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公开(公告)号：US06221585B1
公开(公告)日：2001-04-24
申请号：US09007905
申请日：1998-01-15
IPC分类号： C12Q168
CPC分类号： C12Q1/6809 , C12Q1/6827 , C12Q2563/119 , C12Q2527/143 , C12Q2522/10
摘要： The present invention relates generally to the field of genomics. More particularly, the present invention relates to a method for gene identification beginning with user-selected input phenotypes. The method is referred to generally as the ValiGeneSMGene Identification method, or the VGIDSM method. The method employs nucleic acid mismatch binding protein chromatography to effect a molecular comparison of one phenotype with others. Genes are identified as having a specified function, or as causing or contributing to the cause or pathogenesis of a specified disease, or as associated with a specific phenotype, by virtue of their selection by the method. Identified genes may be used in development of reagents, drugs and/or combinations thereof useful in clinical or other settings for prognosis, diagnosis and/or treatment of diseases, disorders and/or conditions. The method is equally suited for gene identification for agricultural, bio-engineering, medical, veterinary, and many other applications.
摘要翻译： 本发明一般涉及基因组学领域。 更具体地说，本发明涉及从用户选择的输入表型开始的用于基因鉴定的方法。 该方法通常称为ValiGeneSMGene识别方法或VGIDSM方法。 该方法采用核酸错配结合蛋白色谱法对其中一种表型进行分子比较。 根据该方法的选择，将基因鉴定为具有指定功能，或引起或促成特定疾病的原因或发病机制，或与特定表型相关。 鉴定的基因可用于开发用于临床或其它设置的用于疾病，病症和/或病症的预后，诊断和/或治疗的试剂，药物和/或其组合。 该方法同样适用于农业，生物工程，医学，兽医等许多应用的基因鉴定。
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2.发明授权Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays 失效使用肽标记的寡核苷酸和抗体阵列检测核酸多态性的方法公开(公告)号：US06403309B1
公开(公告)日：2002-06-11
申请号：US09272970
申请日：1999-03-19
IPC分类号： C12Q168
CPC分类号： C12Q1/683 , C12Q1/6804 , C12Q1/6823 , C12Q1/6827 , C12Q1/6837 , C12Q2565/531 , C12Q2565/1025 , C12Q2525/107 , C12Q2521/30
摘要： The present invention is directed to methods and compositions for use in screening nucleic acid populations for nucleic acid polymorphisms. The methods, referred to generally as ValiGeneSM Mutation Screening, Peptide-Linked (VGMS-PL) methods, are specifically designed for high-throughput genotype mapping and gene expression analysis of animal and plant nucleic acids without requiring a PCR amplification step. In particular, the methods of the invention utilize oligonucleotide probes labeled with distinguishable and identifiable peptide tags, that are captured on addressable antibody arrays.
摘要翻译： 本发明涉及用于筛选核酸群体核酸多态性的方法和组合物。 通常称为ValiGeneSM突变筛选，肽连接（VGMS-PL）方法的方法专门用于动物和植物核酸的高通量基因型作图和基因表达分析，而不需要PCR扩增步骤。 特别地，本发明的方法利用在可寻址的抗体阵列上捕获的用可区分和可识别的肽标签标记的寡核苷酸探针。
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