公开(公告)号：US20100112673A1

公开(公告)日：2010-05-06

申请号：US12524713

申请日：2007-05-15

Applicant: Pavel Kyslik ,  Vaclav Stepanek ,  Lenka Hollerova ,  Stanislav Becka ,  Vyasarayani Williams Rajasekar ,  Datla Anupama ,  Kamila Plhackova ,  Jaroslav Marsalek

Inventor： Pavel Kyslik ,  Vaclav Stepanek ,  Lenka Hollerova ,  Stanislav Becka ,  Vyasarayani Williams Rajasekar ,  Datla Anupama ,  Kamila Plhackova ,  Jaroslav Marsalek

IPC: C12N15/52 ,  C12N15/70 ,  C12N1/21

CPC classification number: C12N9/84 ,  C12Y305/01011

Abstract: The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from Achromobacter sp. CCM 4824 (formerly Comamonas testosteroni CCM 4824), eventually of the fragments of this sequence having the length of at least 150 nucleotides. The sequence can be used in the formation of a DNA construct, eventually the construct having at least one regulatory sequence regulating the expression of the gene and the production of a polypeptide with the penicillin acylase activity. The sequence can form part of a recombinant expression vector, which consists of the above-mentioned construct, promoter, translational start signal, translational and transcriptional stop signal. Further, the invention concerns a recombinant host cell, containing the nucleic acid construct carried by the vector or integrated into the cell chromosome, and the E. coli BL21 strain containing said sequence of the nucleotides encoding the penicillin acylase carried in the pKXIP1, the pKLP3 or the pKLP6 plasmid.

Abstract translation: 本发明包括具有（2646）bp大小的核苷酸序列，其中核苷酸的顺序与编码来自无色杆菌属的青霉素酰基转移酶的核苷酸序列的顺序相同。 CCM 4824（以前的Comamonas testosteroni CCM 4824），最终具有长度至少为150个核苷酸的该序列的片段。 该序列可以用于DNA构建体的形成，最终具有至少一个调节基因表达调节序列和具有青霉素酰基转移酶活性的多肽的产生的构建体。 该序列可以形成重组表达载体的一部分，其由上述构建体，启动子，翻译启动信号，翻译和转录停止信号组成。 此外，本发明涉及含有载体携带或整合到细胞染色体中的核酸构建体的重组宿主细胞，以及含有编码pKXIP1携带的青霉素酰基转移酶的核苷酸序列的大肠杆菌BL21菌株，pKLP3 或pKLP6质粒。

公开(公告)号：US08039604B2

公开(公告)日：2011-10-18

申请号：US12524713

申请日：2007-05-15

Applicant: Pavel Kyslik ,  Vaclav Stepanek ,  Lenka Hollerova ,  Stanislav Becka ,  Vyasarayani Williams Rajasekar ,  Datla Anupama ,  Kamila Plhackova ,  Jaroslav Marsalek

Inventor： Pavel Kyslik ,  Vaclav Stepanek ,  Lenka Hollerova ,  Stanislav Becka ,  Vyasarayani Williams Rajasekar ,  Datla Anupama ,  Kamila Plhackova ,  Jaroslav Marsalek

IPC: C07H21/02 ,  C07H21/04 ,  C12P1/00

CPC classification number: C12N9/84 ,  C12Y305/01011

Abstract: The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from Achromobacter sp. CCM 4824 (formerly Comamonas testosteroni CCM 4824), eventually of the fragments of this sequence having the length of at least 150 nucleotides. The sequence can be used in the formation of a DNA construct, eventually the construct having at least one regulatory sequence regulating the expression of the gene and the production of a polypeptide with the penicillin acylase activity. The sequence can form part of a recombinant expression vector, which consists of the above-mentioned construct, promoter, translational start signal, translational and transcriptional stop signal. Further, the invention concerns a recombinant host cell, containing the nucleic acid construct carried by the vector or integrated into the cell chromosome, and the E. coli BL21 strain containing said sequence of the nucleotides encoding the penicillin acylase carried in the pKXIP1, the pKLP3 or the pKLP6 plasmid.

Abstract translation: 本发明包括具有（2646）bp大小的核苷酸序列，其中核苷酸的顺序与编码来自无色杆菌属的青霉素酰基转移酶的核苷酸序列的顺序相同。 CCM 4824（以前的Comamonas testosteroni CCM 4824），最终具有长度至少为150个核苷酸的该序列的片段。 该序列可以用于DNA构建体的形成，最终具有至少一个调节基因表达调节序列和具有青霉素酰基转移酶活性的多肽的产生的构建体。 该序列可以形成重组表达载体的一部分，其由上述构建体，启动子，翻译启动信号，翻译和转录停止信号组成。 此外，本发明涉及含有载体携带或整合到细胞染色体中的核酸构建体的重组宿主细胞，以及含有编码pKXIP1携带的青霉素酰基转移酶的核苷酸序列的大肠杆菌BL21菌株，pKLP3 或pKLP6质粒。