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    Method for producing transgenic plants with modified 5-aminolevulinic acid biosynthesis, method for identifying 5-aminolevulinic acid synthesis effectors
    1.
    发明授权
    Method for producing transgenic plants with modified 5-aminolevulinic acid biosynthesis, method for identifying 5-aminolevulinic acid synthesis effectors 失效
    用修饰的5-氨基乙酰丙酸生物合成生产转基因植物的方法，用于鉴定5-氨基乙酰丙酸合成效应物的方法

    公开(公告)号：US06603062B1

    公开(公告)日：2003-08-05

    申请号：US09581105

    申请日：2000-08-31

    申请人： Frank Schmidt ,  Günter Donn

    发明人： Frank Schmidt ,  Günter Donn

    IPC分类号： A01H500

    CPC分类号： C12N9/1029 ,  C12N9/90 ,  C12N15/8243 ,  C12N15/8274

    摘要： Provided is a method for producing transgenic monocotyledonous plants, plant cells, plant parts, seeds, and reproduction material with modified 5-aminolevulinic acid biosynthesis. This is achieved by stably integrating one or several nucleic acid molecules coding for a protein with a 5-aminolevulinic acid synthase function (ALAS) isolated from the alpha group of purple bacteria, an active fragment thereof or an antisense or complementary sequence thereof, into tie plant genome in stable form. This method can also be used to control undesired vegetation Also provided is a method for producing transgenic plants or plant cells whose glutamate-1-semialdehyde transferase (GSAAT) expression is suppressed or inhibited by stable integration of at least one nucleic acid molecule encoding an ALAS isolated from the alpha group of purple bacteria into the plant plastome by plastid transformation.

    摘要翻译： 提供了具有经修饰的5-氨基乙酰丙酸生物合成的转基因单子叶植物，植物细胞，植物部分，种子和繁殖材料的方法。 这是通过将编码蛋白质的一种或多种核酸分子与从紫色细菌的α组，其活性片段或其反义或互补序列分离的5-氨基乙酰丙酸合酶功能（ALAS）稳定整合而获得的， 植物基因组稳定。 该方法也可以用于控制不期望的植物。还提供了一种用于产生转基因植物或植物细胞的方法，其中谷氨酸-1-半醛转移酶（GSAAT）表达被抑制或抑制，其至少一个编码ALAS的核酸分子的稳定整合 通过质体转化从紫色细菌的α组分离成植物塑料。

    Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation
    3.
    发明授权
    Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation 失效
    具有长期，高效植物再生能力的玉米（L.），包括具有异源基因的可育转基因玉米植物及其制备

    公开(公告)号：US06284945B1

    公开(公告)日：2001-09-04

    申请号：US08897736

    申请日：1997-07-21

    申请人： Denes Dudits ,  Sandor Morocz ,  Janos Nemeth ,  Günter Donn

    发明人： Denes Dudits ,  Sandor Morocz ,  Janos Nemeth ,  Günter Donn

    IPC分类号： A01H500

    CPC分类号： A01H5/10 ,  A01H4/00 ,  A01H4/005 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8206 ,  C12N15/8277

    摘要： Protoplasts which regenerate reproducibly in a short time to normal, fertile plants can be regenerated from an auxin-autotrophic genotype of Zea mays (L.). Starting from immature embryos on hormone-free media, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium. In addition to fully-developed embryos, adventitious embryos are also formed under suitable culture conditions (6-9% of sucrose in the medium). When the sucrose content is reduced to 2-3% and 2,4-dichlorophenoxyacetic acid is added, soft, granular calli are formed which consist of embryogenic cell aggregates (type II callus). After subculturing the type II callus in the form of a cell suspension culture, totipotent protoplasts can be isolated. From these protoplasts, the maize plants according to the invention are regenerated.

    摘要翻译： 在短时间内可再生再生的正常肥沃植物的原生质体可以从玉米的生长素 - 自养基因型（L.）中再生。 从无激素培养基上的未成熟胚开始，在幼苗的基础上形成生长素 - 自养的胚性愈伤组织，所述愈伤组织在无激素培养基上传代培养的相当长的时间内保留其胚胎发生潜力。 除了完全发育的胚胎之外，在合适的培养条件（培养基中为6-9％的蔗糖）中也形成不定胚。 当蔗糖含量降低至2-3％，加入2,4-二氯苯氧乙酸时，形成由胚胎细胞聚集体（II型愈伤组织）组成的软质颗粒愈伤组织。 以细胞悬浮培养物形式传代培养II型愈伤组织后，可以分离全能的原生质体。 从这些原生质体中，再生根据本发明的玉米植物。