公开(公告)号：US4870006A

公开(公告)日：1989-09-26

申请号：US926982

申请日：1986-11-04

申请人： Elizabeth A. Dragon ,  Stacey Sias

发明人： Elizabeth A. Dragon ,  Stacey Sias

IPC分类号： C07K14/44 ,  G01N33/569

CPC分类号： G01N33/56905 ,  C07K14/44 ,  Y10S435/81 ,  Y10S435/947 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/811 ,  Y10S530/806 ,  Y10S530/808 ,  Y10S530/822

摘要： T. Cruzi polypeptide antigens that react with serum from chagasic individuals and does not cross-react with serum from uninfected individuals or individuals infected with related parasites such as Leishmania is described. The DNA from T. Cruzi culture trypomastigotes and epimastigotes coding for antigenic material having a molecular weight of 70 kd is identified, sequenced, and inserted into a cloning vector, which, in turn, is inserted into a host cell line. The expressed polypeptide is immunologically reactive with sera from Chagas' disease infect patients. The cloned gene for the 70 kd polypeptide is expressed and purified and a diagnostic test for Chagas' disease comprising the synthesized polypeptide is described.

摘要翻译： 描述了与来自死亡个体的血清反应并且不与未感染的个体或感染有相关寄生虫如利什曼原虫的个体的血清交叉反应的T. Cruzi多肽抗原。 来自T. Cruzi培养物的分解重量为70kd的抗原性材料的trypomastigotes和epimastigotes的DNA被鉴定，测序并插入到克隆载体中，其又插入宿主细胞系中。 表达的多肽与来自恰加斯病感染患者的血清具有免疫反应性。 表达和纯化70kd多肽的克隆基因，并描述了包含合成多肽的查加斯病的诊断试验。