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公开(公告)号:US5980958A
公开(公告)日:1999-11-09
申请号:US91105
申请日:1998-06-12
Applicant: Thomas William Naylor , Trevor Williamson , Anthony Peter Joseph Trinci , Geoffrey David Robson , Marilyn Gail Wiebe
Inventor: Thomas William Naylor , Trevor Williamson , Anthony Peter Joseph Trinci , Geoffrey David Robson , Marilyn Gail Wiebe
IPC: A23J1/00 , A23J3/00 , A23J3/20 , A23L1/00 , A23L1/28 , C12N1/08 , C12N1/14 , C12N15/01 , C12R1/77 , A23J1/18
Abstract: A human food product comprises a higher ploidy strain of Fungi imperfecti of which each parent has a genetic growth constraint not shared by the other parent. One parent is for example auxotrophic for two compounds selected from histidine, arginine, leucine and adenine and the other for the other two. The nucleic acid content of the strain is preferably less than 2% by weight. The strain may be made by killing a higher ploidy strain of Fungi imperfecti and simultaneously reducing its nucleic acid content to below 2% by weight by treatment with water. The process may include preparing a higher ploidy strain of edible Fusarium by culturing two auxotrophs thereof together in a medium containing nutrients permitting both to grow and then culturing organisms derived from such culture in minimal medium. The invention is applicable to the production of human food by culturing the strain in conditions such that reversion or partial reversion to the haploid form(s) is minimised.
Abstract translation: PCT No.PCT / GB96 / 03046 Sec。 371日期:1998年6月12日 102(e)1998年6月12日PCT PCT 1996年12月9日PCT公布。 出版物WO97 / 22686 日期1997年6月26日人类食品包括更高倍性的真菌菌株,其中每个亲本具有不由另一方父母共享的遗传增长限制。 一个亲本是例如两种选自组氨酸,精氨酸,亮氨酸和腺嘌呤的化合物的营养缺陷型,另一种是另外两种。 菌株的核酸含量优选小于2重量%。 可以通过杀死更高倍性的真菌菌株并同时通过用水处理将其核酸含量降低到低于2重量%来制备菌株。 该方法可以包括通过在包含营养物质的培养基中培养两种营养缺陷型营养素来制备较高倍性的可食镰刀菌菌株,所述培养基允许生长,然后在基本培养基中培养来自这种培养物的生物。 本发明适用于通过在使单倍体形式的逆转或部分逆转最小化的条件下培养菌株来生产人类食物。
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公开(公告)号:US5935841A
公开(公告)日:1999-08-10
申请号:US687571
申请日:1996-08-09
Applicant: Anthony Peter Joseph Trinci , Geoffrey David Robson , Marilyn Gail Wiebe , Thomas William Naylor , Trevor Williamson
Inventor: Anthony Peter Joseph Trinci , Geoffrey David Robson , Marilyn Gail Wiebe , Thomas William Naylor , Trevor Williamson
IPC: A23L1/30 , A01H15/00 , A23J1/18 , A23L1/03 , A23L1/28 , A23L1/314 , C12N1/14 , C12R1/77 , C12N1/00 , C12N1/02 , A23C9/12
CPC classification number: C12R1/77 , A01H15/00 , A23J1/18 , A23L13/424 , A23L29/065 , A23L31/00 , C12N1/14 , Y10S435/911
Abstract: Filamentous microorganisms of improved properties, for example growth rate, may be obtained by culturing a sample of desired morphology until a substantial proportion of the culture diverges from that morphology, selecting an organism from the culture at that stage which exhibits the desired morphology and repeating the process with the selected microorganism. Furthermore, an isolated Fusarium graminearum having all of the characteristics of IMI 366464 and also possessing a greater morphological stability and growth rate than Fusarium graminearium strain IMI 145,425 is disclosed.
Abstract translation: PCT No.PCT / GB95 / 00273 Sec。 371日期:1996年8月9日 102(e)日期1996年8月9日PCT提交1995年2月10日PCT公布。 公开号WO95 / 21910 日期1995年8月17日可以通过培养所需形态的样品获得改善的性质,例如生长速率的丝状微生物,直到大部分培养物从该形态发散出来,从该阶段的培养物中选择生物体, 所需的形态并用选定的微生物重复该过程。 此外,公开了具有IMI366464的所有特征并且还具有比禾谷镰刀菌菌株IMI 145,425更大的形态稳定性和生长速率的分离的禾谷镰孢镰刀菌。
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公开(公告)号:US06270816B1
公开(公告)日:2001-08-07
申请号:US09407961
申请日:1999-09-29
Applicant: Thomas William Naylor , Trevor Williamson , Anthony Peter Joseph Trinci , Geoffrey David Robson , Marilyn Gail Wiebe
Inventor: Thomas William Naylor , Trevor Williamson , Anthony Peter Joseph Trinci , Geoffrey David Robson , Marilyn Gail Wiebe
IPC: A23C912
Abstract: A human food product comprises a higher ploidy strain of Fungi imperfecti of which each parent has a genetic growth constraint not shared by the other parent. One parent is for example auxotrophic for two compounds selected from histidine, arginine, leucine and adenine and the other for the other two. The nucleic acid content of the strain is preferably less than 2% by weight. The strain may be made by killing a higher ploidy strain of Fungi imperfecti and simultaneously reducing its nucleic acid content to below 2% by weight by treatment with water. The process may include preparing a higher ploidy strain of edible Fusarium by culturing two auxotrophs thereof together in a medium containing nutrients permitting both to grow and then culturing organisms derived from such culture in minimal medium. The invention is applicable to the production of human food by culturing the strain in conditions such that reversion or partial reversion to the haploid form(s) is minimised.
Abstract translation: 人类食品包括更高倍性真菌菌株,其中每个亲本具有不由另一亲属共享的遗传生长约束。 一个亲本是例如两种选自组氨酸,精氨酸,亮氨酸和腺嘌呤的化合物的营养缺陷型,另一种是另外两种。 菌株的核酸含量优选小于2重量%。 可以通过杀死更高倍性的真菌菌株并同时通过用水处理将其核酸含量降低到低于2重量%来制备菌株。 该方法可以包括通过在包含营养物质的培养基中培养两种营养缺陷型营养素来制备较高倍性的可食镰刀菌菌株,所述培养基允许生长,然后在基本培养基中培养来自这种培养物的生物。 本发明适用于通过在使单倍体形式的逆转或部分逆转最小化的条件下培养菌株来生产人类食物。
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