子，蛋白质和蛋白质片段的方法。
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公开(公告)号：US20090288223A1
公开(公告)日：2009-11-19
申请号：US12477044
申请日：2009-06-02
CPC分类号： C07K14/405
摘要： Expressed Sequence Tags (ESTs) isolated from the unicellular green algae, Chlorella vulgaris, are disclosed. The invention encompasses nucleic acid molecules that encode Chlorella protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.
摘要翻译： 公开了从单细胞绿藻，寻常型小球藻分离的表达序列标签（ESTs）。 本发明包括编码小球藻蛋白同源物及其片段的核酸分子。 此外，本发明涵盖能够结合蛋白质的抗体。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。
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