公开(公告)号：US5977297A

公开(公告)日：1999-11-02

申请号：US992676

申请日：1997-12-17

申请人： Rainer Obermeier ,  Jurgen Ludwig ,  Walter Sabel

发明人： Rainer Obermeier ,  Jurgen Ludwig ,  Walter Sabel

IPC分类号： C07K1/18 ,  C07K14/62 ,  A61K38/28 ,  A61K38/00 ,  C07K5/00 ,  C07K7/00

CPC分类号： C07K14/62

摘要： A process for isolating insulin which entails performing high-pressure liquid chromatography with pressure-stable acidic cation exchange material under a pressure of from 1.1 MPa to 40 MPa.

摘要翻译： 一种分离胰岛素的方法，其需要在压力为1.1MPa至40MPa的压力下用压力稳定的酸性阳离子交换材料进行高压液相色谱。

公开(公告)号：US5175471A

公开(公告)日：1992-12-29

申请号：US734611

申请日：1991-07-23

申请人： Gottfried Stockinger ,  Jurgen Ludwig

发明人： Gottfried Stockinger ,  Jurgen Ludwig

IPC分类号： H05B41/18 ,  H05B41/298 ,  H05B41/392

CPC分类号： H05B41/18 ,  H05B41/2988 ,  H05B41/3924 ,  Y10S315/05

摘要： A circuit arrangement for the operation of a fluorescent lamp which is connectable to an alternating-current power supply, for example, the elctrical power supply on board an aircraft, including a switch which is located between the lamp electrodes, and which is actuatable through the intermediary of a control circuit whereby the lamp electrodes are heated when the switch is closed. A measuring element which is connected to the input of the control circuit determines the decreasing voltage at least at one of the lamp electrodes, and the control circuit will open the switch for the ignition of the fluorescent lamp only when the determined voltage is essentially constant, or has reached a threshold value.

摘要翻译： 一种用于操作荧光灯的电路装置，其可连接到交流电源，例如飞机上的电动电源，包括位于灯电极之间的开关，并且其可通过 控制电路的中间体，由此当开关闭合时灯电极被加热。 连接到控制电路的输入的测量元件至少在一个灯电极上确定降低的电压，并且仅当所确定的电压基本上恒定时，控制电路将打开用于点燃荧光灯的开关， 或已经达到阈值。

公开(公告)号：US5674838A

公开(公告)日：1997-10-07

申请号：US385551

申请日：1995-02-08

申请人： Rainer Obermeier ,  Jurgen Ludwig ,  Dominique Tripier ,  Max Hropot

发明人： Rainer Obermeier ,  Jurgen Ludwig ,  Dominique Tripier ,  Max Hropot

IPC分类号： C12P21/02 ,  A61K38/00 ,  A61K38/55 ,  A61K47/48 ,  A61P7/02 ,  C07K1/107 ,  C07K1/12 ,  C07K14/815 ,  A61K38/16 ,  A61K38/58

CPC分类号： A61K47/48215 ,  C07K14/815 ,  A61K38/00

摘要： Hirudin derivatives can be prepared by the selective enzymic replacement of a part of the molecule with an amino derivative. The hirudin derivatives are suitable for preparing pharmaceuticals having an anticoagulatory effect.

摘要翻译： 水蛭素衍生物可以通过用氨基衍生物选择性酶取代一部分分子来制备。 水蛭素衍生物适用于制备具有抗凝作用的药物。

公开(公告)号：US5663291A

公开(公告)日：1997-09-02

申请号：US389487

申请日：1995-02-16

申请人： Rainer Obermeier ,  Martin Gerl ,  Jurgen Ludwig ,  Walter Sabel

发明人： Rainer Obermeier ,  Martin Gerl ,  Jurgen Ludwig ,  Walter Sabel

IPC分类号： C12P21/00 ,  C07K1/08 ,  C07K1/113 ,  C07K1/20 ,  C07K14/575 ,  C07K14/62 ,  C07K1/107 ,  C07K14/00 ,  C12P27/06

CPC分类号： C07K1/1133 ,  C07K14/62 ,  C07K2319/00

摘要： A process for obtaining insulin having correctly linked cystine bridges from a corresponding proinsulin amino acid chain in the presence of mercaptan, chaotropic auxiliaries and at a pH of 10 to 11 is described, as well as the direct conversion of the proinsulin to insulin with the aid of enzymes and direct isolation of the insulin from the reaction mixture with the aid of hydrophobic adsorber resins.

