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    Process and device for the screening of molecules with regard to their individual binding behaviour towards at least one given ligand
    1.
    发明授权
    Process and device for the screening of molecules with regard to their individual binding behaviour towards at least one given ligand 失效
    关于它们对至少一个给定配体的单独结合行为的分子筛选的方法和装置

    公开(公告)号：US06713264B2

    公开(公告)日：2004-03-30

    申请号：US08891778

    申请日：1997-07-14

    Applicant: Klaus Luttermann ,  Edgar Diessel ,  Winfried Kosch ,  Walter Weichel

    Inventor： Klaus Luttermann ,  Edgar Diessel ,  Winfried Kosch ,  Walter Weichel

    IPC: G01N3353

    CPC classification number: C12M47/04 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/0072 ,  G01N15/1468 ,  Y10S435/808

    Abstract: The process is used for the screening of molecules from molecule libraries with regard to their individual binding behavior towards at least one given ligand. For this purpose the ligands labelled with a fluorescent dye are mixed with the molecule library which is in the form of a suspension. The mixture is plated out on a two-dimensional substrate (2) after the excess, unbound ligands have been washed out. Then the local fluorescence intensities on the substrate are electro-optically identified in a fluorescence microscope (5) and electronically discriminated in accordance with given selection criteria. The objects selected and localised in this way are then sequentially positioned exactly by a displacement, the coordinates of which are controlled by the image calculator, between the substrate (2) and a separation actuator (20, 21) and are spatially separated from the substrate (2) by the separation actuator (20, 21).

    Abstract translation: 该方法用于从分子文库筛选针对至少一种给定配体的单个结合行为的分子。 为此，用荧光染料标记的配体与悬浮液形式的分子文库混合。 在过量的未结合配体被洗掉之后，将混合物镀在二维底物（2）上。 然后在荧光显微镜（5）中对基底上的局部荧光强度进行电光学鉴定，并根据给定的选择标准进行电子鉴别。 以这种方式选择和定位的物体然后通过基板（2）和分离致动器（​​20,21）之间的位移（其坐标由图像计算器控制）精确地顺序地定位并且在空间上与基板分离 （2）通过分离致动器（​​20,21）。

    Device for separating micro objects
    2.
    发明授权
    Device for separating micro objects 失效
    用于分离微物体的装置

    公开(公告)号：US06517779B1

    公开(公告)日：2003-02-11

    申请号：US09214839

    申请日：1999-01-13

    Applicant: Klaus Luttermann ,  Edgar Diessel ,  Markus Weidauer

    Inventor： Klaus Luttermann ,  Edgar Diessel ,  Markus Weidauer

    IPC: B01L302

    CPC classification number: C12M47/04 ,  B01L3/021 ,  C12M35/00

    Abstract: The device serves for the transfer of microobjects, in particular of biological objects, from a current substrate (9) to a target substrate. In this case, use is made of a displaceable pipette system which is connected to a pressure generating device for aspirating in and flushing out the biological objects. The pipette system is disposed in a micromanipulator (3) and consists mainly of a substantially vertically disposed microcapillary (1) having a clear width of 1 &mgr;m to 50 &mgr;m.

    Abstract translation: 该装置用于将微物体，特别是生物物体从当前基底（9）转移到目标基底。 在这种情况下，使用可移位移液管系统，其连接到用于吸入和冲洗生物体的压力产生装置。 移液管系统设置在显微操纵器（3）中，主要由基本上垂直设置的微毛细管（1）组成，微毛细管（1）的清晰宽度为1〜50μm。

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