公开(公告)号：US07208322B2

公开(公告)日：2007-04-24

申请号：US10116013

申请日：2002-04-02

Applicant: Mark L. Stolowitz ,  Jean P. Wiley ,  Guisheng Li ,  Kevin Lund

Inventor： Mark L. Stolowitz ,  Jean P. Wiley ,  Guisheng Li ,  Kevin Lund

IPC: G01N33/553 ,  G01N27/00 ,  G01N33/567 ,  G01N33/566

CPC classification number: G01N33/54373 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/553 ,  G01N33/54353 ,  G01N33/553 ,  G01N2610/00 ,  Y10S436/806 ,  Y10S436/829 ,  Y10T436/143333

Abstract: The present invention provides a sensor surface comprising: a substrate coated with a free electron metal; and a matrix layer disposed on the free electron metal, wherein the matrix layer comprises an organic compound having a boronic acid complexing moiety. The matrix is preferably a self-assembled monolayer (SAM), a mixed self-assembled monolayer (mSAM), or combinations thereof.

Abstract translation: 本发明提供了一种传感器表面，包括：涂覆有自由电子金属的基底; 以及设置在所述自由电子金属上的基质层，其中所述基质层包含具有硼酸络合部分的有机化合物。 基质优选为自组装单层（SAM），混合自组装单层（mSAM）或其组合。

公开(公告)号：US06630577B2

公开(公告)日：2003-10-07

申请号：US09874633

申请日：2001-06-04

Applicant: Mark L. Stolowitz ,  Guisheng Li ,  Jean P. Wiley

Inventor： Mark L. Stolowitz ,  Guisheng Li ,  Jean P. Wiley

IPC: C07F502

CPC classification number: C07J1/00 ,  C07C229/38 ,  C07C233/49 ,  C07C233/51 ,  C07C237/12 ,  C07C237/22 ,  C07C255/13 ,  C07C259/10 ,  C07C271/22 ,  C07C281/02 ,  C07D207/452 ,  C07D207/46 ,  C07D213/71 ,  C07F5/025 ,  G01N33/531 ,  G01N33/54353 ,  Y10S530/81 ,  Y10S530/816

Abstract: A reagent having the general formula of General Formula I: wherein group Z comprises a spacer selected from an aliphatic chain up to about 6 carbon equivalents in length, an unbranched aliphatic chain of from about 6 to 18 carbon equivalents in length with at least one of an intermediate amide and a disulfide moiety, and a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length; wherein group R is an electrophilic or nucleophilic moiety suitable for reaction of the reagent with a biologically active species; and wherein group Q is one of nothing at all, an amide, a methyl amide, a methylene, an ether, a thioether, a methyl ether, and a methyl thioether moiety. Also, a conjugate, a bioconjugate and a method of conjugating.

Abstract translation: 具有通式I的通式的试剂：其中基团Z包括选自长度为约6碳当量长度的脂肪族链的间隔基，长度为约6至18个碳当量长度的无支链脂族链，其中至少一个为 中间体酰胺和二硫化物部分，和长度为约3至12个碳当量的聚乙二醇链; 其中基团R是适于试剂与生物活性物质的反应的亲电或亲核部分; 并且其中基团Q是任何一个，酰胺，甲基酰胺，亚甲基，醚，硫醚，甲基醚和甲基硫醚部分。 另外，缀合物，生物缀合物和缀合方法。

公开(公告)号：US07179659B2

公开(公告)日：2007-02-20

申请号：US10115721

申请日：2002-04-02

Applicant: Mark L. Stolowitz ,  Jean P. Wiley ,  Guisheng Li ,  Kevin Lund

Inventor： Mark L. Stolowitz ,  Jean P. Wiley ,  Guisheng Li ,  Kevin Lund

IPC: G01N33/553 ,  G01N27/00 ,  G01N33/567 ,  G01N33/566

CPC classification number: G01N33/54373 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/553 ,  G01N33/54353 ,  G01N33/553 ,  G01N2610/00 ,  Y10S436/806 ,  Y10S436/829 ,  Y10T436/143333

Abstract: The present invention provides a method for detecting an analyte, comprising providing a sensor comprising a substrate coated with a free electron metal, a matrix layer disposed on the free electron metal, the matrix layer comprising an organic compound, wherein the organic compound has a boronic acid complexing moiety and, a boronic acid moiety complexed to the boronic acid complexing moiety; and contacting the sensor with the analyte to elicit a response; and measuring the response thereby detecting the analyte.

Abstract translation: 本发明提供了一种用于检测分析物的方法，包括提供包括涂覆有自由电子金属的基底的传感器，设置在自由电子金属上的基质层，所述基质层包含有机化合物，其中所述有机化合物具有硼 酸络合部分和与硼酸络合部分复合的硼酸部分; 并将传感器与分析物接触以引起响应; 并测量响应，从而检测分析物。