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    Rice regulatory sequences for gene expression in defined wheat tissue
    1.
    发明授权
    Rice regulatory sequences for gene expression in defined wheat tissue 有权
    用于定义小麦组织中基因表达的水稻调节序列

    公开(公告)号：US07541452B2

    公开(公告)日：2009-06-02

    申请号：US12080205

    申请日：2008-04-01

    申请人： Martin Urban ,  Rebecca Stratford ,  Kim Hammond-Kosack ,  Pierre Lecocq ,  Richard Kemp

    发明人： Martin Urban ,  Rebecca Stratford ,  Kim Hammond-Kosack ,  Pierre Lecocq ,  Richard Kemp

    IPC分类号： C07H21/04 ,  C12N15/82 ,  A01H5/00

    CPC分类号： A01H5/00 ,  C07K14/415 ,  C12N15/8222 ,  C12N15/8225 ,  C12N15/8234

    摘要： The abundance of the 96 most abundant EST cluster sequences in a wheat lemma/palea cDNA library was investigated in a range of cDNA libraries made from various wheat tissues. 30 cDNA sequences showing highly enhanced abundance in lemma, palea and glume tissues over leaf, stem, embryo, endosperm and root tissue were selected for further analysis. These wheat EST cluster sequences were used to identify rice cDNA homologs. The abundance of the rice cDNA homologs was compared in rice leaf and panicle (includes lemma and palea) cDNA libraries. Rice cDNAs showing preferential expression in the panicle were then used to identify homologous rice genomic DNA clones, the putative promoter sequences have been identified and cloned.

    摘要翻译： 在一系列由各种小麦组织制成的cDNA文库中，研究了小麦引物/外来cDNA文库中96个最丰富的EST簇序列的丰度。 选择30个cDNA序列，显示叶，茎，胚，胚乳和根组织中引物，内脏和叶片组织的丰度丰度，进一步分析。 这些小麦EST簇序列用于鉴定水稻cDNA同源物。 在水稻和穗（包括外来和外来）cDNA文库中比较了水稻cDNA同源物的丰度。 然后使用在穗中显示优先表达的水稻cDNA来鉴定同源的水稻基因组DNA克隆，推定的启动子序列已被鉴定和克隆。

    Rice regulatory sequences for gene expression in defined wheat tissue
    4.
    发明申请
    Rice regulatory sequences for gene expression in defined wheat tissue 有权
    用于定义小麦组织中基因表达的水稻调节序列

    公开(公告)号：US20070130633A1

    公开(公告)日：2007-06-07

    申请号：US10488149

    申请日：2002-08-23

    申请人： Martin Urban ,  Rebecca Stratford ,  Kim Hammond-Kosack ,  Richard Kemp ,  Pierre Lecocq

    发明人： Martin Urban ,  Rebecca Stratford ,  Kim Hammond-Kosack ,  Richard Kemp ,  Pierre Lecocq

    IPC分类号： A01H5/00 ,  C12N5/04 ,  C12N15/82 ,  A01H1/00

    CPC分类号： A01H5/00 ,  C07K14/415 ,  C12N15/8222 ,  C12N15/8225 ,  C12N15/8234

    摘要： The abundance of the 96 most abundant EST cluster sequences in a wheat lemma/palea cDNA library was investigated in a range of cDNA libraries made from various wheat tissues. 30 cDNA sequences showing highly enhanced abundance in lemma, palea and glume tissues over leaf, stem, embryo, endosperm and root tissue were selected for further analysis. These wheat EST cluster sequences were used to identify rice cDNA homologs. The abundance of the rice cDNA homologs was compared in rice leaf and panicle (includes lemma and palea) cDNA libraries. Rice cDNAs showing preferential expression in the panicle were then used to identify homologous rice genomic DNA clones, the putative promoter sequences have been identified and cloned.

    摘要翻译： 在一系列由各种小麦组织制成的cDNA文库中，研究了小麦引物/外来cDNA文库中96个最丰富的EST簇序列的丰度。 选择30个cDNA序列，显示叶，茎，胚，胚乳和根组织中引物，内脏和叶片组织的丰度丰度，进一步分析。 这些小麦EST簇序列用于鉴定水稻cDNA同源物。 在水稻和穗（包括外来和外来）cDNA文库中比较了水稻cDNA同源物的丰度。 然后使用在穗中显示优先表达的水稻cDNA来鉴定同源的水稻基因组DNA克隆，推定的启动子序列已被鉴定和克隆。

    Rice regulatory sequences for gene expression in defined wheat tissue
    5.
    发明申请
    Rice regulatory sequences for gene expression in defined wheat tissue 有权
    用于定义小麦组织中基因表达的水稻调节序列

    公开(公告)号：US20080271206A1

    公开(公告)日：2008-10-30

    申请号：US12080205

    申请日：2008-04-01

    申请人： Martin Urban ,  Rebecca Stratford ,  Kim Hammond-Kosack ,  Pierre Lecocq ,  Richard Kemp

    发明人： Martin Urban ,  Rebecca Stratford ,  Kim Hammond-Kosack ,  Pierre Lecocq ,  Richard Kemp

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  A01H5/00

    CPC分类号： A01H5/00 ,  C07K14/415 ,  C12N15/8222 ,  C12N15/8225 ,  C12N15/8234

    摘要： The abundance of the 96 most abundant EST cluster sequences in a wheat lemma/palea cDNA library was investigated in a range of cDNA libraries made from various wheat tissues. 30 cDNA sequences showing highly enhanced abundance in lemma, palea and glume tissues over leaf, stem, embryo, endosperm and root tissue were selected for further analysis. These wheat EST cluster sequences were used to identify rice cDNA homologs. The abundance of the rice cDNA homologs was compared in rice leaf and panicle (includes lemma and palea) cDNA libraries. Rice cDNAs showing preferential expression in the panicle were then used to identify homologous rice genomic DNA clones, the putative promoter sequences have been identified and cloned.

    摘要翻译： 在一系列由各种小麦组织制成的cDNA文库中，研究了小麦引物/外来cDNA文库中96个最丰富的EST簇序列的丰度。 选择30个cDNA序列，显示叶，茎，胚，胚乳和根组织中引物，内脏和叶片组织的丰度丰度，进一步分析。 这些小麦EST簇序列用于鉴定水稻cDNA同源物。 在水稻和穗（包括外来和外来）cDNA文库中比较了水稻cDNA同源物的丰度。 然后使用在穗中显示优先表达的水稻cDNA来鉴定同源的水稻基因组DNA克隆，推定的启动子序列已被鉴定和克隆。