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公开(公告)号：US20190119687A1
公开(公告)日：2019-04-25
申请号：US16157692
申请日：2018-10-11
发明人： Ya-Shan Cheng , Tzu-Hui Wu , Cheng-Yen Lin , Hui-Lin Lai , Cheng-Bin Zheng , Ting-Yung Huang , I-Hsuan Lin , Jian-Wen Huang , Chun-Chi Chen , Rey-Ting Guo
CPC分类号： C12N15/52 , C12N9/2402 , C12N15/102 , C12Y302/01037 , C12Y302/01072 , C12Y302/01177
摘要： A xylosidase having improved enzymatic activity is disclosed. The amino acid sequence of the xylosidase is a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of phenylalanine at position 35 with glutamate, and/or a substitution of glutamine at position 41 with histidine.
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公开(公告)号：US09944915B2
公开(公告)日：2018-04-17
申请号：US15381438
申请日：2016-12-16
发明人： Rey-Ting Guo , Ya-Shan Cheng , Jian-Wen Huang , Tzu-Hui Wu , Hui-Lin Lai , Cheng-Yen Lin , Tsung-Yu Ko
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： A cellulase having improved thermostability is disclosed. The cellulase comprises a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is adding a cysteine in N terminal and adding a glycine and a cysteine or adding a proline and a cysteine in C terminal.
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公开(公告)号：US08865444B1
公开(公告)日：2014-10-21
申请号：US14019263
申请日：2013-09-05
发明人： Rey-Ting Guo , Jian-Wen Huang , Ya-Shan Cheng , Tzu-Hui Wu , Hui-Lin Lai , Cheng-Yen Lin , Ting-Yung Huang
IPC分类号： C12N9/24
CPC分类号： C12N9/2491 , C12Y302/01025
摘要： A β-mannanase having increased enzymaic activity is disclosed. The β-mannanase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Tyrosine at position 216 with Tryptophan.
摘要翻译： 公开了具有增加的酶活性的α-甘露聚糖酶。 β-甘露聚糖酶具有SEQ ID NO：2的修饰的氨基酸序列，其中修饰是用色氨酸取代位置216处的酪氨酸。
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公开(公告)号：US10563185B1
公开(公告)日：2020-02-18
申请号：US16363863
申请日：2019-03-25
发明人： Hui-Lin Lai , Ya-Shan Cheng , Tzu-Hui Wu , Cheng-Yen Lin , Ting-Yung Huang , I-Hsuan Lin , Cheng-Bin Zheng
IPC分类号： C12N9/24
摘要： A beta-glucosidase having improved enzymatic activity is disclosed. The amino acid sequence of the beta-glucosidase is a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of tyrosine at position 286 with phenylalanine, or a substitution of asparagine at position 639 with glutamate.
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公开(公告)号：US20150031110A1
公开(公告)日：2015-01-29
申请号：US14337884
申请日：2014-07-22
发明人： Rey-Ting Guo , Tzu-Hui Wu , Ya-Shan Cheng , Jian-Wen Huang , Hui-Lin Lai , Cheng-Yen Lin , Ting-Yung Huang
IPC分类号： C12N9/16
CPC分类号： C12Y301/03008 , C12N9/16 , C12Y301/03026
摘要： A phytase having improved enzymaic activity is disclosed. The phytase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Valine at position 90 with Threonine. Alternatively, the phytase has a modified amino acid sequence of SEQ ID NO: 6, wherein the modification is a substitution of Asparagine at position 204 with Alanine or a substitution of Serine at position 206 with Alanine.
摘要翻译： 公开了具有改善的酶活性的植酸酶。 植酸酶具有SEQ ID NO：2的修饰的氨基酸序列，其中修饰是用苏氨酸取代第90位的缬氨酸。 或者，植酸酶具有SEQ ID NO：6的修饰的氨基酸序列，其中所述修饰是用氨基酸替代位置204的天冬酰胺或用丙氨酸替代位置206处的丝氨酸。
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公开(公告)号：US08722382B1
公开(公告)日：2014-05-13
申请号：US14039017
申请日：2013-09-27
发明人： Rey-Ting Guo , Ya-Shan Cheng , Jian-Wen Huang , Tzu-Hui Wu , Hui-Lin Lai , Cheng-Yen Lin , Ting-Yung Huang
IPC分类号： C12N9/42
CPC分类号： C12N9/248
摘要： A xylanase having increased enzymaic activity is disclosed. The xylanase has a modified amino acid sequence of SEQ ID NO: 2, wherein Tryptophan at position 125 is substituted with Phenylalanine and Phenylalanine at position 163 is substituted with Tryptophan.
摘要翻译： 公开了具有增加的酶活性的木聚糖酶。 木聚糖酶具有SEQ ID NO：2的修饰的氨基酸序列，其中125位的色氨酸被苯丙氨酸取代，163位的苯丙氨酸被色氨酸取代。
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公开(公告)号：US10385346B2
公开(公告)日：2019-08-20
申请号：US16157692
申请日：2018-10-11
发明人： Ya-Shan Cheng , Tzu-Hui Wu , Cheng-Yen Lin , Hui-Lin Lai , Cheng-Bin Zheng , Ting-Yung Huang , I-Hsuan Lin , Jian-Wen Huang , Chun-Chi Chen , Rey-Ting Guo
摘要： A xylosidase having improved enzymatic activity is disclosed. The amino acid sequence of the xylosidase is a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of phenylalanine at position 35 with glutamate, and/or a substitution of glutamine at position 41 with histidine.
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公开(公告)号：US20170121699A1
公开(公告)日：2017-05-04
申请号：US15334827
申请日：2016-10-26
发明人： Rey-Ting Guo , Ya-Shan Cheng , Jian-Wen Huang , Tzu-Hui Wu , Hui-Lin Lai , Cheng-Yen Lin , Tsung-Yu Ko
IPC分类号： C12N9/88
CPC分类号： C12N9/88 , C12Y402/02002
摘要： A pectinase having improved thermostability is disclosed. The pectinase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of serine at position 181 with phenylalanine, methionine or leucine.
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公开(公告)号：US20140315273A1
公开(公告)日：2014-10-23
申请号：US14019263
申请日：2013-09-05
发明人： Rey-Ting Guo , Jian-Wen Huang , Ya-Shan Cheng , Tzu-Hui Wu , Hui-Lin Lai , Cheng-Yen Lin , Ting-Yung Huang
IPC分类号： C12N9/24
CPC分类号： C12N9/2491 , C12Y302/01025
摘要： A β-mannanase having increased enzymaic activity is disclosed. The β-mannanase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Tyrosine at position 216 with Tryptophan.
摘要翻译： 公开了具有增加的酶活性的α-甘露聚糖酶。 所述β-甘露聚糖酶具有SEQ ID NO：2的修饰的氨基酸序列，其中所述修饰是用色氨酸取代位置216处的酪氨酸。
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公开(公告)号：US10233430B1
公开(公告)日：2019-03-19
申请号：US16130859
申请日：2018-09-13
发明人： Ya-Shan Cheng , Tzu-Hui Wu , Cheng-Yen Lin , Hui-Lin Lai , Cheng-Bin Zheng , Ting-Yung Huang , I-Hsuan Lin , Jian-Wen Huang , Chun-Chi Chen , Rey-Ting Guo
摘要： A glucose oxidase having improved thermostability is disclosed. The amino acid sequence of the glucose oxidase is a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of glutamate at position 129 with proline, and/or a substitution of glutamine at position 243 with valine.
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