公开(公告)号：US20100022390A1

公开(公告)日：2010-01-28

申请号：US12520758

申请日：2007-12-26

申请人： Daniel J. Cosgrove

发明人： Daniel J. Cosgrove

IPC分类号： A01N63/00 ,  A01H5/00 ,  A01N57/00

CPC分类号： C12N15/8286 ,  A01N63/02 ,  A01N63/04 ,  Y02A40/162

摘要： A method for managing resistance in a plot of pest resistant crop plants is provided herein. The method includes cultivating a first pest resistant crop plant in a plot in one planting cycle, and successively cultivating in a second planting cycle a second pest resistant crop plant in the same plot, wherein the first and the second pest resistant crop plants are pesticidal to corn rootworm but through a different mode of pesticidal action.

摘要翻译： 本文提供了一种防治害虫作物的抗性的方法。 该方法包括在一个种植周期的情况下培育第一害虫抗性作物植物，并且在相同情况下在第二种植周期中连续培养第二害虫抗性作物植物，其中所述第一和第二害虫抗性作物植物是杀虫剂 玉米根虫通过不同的杀虫作用方式。

公开(公告)号：US20080226753A1

公开(公告)日：2008-09-18

申请号：US10599307

申请日：2005-03-29

申请人： Daniel J. Cosgrove

发明人： Daniel J. Cosgrove

IPC分类号： A01N65/00 ,  A01N37/08 ,  A01N43/40 ,  A01N33/26 ,  A01N43/647 ,  A01N57/12 ,  A01N43/36 ,  A01N43/56 ,  A01N37/44 ,  A61K38/16 ,  A01N43/04 ,  A01P7/00

CPC分类号： C12N15/8286 ,  A01N25/00 ,  Y02A40/162

摘要： The present invention discloses Resistance Management (RM) practices that are critical to safeguarding Bacillus thuringiensis as a natural resource and to sustaining genetically modified crops that express Bt toxins for managing ECB and WCRW. The methods involve blending seed transformed with a nucleic acid encoding a different pesticidal protein, where both proteins target the same pest, but use different modes of pesticidal action. The seed can be also treated with pesticidal agents.

摘要翻译： 本发明公开了对保护苏云金芽孢杆菌作为天然资源至关重要的抗性管理（RM）实践以及维持表达Bt毒素用于管理ECB和WCRW的转基因作物。 所述方法包括将转化的编码不同杀虫蛋白的核酸的混合物混合，其中两种蛋白质靶向相同的害虫，但使用不同的杀虫作用模式。 种子也可以用杀虫剂处理。

公开(公告)号：US06255466B1

公开(公告)日：2001-07-03

申请号：US09092160

申请日：1998-06-05

申请人： Daniel J. Cosgrove ,  Simon McQueen-Mason ,  Mark Guiltinan ,  Tatyana Shcherban ,  Jun Shi

发明人： Daniel J. Cosgrove ,  Simon McQueen-Mason ,  Mark Guiltinan ,  Tatyana Shcherban ,  Jun Shi

IPC分类号： C07H2102

CPC分类号： C12N9/00 ,  C07K14/415 ,  C12N9/2437 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8261 ,  D21H17/22 ,  Y02A40/146

摘要： The sequence is disclosed both as the nucleic acid sequence SEQ ID NO: 1 and as the corresponding amino acid sequence SEQ ID NO: 7. Five other amino acid sequences are also disclosed, two from rice and three from Arabidopsis, SEQ ID NO: 2 through SEQ ID NO:6, respectively.

摘要翻译： 该序列同时被公开为核酸序列SEQ ID NO：1，并作为相应的氨基酸序列SEQ ID NO：7。另外还公开了另外5个氨基酸序列，两个来自水稻，三个来自拟南芥，SEQ ID NO：2 通过SEQ ID NO：6。

