公开(公告)号：US20080176267A1

公开(公告)日：2008-07-24

申请号：US11910541

申请日：2006-04-05

申请人： Ralf Hass ,  Hartmut Kueppers ,  Axel Dehn ,  Jutta Rogoll

发明人： Ralf Hass ,  Hartmut Kueppers ,  Axel Dehn ,  Jutta Rogoll

IPC分类号： C12Q1/02 ,  C12N5/02 ,  C12N5/00

CPC分类号： C12N5/0693 ,  C12N2503/00 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/502 ,  G01N33/5044 ,  G01N33/5088

摘要： The present invention relates to methods for obtaining primary-culture tumour cells from tumour tissue. More precisely, the present invention relates to a method for obtaining primary-culture tumour cells from tumour tissue, in particular breast carcinoma, where, in one method step, the tumour tissue is divided into tumour-tissue sections of a certain size, and these tissue sections are then cultured under defined conditions. The invention also concerns the primary-culture tumour cells that can be obtained by this method from the tumour tissue in particular primary-culture tumour cells from breast carcinoma Finally, the invention relates to the use of these primary-culture tumour cells for, among other things, determining an individual course of tumour treatment, or for testing and screening of new therapeutic agents against tumours.

摘要翻译： 本发明涉及从肿瘤组织获得原代培养肿瘤细胞的方法。 更准确地说，本发明涉及从肿瘤组织特别是乳腺癌获得原代培养肿瘤细胞的方法，其中在一个方法步骤中，将肿瘤组织分成一定大小的肿瘤组织切片，这些 然后在限定的条件下培养组织切片。 本发明还涉及可以通过该方法从肿瘤组织特别是来自乳腺癌的原代培养肿瘤细胞获得的原代培养肿瘤细胞。最后，本发明涉及这些原代培养肿瘤细胞在其他方面的用途 确定肿瘤治疗的单独疗程，或用于测试和筛选新的肿瘤治疗剂。