METHOD FOR MANUFACTURING COFFEE BY SOLID STATE FERMENTATION
    3.
    发明申请
    METHOD FOR MANUFACTURING COFFEE BY SOLID STATE FERMENTATION 审中-公开
    通过固体发酵制造咖啡的方法

    公开(公告)号:US20100239711A1

    公开(公告)日:2010-09-23

    申请号:US12408737

    申请日:2009-03-23

    CPC classification number: A23F5/02

    Abstract: A method is provided for manufacturing coffee by solid state fermentation, including the following steps: depositing coffee beans in a dust-free clean container; propagating a fungus with solid state fermentation; carrying out implantation process, wherein the fungus is implanted to the coffee beans contained in the dust-free clean container with a sterile operation, the fungus being one belonging to Eumycota, including at least one selected from Basidiomycotina and Ascomycotina; and performing a fermentation process. Also provided is a formula of the solid cultivation medium used in the method, which is suitable for both large-scale and small-scale production of fungi and is applicable to most fungi to not only increase the throughput as desired, but also provide a metabolic product containing pharmacologically active ingredients.

    Abstract translation: 提供了一种通过固态发酵制造咖啡的方法,包括以下步骤:将咖啡豆放在无尘的清洁容器中; 用固态发酵繁殖真菌; 进行植入过程,其中将真菌植入无菌操作的无尘清洁容器中所含的咖啡豆中,所述真菌属于真菌属(Eumycota),其包括选自担子菌纲和子囊菌纲中的至少一种; 并进行发酵过程。 还提供了该方法中使用的固体培养基的配方,其适用于大规模生产和小规模生产真菌,并且适用于大多数真菌,不仅根据需要增加产量,而且还提供代谢 含有药理活性成分的产品。

    Method for propagating fungi using solid state fermentation
    4.
    发明授权
    Method for propagating fungi using solid state fermentation 失效
    使用固态发酵繁殖真菌的方法

    公开(公告)号:US06558943B1

    公开(公告)日:2003-05-06

    申请号:US09655435

    申请日:2000-09-05

    CPC classification number: C12P33/00 C12N1/14 C12P19/38

    Abstract: A solid state fermentation (SSF) method is provided which is effective for both small- and large-scale fungal cultivation. Also provided is SSF media for fungal cultivation. The media preferably contains a carbon source and nitrogen source to provide a carbon to nitrogen ratio of about 5:1 to about 25:1 by weight. The media may also contain a vitamin and an inorganic substance. A preferred SSF medium contains malt extract, yeast extract, peptone, glucose, water, solid base, and calcium carbonate or gypsum. Before propagating fungal mycelia in the SSF medium, the mycelia may be pre-cultivated in a solid culture medium and then in a liquid medium. Although the SSF method can be used in growing most fungi, preferred fungi include Cordyceps sinensis, Ganoderma lucidum, Antrodia camphorata, Trametes versicolor, and Agaricus blazei. The SSF method not only produces high yield of fungi, but also stimulates the production of fungal metabolites, particularly the kinds with pharmaceutical and medicinal activities. Cordyceps sinensis is preferably grown to produce the active compound H1A which is a derivative of ergosterol.

    Abstract translation: 提供了一种对小型和大型真菌培养有效的固态发酵(SSF)方法。 还提供了SSF培养基用于真菌培养。 介质优选含有碳源和氮源,以提供约5:1至约25:1重量比的碳氮比。 培养基还可以含有维生素和无机物质。 优选的SSF培养基包含麦芽提取物,酵母提取物,蛋白胨,葡萄糖,水,固体碱和碳酸钙或石膏。 在SSF培养基中繁殖真菌菌丝体之前,菌丝体可以在固体培养基中,然后在液体培养基中预培养。 尽管SSF方法可用于种植大多数真菌,但优选的真菌包括冬虫夏草,灵芝,灵芝,梧桐和姬松茸。 SSF方法不仅产生高产量的真菌,而且刺激真菌代谢物的生产,特别是药物和药物活性的种类。 冬虫夏草优选生长以产生作为麦角固醇衍生物的活性化合物H1A。

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