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    Production of therapeutic proteins in genetically modified mammalian cells
    2.
    发明授权
    Production of therapeutic proteins in genetically modified mammalian cells 有权

    公开(公告)号：US10030063B2

    公开(公告)日：2018-07-24

    申请号：US14651694

    申请日：2013-12-16

    申请人： Mara Fornaro ,  David Jonathan Glass ,  Thomas Jostock ,  Holger Laux ,  Sandrine Romand

    发明人： Mara Fornaro ,  David Jonathan Glass ,  Thomas Jostock ,  Holger Laux ,  Sandrine Romand

    IPC分类号： C07K14/65 ,  C12N15/113 ,  C12P21/02 ,  C12N15/11

    摘要： The invention relates to methods for the production of therapeutic proteins in mammalian cells. In one embodiment, the method comprises producing a therapeutic protein such as IGF-1 in a mammalian cell endogenously expressing a cognate receptor of said recombinant therapeutic protein and wherein binding of said therapeutic protein to said cognate receptor results in a low titer of the therapeutic protein, the method comprising with a mammalian cell being deficient in the expression of the cognate receptor of said therapeutic protein and being transformed with an expression vector comprising a nucleic acid molecule encoding the therapeutic protein: a. Cultivating said cell under conditions allowing the expression of the therapeutic protein; and b. Harvesting the therapeutic protein from the mammalian cell cultivated in step a, wherein said mammalian cell produces at least 1.5 fold more therapeutic protein than a cell in which the expression of the cognate receptor has not been so modified.

    Air conditioning system
    3.
    发明授权
    Air conditioning system 有权

    公开(公告)号：US11772456B2

    公开(公告)日：2023-10-03

    申请号：US17128000

    申请日：2020-12-18

    申请人： Holger Laux ,  Thorsten Moellert ,  Matthias Schall

    发明人： Holger Laux ,  Thorsten Moellert ,  Matthias Schall

    IPC分类号： B60H1/22 ,  B60H1/00

    CPC分类号： B60H1/2225 ,  B60H1/00028 ,  B60H1/00564 ,  B60H2001/00128

    摘要： An air-conditioning system for air-conditioning a vehicle interior is disclosed. The air-conditioning system includes a blower for generating an air flow, a heating device for heating the air flow, and a ducting system that divides the air flow downstream of the heating device over at least two part air flows and supplies the at least two part air flows via associated ducts to associated air outlets, through which the part air flows exit into the vehicle interior. The heating device includes at least two separate regions each with at least one electric heating element that is separately controllable via a control device. The at least two separate regions are each permanently assigned one of the associated ducts of the ducting system.

    AIR CONDITIONING SYSTEM
    4.
    发明申请
    AIR CONDITIONING SYSTEM 有权

    公开(公告)号：US20210188044A1

    公开(公告)日：2021-06-24

    申请号：US17128000

    申请日：2020-12-18

    申请人： Holger Laux ,  Thorsten Moellert ,  Matthias Schall

    发明人： Holger Laux ,  Thorsten Moellert ,  Matthias Schall

    IPC分类号： B60H1/22 ,  B60H1/00

    摘要： An air-conditioning system for air-conditioning a vehicle interior is disclosed. The air-conditioning system includes a blower for generating an air flow, a heating device for heating the air flow, and a ducting system that divides the air flow downstream of the heating device over at least two part air flows and supplies the at least two part air flows via associated ducts to associated air outlets, through which the part air flows exit into the vehicle interior. The heating device includes at least two separate regions each with at least one electric heating element that is separately controllable via a control device. The at least two separate regions are each permanently assigned one of the associated ducts of the ducting system.

