公开(公告)号：US09624467B2

公开(公告)日：2017-04-18

申请号：US14068954

申请日：2013-10-31

申请人： Dean Der-Syh Tzeng ,  Win-De Huang ,  Hsin-Chih Ko

发明人： Dean Der-Syh Tzeng ,  Win-De Huang ,  Hsin-Chih Ko

IPC分类号： C12N3/00 ,  A01N63/00 ,  A61K35/742 ,  C12N1/20

CPC分类号： C12N3/00 ,  A01N63/00 ,  A61K35/742 ,  C12N1/20

摘要： The present invention is a method for preparing a biocontrol formulation by the following steps. A Streptomyces bacterium is selected simultaneously with superior sporulation ability, chitinase activity and antagonistic effectiveness against plant fungal pathogens by culturing with fungal pathogens, wherein the selected Streptomyces bacterium is cultured on a chitin-limited plate to assess the ability of sporulation and chitinase activity, and the Streptomyces bacterium with superior sporulation ability is the strain with producing powder-like and maturity form spore chains. Spores from the selected bacterium is collected. The obtained spores are taken as a seed inoculum. Then the seed inoculum is cultured to directly yield a broth medium containing at least 109 viable spores/ml which the spores contained in the broth medium are well suspended. The biocontrol formulation is obtained. The present invention further provided a biocontrol formulation containing a high concentration of Streptomyces spp. spores.

公开(公告)号：US20140057336A1

公开(公告)日：2014-02-27

申请号：US14068954

申请日：2013-10-31

申请人： Dean Der-Syh Tzeng ,  Win-De Huang ,  Hsin-Chih Ko

发明人： Dean Der-Syh Tzeng ,  Win-De Huang ,  Hsin-Chih Ko

IPC分类号： C12N3/00

CPC分类号： C12N3/00 ,  A01N63/00 ,  A61K35/742 ,  C12N1/20

摘要： The present invention is a method for preparing a biocontrol formulation by the following steps. A Streptomyces bacterium is selected simultaneously with superior sporulation ability, chitinase activity and antagonistic effectiveness against plant fungal pathogens by culturing with fungal pathogens, wherein the selected Streptomyces bacterium is cultured on a chitin-limited plate to assess the ability of sporulation and chitinase activity, and the Streptomyces bacterium with superior sporulation ability is the strain with producing powder-like and maturity form spore chains. Spores from the selected bacterium is collected. The obtained spores are taken as a seed inoculum. Then the seed inoculum is cultured to directly yield a broth medium containing at least 109 viable spores/ml which the spores contained in the broth medium are well suspended. The biocontrol formulation is obtained. The present invention further provided a biocontrol formulation containing a high concentration of Streptomyces spp. spores.

摘要翻译： 本发明是通过以下步骤制备生物防治制剂的方法。 通过用真菌病原体培养，同时选择链霉菌属细菌，其具有优良的孢子形成能力，几丁质酶活性和对植物真菌病原体的拮抗作用，其中将选择的链霉菌细菌在几丁质限制板上培养以评估孢子形成和几丁质酶活性的能力， 具有优异孢子形成能力的链霉菌属细菌是产生粉末状和成熟形式孢子链的菌株。 收集所选细菌的孢子。 将获得的孢子作为种子接种物。 然后培养种子接种物以直接产生含有至少109个活孢子/ ml的培养基培养基，培养基培养基中所含的孢子充分悬浮。 获得生物防治制剂。 本发明还提供了含有高浓度链霉菌属（Streptomyces spp）的生物防治剂。 孢子

公开(公告)号：US20070148755A1

公开(公告)日：2007-06-28

申请号：US11708524

申请日：2007-02-20

申请人： Dean Tzeng ,  Win-De Huang ,  Hsin-Chih Ko

发明人： Dean Tzeng ,  Win-De Huang ,  Hsin-Chih Ko

IPC分类号： C12N1/20

CPC分类号： C12N3/00 ,  A01N63/00 ,  A61K35/742 ,  C12N1/20

摘要： The present invention is a method for preparing a biocontrol formulation by the following steps. A Streptomyces bacterium is selected simultaneously with superior sporulation ability, chitinase activity and antagonistic effectiveness against plant fungal pathogens by culturing with fungal pathogens, wherein the selected Streptomyces bacterium is cultured on a chitin-limited plate to assess the ability of sporulation and chitinase activity, and the Streptomyces bacterium with superior sporulation ability is the strain with producing powder-like and maturity form spore chains. Spores from the selected bacterium is collected. The obtained spores are taken as a seed inoculum. Then the seed inoculum is cultured to directly yield a broth medium containing at least 109 viable spores/ml which the spores contained in the broth medium are well suspended. The biocontrol formulation is obtained. The present invention further provided a biocontrol formulation containing a high concentration of Streptomyces spp. spores.

摘要翻译： 本发明是通过以下步骤制备生物防治制剂的方法。 通过用真菌病原体培养，同时选择链霉菌属细菌，其具有优良的孢子形成能力，几丁质酶活性和对植物真菌病原体的拮抗作用，其中将选择的链霉菌细菌在几丁质限制板上培养以评估孢子形成和几丁质酶活性的能力， 具有优异孢子形成能力的链霉菌属细菌是产生粉末状和成熟形式孢子链的菌株。 收集所选细菌的孢子。 将获得的孢子作为种子接种物。 然后培养种子接种物以直接产生含有至少10个活孢子/ ml的肉汤培养基，培养基中含有的孢子充分悬浮。 获得生物防治制剂。 本发明还提供了含有高浓度链霉菌属（Streptomyces spp）的生物防治剂。 孢子

公开(公告)号：US20060257373A1

公开(公告)日：2006-11-16

申请号：US11125571

申请日：2005-05-10

申请人： Dean Tzeng ,  Win-De Huang ,  Hsin-Chih Ko

发明人： Dean Tzeng ,  Win-De Huang ,  Hsin-Chih Ko

IPC分类号： A61K35/74 ,  A01N63/00 ,  C12N1/20

CPC分类号： C12N1/20 ,  A01N63/00 ,  A61K35/742 ,  C12N3/00

摘要： The present invention is a method for preparing a antifungal formulation containing a high concentration of spores comprising providing Streptomyces bacteria with sporulation competence; propagating the bacterial strain for obtaining spores under an appropriate condition, collecting the spores, preparing the spores to obtain a small scale of the spore biomass as a seed inoculum, and culturing the seed inoculum at 28 to 37° C., 80 to 250 rpm, 0.25 to 0.75 vvm and pH at 5.0 to 8.0 to obtain a large scale fermentation of the bacteria in a suitable broth medium. The present invention further comprises a antifungal formulation containing Streptomyces spp. comprising a high concentration of spores and a liquid medium for culturing spore biomass of Streptomyces spp.

摘要翻译： 本发明是一种制备含有高浓度孢子的抗真菌制剂的方法，包括提供具有孢子形成能力的链霉菌; 在适当的条件下繁殖细菌菌株以获得孢子，收集孢子，制备孢子以获得小规模的作为种子接种物的孢子生物质，并在28至37℃，80至250rpm下培养种子接种物 ，0.25至0.75vvm，pH为5.0至8.0，以在合适的培养基中获得细菌的大规模发酵。 本发明还包含含有链霉菌属（Streptomyces spp。）的抗真菌制剂。 包括高浓度的孢子和用于培养链霉菌的孢子生物量的液体培养基。