公开(公告)号：US07335492B2

公开(公告)日：2008-02-26

申请号：US11252753

申请日：2005-10-19

申请人： Merja Penttilä ,  Tiina Nakari-Setälä ,  Richard Fagerström ,  Klaus Selber ,  Maria-Regina Kula ,  Markus Linder ,  Folke Tjerneld

发明人： Merja Penttilä ,  Tiina Nakari-Setälä ,  Richard Fagerström ,  Klaus Selber ,  Maria-Regina Kula ,  Markus Linder ,  Folke Tjerneld

IPC分类号： C12N1/00 ,  A61K38/00

CPC分类号： C07K7/06 ,  C07K1/14 ,  C07K7/08 ,  C07K14/37 ,  C07K2319/00 ,  C07K2319/02

摘要： The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.

摘要翻译： 本发明涉及水相两相体系（ATPS）中蛋白质的分离和纯化。 具体地，本发明提供了通过将所述蛋白质融合到具有携带所述蛋白质到其中一个相的能力的靶向蛋白质来分配ATPS中感兴趣的蛋白质的方法。

公开(公告)号：US09719064B2

公开(公告)日：2017-08-01

申请号：US13814235

申请日：2011-08-01

申请人： Klaus Selber ,  Bernhard Weingaertner ,  Hermann Wehlmann ,  Winfried Rosen ,  Alfred Pühler ,  Patrick Schwientek ,  Jörn Kalinowski ,  Udo Wehmeier

发明人： Klaus Selber ,  Bernhard Weingaertner ,  Hermann Wehlmann ,  Winfried Rosen ,  Alfred Pühler ,  Patrick Schwientek ,  Jörn Kalinowski ,  Udo Wehmeier

IPC分类号： C12N1/20 ,  C07K14/365 ,  C12P19/26

CPC分类号： C12N1/20 ,  C07K14/365 ,  C12P19/26

摘要： The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type—and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene- and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield.

公开(公告)号：US07060669B1

公开(公告)日：2006-06-13

申请号：US09936823

申请日：2000-03-24

申请人： Merja Penttilä ,  Tiina Nakari-Setälä ,  Richard Fagerström ,  Klaus Selber ,  Maria-Regina Kula ,  Markus Linder ,  Folke Tjerneld

发明人： Merja Penttilä ,  Tiina Nakari-Setälä ,  Richard Fagerström ,  Klaus Selber ,  Maria-Regina Kula ,  Markus Linder ,  Folke Tjerneld

IPC分类号： A61K38/00

CPC分类号： C07K7/06 ,  C07K1/14 ,  C07K7/08 ,  C07K14/37 ,  C07K2319/00 ,  C07K2319/02

摘要： The present invention relates to isolation and purification of proteins in aqueous two-phase systems (ATPS). Specifically the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.

摘要翻译： 本发明涉及水相两相体系（ATPS）中蛋白质的分离和纯化。 具体地说，本发明提供了通过将所述蛋白质融合到具有携带所述蛋白质到其中一个相的能力的靶向蛋白质来分配ATPS中感兴趣的蛋白质的方法。

公开(公告)号：US20060084164A1

公开(公告)日：2006-04-20

申请号：US11252753

申请日：2005-10-19

申请人： Merja Penttila ,  Tiina Nakari-Setala ,  Richard Fagerstrom ,  Klaus Selber ,  Maria-Regina Kula ,  Markus Linder ,  Folke Tjerneld

发明人： Merja Penttila ,  Tiina Nakari-Setala ,  Richard Fagerstrom ,  Klaus Selber ,  Maria-Regina Kula ,  Markus Linder ,  Folke Tjerneld

IPC分类号： C12N1/21 ,  C07K16/18 ,  C12N15/74 ,  C12N9/42 ,  C12N9/48

CPC分类号： C07K7/06 ,  C07K1/14 ,  C07K7/08 ,  C07K14/37 ,  C07K2319/00 ,  C07K2319/02

摘要： The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.

摘要翻译： 本发明涉及水相两相体系（ATPS）中蛋白质的分离和纯化。 具体地，本发明提供了通过将所述蛋白质融合到具有携带所述蛋白质到其中一个相的能力的靶向蛋白质来分配ATPS中感兴趣的蛋白质的方法。

公开(公告)号：US20130302855A1

公开(公告)日：2013-11-14

申请号：US13814235

申请日：2011-08-01

申请人： Klaus Selber ,  Bernhard Weingaertner ,  Hermann Wehlmann ,  Winfried Rosen ,  Alfred Puhler ,  Patrick Schwientek ,  Jörn Kalinowski ,  Udo Wehmeier

发明人： Klaus Selber ,  Bernhard Weingaertner ,  Hermann Wehlmann ,  Winfried Rosen ,  Alfred Puhler ,  Patrick Schwientek ,  Jörn Kalinowski ,  Udo Wehmeier

IPC分类号： C12N1/20 ,  C12P19/26

CPC分类号： C12N1/20 ,  C07K14/365 ,  C12P19/26

摘要： The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type-and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene-and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield.

摘要翻译： 本发明描述了野生型基因组的DNA序列以及引入到野生型基因中的所有遗传修饰以及进一步开发的菌株。 因此，已经完成了开发菌株的第一个基因型表征，包括最新的生产菌株，占本发明的主要部分。 此外，在确定的DNA序列的基础上，鉴定潜在的基因并结合其功能注释，与本发明的另一部分相结合。 特别地，基因和DNA序列以及从那里导出的蛋白质序列有助于在整个应变发展过程中受诱变修饰影响的本发明，潜在地有助于提高产量。