公开(公告)号：US20130342837A1

公开(公告)日：2013-12-26

申请号：US13968744

申请日：2013-08-16

申请人： Handyem inc.

发明人： Alain Chandonnet ,  Michel Fortin ,  Dany Nolet

IPC分类号： G01N15/14

CPC分类号： G01N15/1436 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006

摘要： The present disclosure relates to the field characterization of particles in a sample solution. More specifically, the present disclosure relates to a flow cell and a method for characterizing particles by means of collected non-Gaussian temporal signals. The present flow cell and method rely on an excitation fiber with a channel. The excitation fiber has a core for transporting an excitation light generated by a light source, and defines a channel through a portion of its core. The channel of the excitation fiber directs a flow of the sample solution. The excitation fiber, the channel and collection fibers characteristics are selected, proportioned and positioned to generate collected light with a non-Gaussian temporal intensity profile.

摘要翻译： 本公开涉及样品溶液中颗粒的场表征。 更具体地，本公开涉及通过收集的非高斯时间信号来表征粒子的流动池和方法。 本流动池和方法依赖于具有通道的激发光纤。 激发光纤具有用于传输由光源产生的激发光的芯，并且通过其芯的一部分限定通道。 激发光纤的通道引导样品溶液的流动。 激发光纤，通道和收集光纤特性被选择，成比例和定位，以产生具有非高斯时间强度分布的收集光。

公开(公告)号：US20160320288A1

公开(公告)日：2016-11-03

申请号：US15106056

申请日：2013-12-18

申请人： HANDYEM INC.

发明人： Michel FORTIN ,  Alain CHANDONNET ,  Dany NOLET

IPC分类号： G01N15/14 ,  G01N21/64 ,  G01N21/05

CPC分类号： G01N15/1436 ,  G01N15/1459 ,  G01N21/05 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/645 ,  G01N2015/1006 ,  G01N2021/4707 ,  G01N2021/6439 ,  G01N2021/6482 ,  G01N2201/08

摘要： The present relates to a chip assembly, a flow cell and a cytometer for characterizing particles in a sample solution. The chip assembly comprises a pair of chips, at least one of the chip defining on its inner surface at least two channels, the two channels defining therebetween a common intersecting area. Each channel is adapted for receiving one or more optical fibers. The chips define a through-hole extending throughout the chip assembly in a transverse direction relative to the channels and passing through the common intersecting area. The flow cell comprises the chip assembly, an excitation fiber and at least one collection fiber extending through respective channels; the collection fiber for collecting light scattered or emitted by particles flowing through the through-hole and excited by an excitation light transported by the excitation fiber. The flow cytometer comprises a light source for generating the excitation light and the flow cell.

摘要翻译： 本发明涉及用于表征样品溶液中的颗粒的芯片组件，流动池和细胞仪。 芯片组件包括一对芯片，芯片中的至少一个在其内表面上限定至少两个通道，两个通道在其间限定共同的相交区域。 每个通道适于接收一个或多个光纤。 芯片限定穿过芯片组件的通孔相对于通道在横向上延伸并且穿过公共交叉区域。 流动池包括芯片组件，激励光纤和延伸穿过相应通道的至少一个收集光纤; 该收集纤维用于收集由流过该通孔并由激发光纤传送的激发光激发的颗粒散射或发射的光。 流式细胞仪包括用于产生激发光的光源和流动池。

公开(公告)号：US10359354B2

公开(公告)日：2019-07-23

申请号：US15106056

申请日：2013-12-18

申请人： HANDYEM INC.

发明人： Michel Fortin ,  Alain Chandonnet ,  Dany Nolet

IPC分类号： G01N15/14 ,  G01N21/64 ,  G01N21/05 ,  G01N21/53 ,  G01N21/47 ,  G01N15/10

摘要： The present relates to a chip assembly, a flow cell and a cytometer for characterizing particles in a sample solution. The chip assembly comprises a pair of chips, at least one of the chip defining on its inner surface at least two channels, the two channels defining therebetween a common intersecting area. Each channel is adapted for receiving one or more optical fibers. The chips define a through-hole extending throughout the chip assembly in a transverse direction relative to the channels and passing through the common intersecting area. The flow cell comprises the chip assembly, an excitation fiber and at least one collection fiber extending through respective channels; the collection fiber for collecting light scattered or emitted by particles flowing through the through-hole and excited by an excitation light transported by the excitation fiber. The flow cytometer comprises a light source for generating the excitation light and the flow cell.

公开(公告)号：US08736837B2

公开(公告)日：2014-05-27

申请号：US13968744

申请日：2013-08-16

申请人： Handyem inc.

发明人： Dany Nolet ,  Alain Chandonnet ,  Michel Fortin

IPC分类号： G01N21/00

CPC分类号： G01N15/1436 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006

摘要： The present disclosure relates to the field characterization of particles in a sample solution. More specifically, the present disclosure relates to a flow cell and a method for characterizing particles by means of collected non-Gaussian temporal signals. The present flow cell and method rely on an excitation fiber with a channel. The excitation fiber has a core for transporting an excitation light generated by a light source, and defines a channel through a portion of its core. The channel of the excitation fiber directs a flow of the sample solution. The excitation fiber, the channel and collection fibers characteristics are selected, proportioned and positioned to generate collected light with a non-Gaussian temporal intensity profile.

摘要翻译： 本公开涉及样品溶液中颗粒的场表征。 更具体地，本公开涉及通过收集的非高斯时间信号来表征粒子的流动池和方法。 本流动池和方法依赖于具有通道的激发光纤。 激发光纤具有用于传输由光源产生的激发光的芯，并且通过其芯的一部分限定通道。 激发光纤的通道引导样品溶液的流动。 激发光纤，通道和收集光纤特性被选择，成比例和定位，以产生具有非高斯时间强度分布的收集光。