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1.发明授权High-cell density fed-batch fermentation process for producing recombinant protein 有权用于生产重组蛋白的高细胞密度补料分批发酵方法公开(公告)号：US08632995B2
公开(公告)日：2014-01-21
申请号：US11829703
申请日：2007-07-27
Applicant: Wei-Qiang Willie Sun , Earl Pursell
Inventor： Wei-Qiang Willie Sun , Earl Pursell
IPC: C12P21/06
Abstract: Methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and optionally continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions and compositions for use in the methods of the present invention, are provided.
Abstract translation: 用于生产蛋白质的方法，例如重组脑膜炎球菌2086蛋白质，使用补料分批发酵，在获得阈值参数后连续输入诱导物，以及任选连续输入碳源，例如恒定速率输入，以改善蛋白质 产生，以及用于本发明方法的高密度蛋白质组合物和组合物。
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2.发明申请Process for increasing rsv surface glycoprotein yields using a mutant strain of rsv 有权使用rsv的突变株提高rsv表面糖蛋白产量的方法公开(公告)号：US20060171955A1
公开(公告)日：2006-08-03
申请号：US10549533
申请日：2004-03-17
Applicant: Firelli Alonso-Caplen , Mark Cutler , Mirelli Fino , Earl Pursell , Robert Repetto
Inventor： Firelli Alonso-Caplen , Mark Cutler , Mirelli Fino , Earl Pursell , Robert Repetto
IPC: A61K39/12 , C12Q1/70 , C07K14/005
CPC classification number: C12N7/00 , A61K39/00 , A61K39/12 , A61K39/155 , A61K2039/55505 , C07K14/005 , C12N2760/18522 , C12N2760/18534 , C12N2760/18561 , G01N33/5091 , G01N33/56983
Abstract: A process for producing isolated and purified respiratory syncytial virus (RSV) fusion (F) and attachment (G) glycoproteins in eukaryotic cell cultures infected with RSV cold-passaged, temperature-sensitive mutant subgroup A2 strain cpts-248/404 results in at least a 5-fold increase in F and G protein yields when compared with the parent A2 strain. Immunogenic compositions comprising the F and/or G protein(s) produced by this process can be formulated for in vivo administration to a host to confer protection against disease caused by RSV.
Abstract translation: 在用RSV冷传代的温敏突变亚型A2菌株cpts-248/404感染的真核细胞培养物中产生分离和纯化的呼吸道合胞病毒（RSV）融合物（F）和附着物（G）糖蛋白的方法至少导致 与亲本A2株相比，F和G蛋白产量增加5倍。 包含通过该方法产生的F和/或G蛋白质的免疫原性组合物可以被配制用于体内施用于宿主以赋予针对由RSV引起的疾病的保护作用。
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3.发明授权Process for increasing RSV surface glycoprotein yields using a mutant strain of RSV 有权使用RSV突变菌株增加RSV表面糖蛋白产量的方法公开(公告)号：US07687240B2
公开(公告)日：2010-03-30
申请号：US10549533
申请日：2004-03-17
Applicant: Firelli Alonso-Caplen , Mark Cutler , Mirelli Fino , Earl Pursell , Robert Repetto
Inventor： Firelli Alonso-Caplen , Mark Cutler , Mirelli Fino , Earl Pursell , Robert Repetto
IPC: C12Q1/70 , G01N33/567 , C12N7/04
CPC classification number: C12N7/00 , A61K39/00 , A61K39/12 , A61K39/155 , A61K2039/55505 , C07K14/005 , C12N2760/18522 , C12N2760/18534 , C12N2760/18561 , G01N33/5091 , G01N33/56983
Abstract: A process for producing isolated and purified respiratory syncytial virus (RSV) fusion (F) and attachment (G) glycoproteins in eukaryotic cell cultures infected with RSV cold-passaged, temperature-sensitive mutant subgroup A2 strain cpts-248/404 results in at least a 5-fold increase in F and G protein yields when compared with the parent A2 strain. Immunogenic compositions comprising the F and/or G protein(s) produced by this process can be formulated for in vivo administration to a host to confer protection against disease caused by RSV.
Abstract translation: 在用RSV冷传代的温敏突变亚型A2菌株cpts-248/404感染的真核细胞培养物中产生分离和纯化的呼吸道合胞病毒（RSV）融合物（F）和附着物（G）糖蛋白的方法至少导致 与亲本A2株相比，F和G蛋白产量增加5倍。 包含通过该方法生产的F和/或G蛋白的免疫原性组合物可以配制成用于体内施用于宿主以赋予针对由RSV引起的疾病的保护作用。
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4.发明申请High-Cell Density Fed-Batch Fermentation Process for Producing Recombinant Protein 有权用于产生重组蛋白的高细胞密度补料分批发酵方法公开(公告)号：US20080026425A1
公开(公告)日：2008-01-31
申请号：US11829703
申请日：2007-07-27
Applicant: Wei-Qiang Willie Sun , Earl Pursell
Inventor： Wei-Qiang Willie Sun , Earl Pursell
Abstract: Methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and optionally continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions and compositions for use in the methods of the present invention, are provided.
Abstract translation: 用于生产蛋白质的方法，例如重组脑膜炎球菌2086蛋白质，使用补料分批发酵，在获得阈值参数后连续输入诱导物，以及任选连续输入碳源，例如恒定速率输入，以改善蛋白质 产生，以及用于本发明方法的高密度蛋白质组合物和组合物。
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