公开(公告)号：US20110306751A1

公开(公告)日：2011-12-15

申请号：US13122547

申请日：2009-10-05

申请人： Monica J. Roth ,  William M. Schneider ,  Gaetano T. Montelione ,  Masayori Inouye ,  Yuefeng Tang

发明人： Monica J. Roth ,  William M. Schneider ,  Gaetano T. Montelione ,  Masayori Inouye ,  Yuefeng Tang

IPC分类号： C07K2/00 ,  C12N1/21 ,  C12P21/00

CPC分类号： C12N15/72 ,  C07K14/31 ,  C07K2319/02 ,  C12N1/20 ,  C12N9/22 ,  C12N15/635 ,  C12N15/67 ,  C12Y301/00 ,  H05K999/99

摘要： The present invention is directed to the improved methods for the temporal induction of proteins using the condensed single protein production (cSPP) system.

摘要翻译： 本发明涉及使用缩合单蛋白质生产（cSPP）系统来时间诱导蛋白质的改进方法。

公开(公告)号：US4943531A

公开(公告)日：1990-07-24

申请号：US731128

申请日：1985-05-06

申请人： Stephen P. Goff ,  Naoko Tanese ,  Monica J. Roth

发明人： Stephen P. Goff ,  Naoko Tanese ,  Monica J. Roth

IPC分类号： C07K14/15 ,  C12N9/12 ,  C12N15/54

CPC分类号： C07K14/005 ,  C12N9/1276 ,  C07K2319/00 ,  C12N2740/13022

摘要： This invention provides a plasmic which, when introduced into a suitable host cell and grown under appropriate conditions, effects expression of a gene on the plasmid and production of a polypeptide having reverse transcriptase activity. The plasmid is a double-stranded DNA molecule which includes in a 5' to 3' order the following: a DNA sequence which includes an inducible promoter; a DNA sequence which includes an ATG initiation condon; the central portion of the Moloney murine leukemia virus (MuLV) pol gene, said central portion including a DNA sequence which encodes the polypeptide having reverse transcriptase activity; a DNA sequence which contains a gene associated with a selectable or identifiable phenotypic trait which is manifested when the vector is present in the host cell; and a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell.The invention also concerns a method for recovering purified enzymatically-active polypeptide having reverse transcriptase activity, the polypeptide being encoded by the plasmid pB6 B15.23, from a suitable host cell e.g., E. coli HB101 producing the polypeptide. Finally, the invention concerns use of the polypeptide to prepare complementary DNA (cDNA).

摘要翻译： 本发明提供了一种当合适的宿主细胞被引入并在合适的条件下生长时，影响质粒表达并产生具有逆转录酶活性的多肽的质粒。 质粒是双链DNA分子，其以5'至3'的顺序包括以下：包含诱导型启动子的DNA序列; 包括ATG起始细菌的DNA序列; 所述莫洛尼鼠白血病病毒（MuLV）pol基因的中心部分，所述中心部分包​​括编码具有逆转录酶活性的多肽的DNA序列; 包含与可选择或可鉴定的表型性状相关的基因的DNA序列，其在载体存在于宿主细胞中时表现出来; 以及含有能够在宿主细胞中自主复制的细菌质粒的复制起点的DNA序列。 本发明还涉及从合适的宿主细胞例如产生多肽的大肠杆菌HB101回收具有逆转录酶活性的纯化的酶促活性多肽的方法，该多肽由质粒pB6 B15.23编码。 最后，本发明涉及多肽制备互补DNA（cDNA）的应用。