摘要翻译： 描述了在硫醇，离液序列助剂和10至11的pH值下，从相应的胰岛素原氨基酸链中获得具有正确连接的胱氨酸桥的胰岛素的方法，以及辅助的胰岛素原与胰岛素的直接转化 的酶，并借助疏水吸附树脂从反应混合物中直接分离胰岛素。

公开(公告)号：US4677192A

公开(公告)日：1987-06-30

申请号：US804325

申请日：1985-12-04

申请人： Rainer Obermeier ,  Volker Teetz ,  Jurgen Ludwig

发明人： Rainer Obermeier ,  Volker Teetz ,  Jurgen Ludwig

IPC分类号： A61K38/28 ,  C07G15/00 ,  C07K1/14 ,  C07K1/16 ,  C07K1/20 ,  C07K14/575 ,  C07K14/62 ,  C07K14/625 ,  A61K37/26 ,  C07K7/40

CPC分类号： C07K14/625

摘要： A method for separating insulin or certain insulin derivatives from mixtures containing the same by chromatography of the mixture on a column filled with silica gel having a particle size between 40 and 500 microns with an eluant mixture comprising chloroform, methanol, water and triethylamine.

摘要翻译： 一种通过色谱法分离胰岛素或某些胰岛素衍生物的方法，该混合物在填充有40-500微米粒度的硅胶柱上，用含有氯仿，甲醇，水和三乙胺的洗脱剂混合物进行色谱分离。

公开(公告)号：US3554873A

公开(公告)日：1971-01-12

申请号：US3554873D

申请日：1967-10-11

申请人： GUNTER LUTHER ,  JURGEN LUDWIG ,  MARTIN SCHULZE

发明人： LUTHER GUNTER ,  LUDWIG JURGEN ,  SCHULZE MARTIN

IPC分类号： B01D3/40 ,  C07C1/00 ,  C07C7/08 ,  C07C15/02 ,  C07C67/00 ,  B01D3/34 ,  C07C7/00

CPC分类号： B01D3/40 ,  C07C7/08 ,  C07C15/00

摘要： AN EXTRACTIVE DISTILLATION PROCESS FOR SIMULTANEOUSLY OBTAINING AROMATICS WITH DIFFERENT BOILING-POINT RANGES FROM A FEED STOCK CONTAINING VARIED AMOUNTS OF NONAROMATICS ALONG WITH THE DESIRED AROMATICS, SAID PROCESS BEING OF THE TYPE WHEREIN THE FEED STOCK IS PASSED THROUGH AT LEAST ONE EXTRACTIVE DISTILLATION COLUMN AND IS CONTACTED WITH A SELECTIVELY ACTIVE EXTRACTION AGENT TO WHICH AROMATICS OTHER THAN THE DESIRED AROMATICS HAVE BEEN ADDED, AND WHEREIN THE MIXTURE OF AROMATICS AND EXTRACTION AGENT IS WITHDRAWN FROM THE BOTTOM OF THE COLUMN WHILE WITHDRAWING THE NON-AROMATICS IN VAPOR FORM FROM THE TOP OF THE COLUMN, THE IMPROVEMENT COMPRISING PASSING THE AROMATICS CONTAINING MASS RECEIVED FROM THE SAID EXTRACTIVE DISTILLATION COLUMN THROUGH A PLURALITY OF IN-SERIES CONNECTED COLUMN SYSTEMS, THE NUMBER OF SAID SYSTEMS CORRESPONDING TO THE NUMBER OF AROMATICS TO BE OBTAINED AND SUBJECTING THE MASS IN EACH OF SAID COLUMN SYSTEMS SUCCESSIVELY TO AN EXTRACTIVE DISTILLATION AND TO A NORMAL DISTILLATION AND RECOVERING FROM EACH SYSTEM THE DESIRED AROMATIC FRACTION.