公开(公告)号：US5990283A

公开(公告)日：1999-11-23

申请号：US834327

申请日：1997-04-15

申请人： Daniel J. Cosgrove ,  Simon McQueen-Mason

发明人： Daniel J. Cosgrove ,  Simon McQueen-Mason

IPC分类号： C07K14/415 ,  C12N9/42 ,  C12N15/82 ,  C07K15/00

CPC分类号： C12N9/2437 ,  C07K14/415 ,  C12N15/8261

摘要： Plant cell expansion is regulated by wall relaxation and yielding, which is thought to be catalyzed by elusive "wall loosening" enzymes. By employing a reconstitution approach, we initially found that a crude protein extract from the cell walls of growing cucumber seedlings possessed the ability to induce the extension of isolated cell walls. This activity was restricted to the growing region of the stem and could induce the extension of isolated cell walls from various dicots and monocots, but was less effective on grass coleoptile walls. Sequential HPLC fractionation of the active wall extract revealed two proteins with molecular masses of 29 and 30 kD, as measured by SDS-PAGE, associated with such activity. Each protein, by itself, could induce wall extension without detectable hydrolytic breakdown of the wall We proposed the name "expansins" for this class of proteins. Expansins have been isolated from various plant sources including oat, cucumber, broccoli, celery, tomato, cotton, cabbage, and corn, and also from snail and its feces. These proteins weaken the intermolecular bonds between plant wall polysaccharides. They decrease the mechanical strength of commercial products made from polysaccharides, such as paper, and therefore present a novel approach in developing new technologies in industries which make use of such polysaccharides, such as in the paper industry, in the applications of polysaccharide gums and related products. These proteins moreover present a novel approach in the control of plant growth.

摘要翻译： 植物细胞膨胀受壁松弛和产量的调节，这被认为是由难以捉摸的“壁松动”酶催化的。 通过采用重组方法，我们最初发现，生长黄瓜幼苗细胞壁的粗蛋白提取物具有诱导分离的细胞壁延伸的能力。 该活动仅限于茎的生长区域，并且可以诱导来自各种双子叶植物和单子叶植物的分离的细胞壁的延伸，但是对于草胚芽鞘壁效果较差。 活性壁提取物的顺序HPLC分级分离显示分子量为29和30kD的蛋白质，通过SDS-PAGE测量，与这种活性相关。 每种蛋白质本身可以诱导壁延伸，而不会有可检测到的壁的水解破坏。我们提出了这类蛋白质的名称“膨胀物”。 膨胀体已经从各种植物来源分离，包括燕麦，黄瓜，西兰花，芹菜，番茄，棉花，卷心菜和玉米，以及蜗牛及其粪便。 这些蛋白质削弱植物壁多糖之间的分子间键。 它们降低了由多糖制成的商业产品的机械强度，如纸张，因此在开发利用这种多糖的行业中开发新技术的新方法，如造纸工业，多糖胶和相关的应用 产品。 这些蛋白质还提供了一种控制植物生长的新方法。

公开(公告)号：US20080201123A1

公开(公告)日：2008-08-21

申请号：US11893509

申请日：2007-08-16

申请人： Daniel J. Cosgrove

发明人： Daniel J. Cosgrove

IPC分类号： G06G7/48 ,  C07K14/42 ,  C12Q1/25

CPC分类号： C07K14/415

摘要： The invention relates to crystal structure and activities of Beta-expansins and grass pollen allergens and identification of key regions essential to maximize activity and to identify sequence motifs which correlate with activity.

摘要翻译： 本发明涉及β-延展蛋白和草花粉过敏原的晶体结构和活性，以及​​确定最大限度活性和识别与活性相关的序列基序所必需的关键区域。

公开(公告)号：US06682738B1

公开(公告)日：2004-01-27

申请号：US09071252

申请日：1998-05-01

申请人： Daniel J. Cosgrove

发明人： Daniel J. Cosgrove

IPC分类号： A61K3578

CPC分类号： C12N15/8261 ,  A61K38/168 ,  C07K14/415 ,  Y02A40/146

摘要： The present invention relates to proteins belonging to a novel class of proteins designated as &bgr;-expansins, a composition comprising such proteins, isolated polynucleotides encoding &bgr;-expansins, methods for using the polynucleotides and proteins of the invention and methods for identifying, isolating and purifying expansins, including &agr; and &bgr;-expansins. Beta-expansins of the invention have the property of altering physical properties of a plant cell wall, such as for example by loosening or expanding plant cell walls.

