公开(公告)号：US20220220162A1

公开(公告)日：2022-07-14

申请号：US17148227

申请日：2021-01-13

申请人： Houshang Alizadeh ,  Fariba Rafiei ,  Ali-Akbar Shahnejat Bushehri

发明人： Houshang Alizadeh ,  Fariba Rafiei ,  Ali-Akbar Shahnejat Bushehri

IPC分类号： C07K14/415 ,  C12N9/24 ,  C12N9/64 ,  C12N9/96 ,  A61P35/00 ,  C07K16/16

摘要： A targeted delivery of recombinant therapeutic protein to inhibit the growth of cancer cells is disclosed. The recombinant protein derived from Abrin-a A-chain able to inhibit the growth of cancer cell and conjugated to stxB to form Abrin-f-stxB. The abrin toxin A chain and the Shiga toxin B chain are linked with the furin linker to form a recombinant therapeutic protein as chemotherapeutic agent to inhibit the growth of cancer cells. A conjugate for the delivery of a compound into cancer cells, comprising a first module mediates cell targeting and facilitates cellular uptake, a second module facilitates transport to the endoplasmic reticulum (ER), a third module mediates translocation from the ER to the cytosol and a compound that is desired to be delivered to the cytosol. The recombinant protein Abrin-f-stxB exhibits high binding affinity of stxB as a drug delivery system to Gb3 receptor in targeted cancer diagnosis and treatment.