    NOVEL EUKARYOTIC CELLS AND METHODS FOR RECOMBINANTLY EXPRESSING A PRODUCT OF INTEREST
    6.
    发明申请
    NOVEL EUKARYOTIC CELLS AND METHODS FOR RECOMBINANTLY EXPRESSING A PRODUCT OF INTEREST 审中-公开
    新兴的真菌细胞和方法来重新表达兴趣产品

    公开(公告)号：US20170002059A1

    公开(公告)日：2017-01-05

    申请号：US15104580

    申请日：2014-12-18

    申请人： Thomas JOSTOCK ,  Holger LAUX ,  Anett RITTER

    发明人： Thomas JOSTOCK ,  Holger LAUX ,  Anett RITTER

    IPC分类号： C07K16/00 ,  C12N15/113

    CPC分类号： C07K16/00 ,  C07K2317/14 ,  C12N15/113 ,  C12N2310/14 ,  C12N2510/02 ,  C12N2511/00

    摘要： The disclosure pertains to novel eukaryotic cell suitable for recombinant production of a product of interest, wherein the effect of the expression product of an endogenous gene C12orf35 is impaired in said cell, thereby increasing their productivity when recombinantly expressing a polypeptide of interest. Furthermore, the present disclosure provides associated technologies wherein such host cells are used in recombinant production technologies.

    摘要翻译： 本公开涉及适用于重组产生感兴趣的产物的新型真核细胞，其中所述细胞内的内源基因C12orf35的表达产物的作用受损，从而在重组表达感兴趣的多肽时增加其生产力。 此外，本公开提供了相关技术，其中这种宿主细胞用于重组生产技术。

    PRODUCTION OF THERAPEUTIC PROTEINS IN GENETICALLY MODIFIED MAMMALIAN CELLS
    8.
    发明申请
    PRODUCTION OF THERAPEUTIC PROTEINS IN GENETICALLY MODIFIED MAMMALIAN CELLS 审中-公开
    在遗传修饰的哺乳动物细胞中生产治疗蛋白

    公开(公告)号：US20150322131A1

    公开(公告)日：2015-11-12

    申请号：US14651694

    申请日：2013-12-16

    申请人： Mara FORNARO ,  David Jonathan GLASS ,  Thomas JOSTOCK ,  Holger LAUX ,  Sandrine ROMAND

    发明人： Mara FORNARO ,  David Jonathan GLASS ,  Thomas JOSTOCK ,  Holger LAUX ,  Sandrine ROMAND

    IPC分类号： C07K14/65 ,  C12N15/113

    CPC分类号： C07K14/65 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1138 ,  C12N2310/14 ,  C12N2310/531 ,  C12P21/02

    摘要： The invention relates to methods for the production of therapeutic proteins in mammalian cells. In one embodiment, the method comprises producing a therapeutic protein such as IGF-1 in a mammalian cell endogenously expressing a cognate receptor of said recombinant therapeutic protein and wherein binding of said therapeutic protein to said cognate receptor results in a low titer of the therapeutic protein, the method comprising with a mammalian cell being deficient in the expression of the cognate receptor of said therapeutic protein and being transformed with an expression vector comprising a nucleic acid molecule encoding the therapeutic protein: a. Cultivating said cell under conditions allowing the expression of the therapeutic protein; and b. Harvesting the therapeutic protein from the mammalian cell cultivated in step a, wherein said mammalian cell produces at least 1.5 fold more therapeutic protein than a cell in which the expression of the cognate receptor has not been so modified.

    摘要翻译： 本发明涉及在哺乳动物细胞中产生治疗性蛋白质的方法。 在一个实施方案中，该方法包括在内源性表达所述重组治疗性蛋白质的同源受体的哺乳动物细胞中产生诸如IGF-1的治疗性蛋白质，并且其中所述治疗性蛋白质与所述同源受体的结合导致治疗性蛋白质的低滴度 所述方法包括哺乳动物细胞缺乏所述治疗性蛋白质的同源受体的表达，并用包含编码治疗性蛋白质的核酸分子的表达载体转化：a。 在允许治疗性蛋白质表达的条件下培养所述细胞; 和b。 从步骤a中培养的哺乳动物细胞收获治疗性蛋白质，其中所述哺乳动物细胞产生比同源受体的表达未被如此修饰的细胞至少1.5倍的治疗性蛋白质。