摘要翻译： 本发明涉及属于称为β-延蛋白的新型蛋白质的蛋白质，包含这种蛋白质的组合物，编码β-延伸蛋白质的分离的多核苷酸，使用本发明的多核苷酸和蛋白质的方法以及用于鉴定，分离和纯化的方法 膨胀物，包括α和β扩张体。 本发明的β-膨胀物具有改变植物细胞壁的物理性质的性质，例如通过松动或扩大植物细胞壁。

公开(公告)号：US20100184224A1

公开(公告)日：2010-07-22

申请号：US12177992

申请日：2008-07-23

申请人： LIAN-CHAO LI ,  DANIEL J. COSGROVE

发明人： LIAN-CHAO LI ,  DANIEL J. COSGROVE

IPC分类号： C12N5/04 ,  C08B1/00

CPC分类号： C07K14/415 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention provides nucleic acids and polypeptide sequences for a novel class of expansin-related proteins, designated group 2/3 allergen, which comprise the group 2 and group 3 allergens from grass, a purified group 3 allergen Lol p 3, and method of using the nucleic acids sequences and proteins of the invention. Group 2/3 allergens of the invention are significant wall-loosening agents. They are capable of altering cell wall properties, which may effect growth, flexibility, and mechanical strength in tissues in which they are expressed.

摘要翻译： 本发明提供了一种新型扩展蛋白相关蛋白的核酸和多肽序列，其命名为组2/3变应原，其包含来自草的组2和组3变应原，纯化组3变应原Lol p 3，以及方法 使用本发明的核酸序列和蛋白质。 本发明的组2/3变应原是显着的壁松动剂。 它们能够改变细胞壁性质，这可以影响其表达的组织中的生长，柔性和机械强度。

公开(公告)号：US20100029725A1

公开(公告)日：2010-02-04

申请号：US12520750

申请日：2007-12-26

申请人： Daniel J. Cosgrove ,  Paula M. Davis ,  Robert C. Iwig

发明人： Daniel J. Cosgrove ,  Paula M. Davis ,  Robert C. Iwig

IPC分类号： A01N43/647 ,  A01M1/20 ,  A01C1/00 ,  A01N43/56 ,  A01N43/36 ,  A01N53/10 ,  A01N37/52 ,  A01G7/00 ,  A01P13/00 ,  A01P7/04 ,  A01P3/00 ,  A01P5/00 ,  A01P1/00

CPC分类号： C12N15/8286 ,  A01N63/02 ,  A01N63/04 ,  Y02A40/162

摘要： Insect refuge strategies are described for the management of insect resistance development. The present invention relates generally to the control of pests that cause damage to crop plants, and in particular to corn plants, by their feeding activities directed to root damage, and more particularly to the control of such plant pests by exposing target pests to seeds or mixtures of seeds having multiple different modes of action. The first one or more transgenes and the second one or more transgenes are each, respectively, insecticidal to the same target insect but have different modes of action, and bind either semi-competitively or non-competitively to different binding sites in the target pest. In addition, the treatment of such seed with a chemical or peptide-associated pesticide prior to planting the seed is also disclosed.

摘要翻译： 昆虫避难策略被描述为管理昆虫抗性发展。 本发明一般涉及通过其针对根损伤的饲养活动，特别是通过将目标害虫暴露于种子或其中来控制这种植物害虫，从而对作物，特别是玉米植物造成损害的害虫的控制， 具有多种不同作用方式的种子混合物。 第一个或多个转基因和第二个一个或多个转基因各自分别对相同的目标昆虫具有杀虫作用，但具有不同的作用模式，并且与竞争性或非竞争性地结合到目标害虫中的不同结合位点。 此外，还公开了在种植种子之前用化学或肽相关农药处理这种种子。

公开(公告)号：US07226756B2

公开(公告)日：2007-06-05

申请号：US10670009

申请日：2003-09-24

申请人： Daniel J. Cosgrove ,  Simon McQueen-Mason ,  Mark J. Guiltinan ,  Tatyana Shcherban ,  Jun Shi

发明人： Daniel J. Cosgrove ,  Simon McQueen-Mason ,  Mark J. Guiltinan ,  Tatyana Shcherban ,  Jun Shi

IPC分类号： C07K14/415 ,  C12N15/82 ,  C12N9/42 ,  C12N9/00

CPC分类号： C07K14/415 ,  C12N9/00 ,  C12N15/8242 ,  C12N15/8261 ,  D21C5/027 ,  D21H17/22 ,  Y02A40/146 ,  Y02W30/648

摘要： This invention relates to a new class of proteins called expansins, and methods related thereto are presented. This class of proteins can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose. Two proteins have been isolated from washed wall fragments of cucumber hypocotyls, referred to as “cucumber expansin-29” and “cucumber expansin-30”. Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified in Arabidopsis and an additional two in rice.