公开(公告)号：US5473049A

公开(公告)日：1995-12-05

申请号：US160376

申请日：1993-12-01

申请人： Rainer Obermeier ,  Martin Gerl ,  Jurgen Ludwig ,  Walter Sabel

发明人： Rainer Obermeier ,  Martin Gerl ,  Jurgen Ludwig ,  Walter Sabel

IPC分类号： C07K14/575 ,  A61K38/28 ,  A61P3/08 ,  C07K1/113 ,  C07K1/20 ,  C07K14/62 ,  C07K19/00 ,  C12N15/00 ,  C12P21/02

CPC分类号： C07K14/62

摘要： A process is described for obtaining proinsulin possessing correctly linked cystine bridges from a corresponding proinsulin amino acid chain in the presence of mercaptan and chaotropic auxiliary agents and at a pH of 10 to 11, as well as its direct isolation from the reaction mixture using hydrophobic adsorber resins.

摘要翻译： 描述了一种在硫醇和离液序列助剂存在下从相应的胰岛素原氨基酸链获得具有正确连接的胱氨酸桥的胰岛素原的过程，并且在10至11的pH下，以及使用疏水吸附剂直接从反应混合物中分离 树脂。

公开(公告)号：US4681931A

公开(公告)日：1987-07-21

申请号：US741865

申请日：1985-06-06

申请人： Rainer Obermeier ,  Ingeborg Salomon ,  Jurgen Ludwig

发明人： Rainer Obermeier ,  Ingeborg Salomon ,  Jurgen Ludwig

IPC分类号： C12N15/09 ,  A61K38/21 ,  C07K1/22 ,  C07K14/52 ,  C07K14/555 ,  C07K14/56 ,  C07K16/00 ,  C12P21/00 ,  C07K15/26 ,  A61K45/02

CPC分类号： C07K14/56

摘要： The invention relates to a process for the isolation and purification of .alpha.-interferons generated by plasmids from bacterial cultures which have been modified by genetic engineering, and of .alpha.-interferons from culture supernatants of induced mammalian cells, which comprises partition of appropriate crude substances containing interferon between an aqueous urea solution, which contains a surfactant, and a mixture of n-butanol and glacial acetic acid which contains water, and isolation of the purified .alpha.-interferons from the upper phase.

摘要翻译： 本发明涉及用于分离和纯化由经遗传工程修饰的细菌培养物的质粒产生的α-干扰素和由诱导的哺乳动物细胞的培养物上清液产生的α-干扰素的方法，该方法包括将含有 在含有表面活性剂的尿素水溶液和含有水的正丁醇和冰醋酸的混合物之间的干扰素，以及从上部相分离纯化的α-干扰素。

公开(公告)号：US4601852A

公开(公告)日：1986-07-22

申请号：US641357

申请日：1984-08-15

申请人： Rainer Obermeier ,  Jurgen Ludwig ,  Gerhard Seipke

发明人： Rainer Obermeier ,  Jurgen Ludwig ,  Gerhard Seipke

IPC分类号： C12P21/02 ,  A61K38/28 ,  C07K1/12 ,  C07K14/62 ,  C07K7/40

CPC分类号： C07K14/62

摘要： What is disclosed is a method for making human insulin or modified human insulin from pig insulin or modified pig insulin by reacting the pig insulin starting material at a pH below its isoelectric point with an excess of a threonine ester in the presence of trypsin or a trypsin-like enzyme.

摘要翻译： 公开的是通过在胰蛋白酶或胰蛋白酶存在下，使猪胰岛素起始物质在低于其等电点的pH与过量的苏氨酸酯反应从猪胰岛素或修饰的猪胰岛素制备人胰岛素或修饰的人胰岛素的方法 类酶。