摘要翻译： 本发明涉及一类称为膨胀素的新型蛋白质，并提出了与之相关的方法。 这类蛋白质可以表征为植物细胞壁延伸的催化剂和纯纤维素中氢键的弱化。 已经从黄瓜下胚轴的洗涤的壁碎片中分离出两种蛋白质，称为“黄瓜扩展蛋白-29”和“黄瓜扩展蛋白30”。 此外，对来自纯化的cEx-29蛋白的三个肽片段进行测序，然后设计寡核苷酸引物以使用来自黄瓜幼苗的cDNA模板进行聚合酶链反应扩增一部分扩展蛋白cDNA，然后使用PCR片段筛选 用于鉴定全长克隆的cDNA文库。 另外一种扩增蛋白已经从燕麦胚芽鞘（oat expansin oEx-29）中分离出来，而在拟南芥中已经鉴定了三种扩增蛋白序列，另外两种在水稻中被鉴定出来。

公开(公告)号：US5959082A

公开(公告)日：1999-09-28

申请号：US440517

申请日：1995-05-12

申请人： Daniel J. Cosgrove ,  Simon McQueen-Mason ,  Mark Guiltinan ,  Tatyana Shcherban ,  Jun Shi

发明人： Daniel J. Cosgrove ,  Simon McQueen-Mason ,  Mark Guiltinan ,  Tatyana Shcherban ,  Jun Shi

IPC分类号： C07K14/415 ,  C12N9/00 ,  C12N9/42 ,  C12N15/82 ,  D21H17/22 ,  A61K35/78 ,  A23J1/14 ,  C07K17/00

CPC分类号： C12N9/00 ,  C07K14/415 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8261 ,  C12N9/2437 ,  D21H17/22

摘要： A new class of proteins and methods related thereto are presented. The proteins, which can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose, are referred to as expansins. Two proteins have been isolated by fractionation techniques from washed wall fragments of cucumber hypocotyls, referred to as "cucumber expansin-29" and "cucumber expansin-30" (abbreviated cEx-29 and cEx-30, with respect to their apparent relative masses as determined by SDS-PAGE). Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified in Arabidopsis and an additional two in rice. Expansins appear to be broadly distributed throughout the plant kingdom and can be identified in stem and leaf vegetables (i.e., broccoli, cabbage), fruit and seed vegetables (i.e., tomato), fiber crops and cereals (i.e., corn), and forest and ornamental crops (i.e., cotton). An expansin, generally, is a protein which has at least about 60% sequence similarity with the amino acid sequence shown in SEQ. ID. NO:1, and preferably has at least about 70% sequence similarity with SEQ. ID. NO:1.

摘要翻译： 提出了一类新的相关蛋白质和方法。 可以表征为植物细胞壁延伸的催化剂和纯纤维素中氢键的弱化的蛋白质被称为膨胀物。 通过分离技术从黄瓜下胚轴的洗涤壁片段（称为“黄瓜扩展蛋白-29”和“黄瓜扩展子30”）（缩写为cEx-29和cEx-30），通过分离技术分离了两种蛋白质，其相对于其表观相对质量为 通过SDS-PAGE测定）。 此外，对来自纯化的cEx-29蛋白的三个肽片段进行测序，然后设计寡核苷酸引物以使用来自黄瓜幼苗的cDNA模板进行聚合酶链反应扩增一部分扩展蛋白cDNA，然后使用PCR片段筛选 一个cDNA文库，用于鉴定全长克隆。 另外一种扩增蛋白已经从燕麦胚芽鞘（oat expansin oEx-29）中分离出来，而在拟南芥中已经鉴定了三种扩增蛋白序列，另外两种在水稻中被鉴定出来。 扩展体似乎广泛分布在整个植物界，可以在茎叶蔬菜（即西兰花，卷心菜），水果和种子蔬菜（即番茄），纤维作物和谷类（即玉米），以及森林和 观赏作物（即棉花）。 通常，扩展蛋白是与SEQ ID NO：1所示氨基酸序列具有至少约60％序列相似性的蛋白质。 ID。 NO：1，优选与SEQ ID NO：1至少约70％的序列相似性。 ID。 NO：1。