公开(公告)号：US08609359B2

公开(公告)日：2013-12-17

申请号：US13318054

申请日：2010-04-23

Applicant: Lara Hasan ,  Michel-Angelo Sciotti ,  Thomas Jermann ,  Jakob Matthias Weber ,  Daniel Gygax ,  André Scholer

Inventor： Lara Hasan ,  Michel-Angelo Sciotti ,  Thomas Jermann ,  Jakob Matthias Weber ,  Daniel Gygax ,  André Scholer

IPC: C12Q1/26

CPC classification number: C12Q1/26 ,  C12Q1/32

Abstract: Methods to determine the concentration of gamma-hydroxy butyric acid (GHB) in a sample as well as compositions and a kit suitable for carrying out said methods. Also, the use of the methods for application on a microtiter plate or an auto-analyzer.

Abstract translation: 确定样品中γ-羟基丁酸（GHB）浓度的方法以及适于实施所述方法的组合物和试剂盒。 此外，使用在微量滴定板或自动分析仪上应用的方法。

公开(公告)号：US5098841A

公开(公告)日：1992-03-24

申请号：US360802

申请日：1989-06-02

Applicant: Oreste Ghisalba ,  Daniel Gygax ,  Rente Lattmann ,  Hans-Peter Schar ,  Elke Schmidt ,  Gottfried Sedelmeier

Inventor： Oreste Ghisalba ,  Daniel Gygax ,  Rente Lattmann ,  Hans-Peter Schar ,  Elke Schmidt ,  Gottfried Sedelmeier

IPC: C12P7/52 ,  C07C51/367 ,  C07K5/078 ,  C12P7/42 ,  C12R1/45

CPC classification number: C12P7/42 ,  C07C51/367 ,  C07K5/06139

Abstract: The invention relates to a preferably continuous process for the preparation of R- or S-2-hydroxy-4-phenylbutyric acid in very high enantiomeric purity, which comprises reducing 2-oxo-4-phenylbutyric acid with the enzyme D-lactate dehydrogenase from Staphylococcus epidermidis or with the enzyme L-lactate dehydrogenase from bovine heart, respectively, in the presence of NAD(H) and formate or ethanol and formate dehydrogenase or alcohol dehydrogenase, respectively. R-2-hydroxy-4-phenylbutyric acid is a valuable intermediate in the preparation of ACE inhibitors or their precursors.

Abstract translation: 本发明涉及用于制备非常高对映体纯度的R-或S-2-羟基-4-苯基丁酸的优选连续方法，其包括用D-乳酸脱氢酶酶将2-氧代-4-苯基丁酸与 分别在NAD（H）和甲酸盐或乙醇和甲酸脱氢酶或醇脱氢酶存在下分别从牛心脏分泌表皮葡萄球菌或L-乳酸脱氢酶酶。 R-2-羟基-4-苯基丁酸是制备ACE抑制剂或其前体的有价值的中间体。

公开(公告)号：US20120052520A1

公开(公告)日：2012-03-01

申请号：US13318054

申请日：2010-04-23

Applicant: Lara Hasan ,  Michel-Angelo Sciotti ,  Thomas Jermann ,  Jakob Matthias Weber ,  Daniel Gygax ,  André Scholer

Inventor： Lara Hasan ,  Michel-Angelo Sciotti ,  Thomas Jermann ,  Jakob Matthias Weber ,  Daniel Gygax ,  André Scholer

IPC: C12Q1/32 ,  C12Q1/26

CPC classification number: C12Q1/26 ,  C12Q1/32

Abstract: Methods to determine the concentration of gamma-hydroxy butyric acid (GHB) in a sample as well as compositions and a kit suitable for carrying out said methods. Also, the use of the methods for application on a microtiter plate or an auto-analyzer.

Abstract translation: 确定样品中γ-羟基丁酸（GHB）浓度的方法以及适于实施所述方法的组合物和试剂盒。 此外，使用在微量滴定板或自动分析仪上应用的方法。

公开(公告)号：US09574221B2

公开(公告)日：2017-02-21

申请号：US14124958

申请日：2012-06-11

Applicant: Jakob Weber ,  Lara Hasan ,  Thomas Jermann ,  Michel-Angelo Sciotti ,  Daniel Gygax ,  Andre Scholer

Inventor： Jakob Weber ,  Lara Hasan ,  Thomas Jermann ,  Michel-Angelo Sciotti ,  Daniel Gygax ,  Andre Scholer

IPC: C12Q1/48 ,  C12Q1/28 ,  G01N33/82 ,  C12Q1/527 ,  C12Q1/26

CPC classification number: C12Q1/48 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/527 ,  G01N33/82 ,  G01N2333/90238 ,  G01N2333/988

Abstract: The invention relates to methods for the determination of vitamin B6 in samples as well as to reagent compositions for assaying a sample for vitamin B6 and to a test kit suitable for carrying out the methods according to the present invention. Further, the invention relates to the use of such methods for the application to different analyzing devices such as micro-titer plate readers and fully automated clinical chemistry analyzers (autoanalyzers).

Abstract translation: 本发明涉及用于测定样品中维生素B6的方法以及用于测定维生素B6样品的试剂组合物以及适用于实施本发明方法的测试试剂盒。 此外，本发明涉及这种方法用于不同分析装置的应用，例如微量滴定板读数器和全自动临床化学分析仪（自动分析仪）。

公开(公告)号：US20140308691A1

公开(公告)日：2014-10-16

申请号：US14124958

申请日：2012-06-11

Applicant: Jakob Weber ,  Lara Hasan ,  Thomas Jermann ,  Michel-Angelo Sciotti ,  Daniel Gygax ,  Andre Scholer

Inventor： Jakob Weber ,  Lara Hasan ,  Thomas Jermann ,  Michel-Angelo Sciotti ,  Daniel Gygax ,  Andre Scholer

IPC: C12Q1/48 ,  C12Q1/26 ,  C12Q1/28

CPC classification number: C12Q1/48 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/527 ,  G01N33/82 ,  G01N2333/90238 ,  G01N2333/988

Abstract: The invention relates to methods for the determination of vitamin B6 in samples as well as to reagent compositions for assaying a sample for vitamin B6 and to a test kit suitable for carrying out the methods according to the present invention. Further, the invention relates to the use of such methods for the application to different analyzing devices such as micro-titer plate readers and fully automated clinical chemistry analyzers (autoanalyzers).

Abstract translation: 本发明涉及用于测定样品中维生素B6的方法以及用于测定维生素B6样品的试剂组合物以及适用于实施本发明方法的测试试剂盒。 此外，本发明涉及这种方法用于不同分析装置的应用，例如微量滴定板读数器和全自动临床化学分析仪（自动分析仪）。

公开(公告)号：US5071750A

公开(公告)日：1991-12-10

申请号：US606944

申请日：1990-10-31

Applicant: Udo Kragl ,  Christian Wandrey ,  Oreste Ghisalba ,  Daniel Gygax

Inventor： Udo Kragl ,  Christian Wandrey ,  Oreste Ghisalba ,  Daniel Gygax

IPC: C12P19/26

CPC classification number: C12P19/26

Abstract: A process for obtaining N-acetylneuraminic acid from N-acetylglucosamine is disclosed. The process is carried out in a reactor which contains both N-acylglucosamine-2-epimerase (E.C. 5.1.3.8) which isomerizes GlcNAc into ManNAc, and N-acetylneuraminic acid pyruvate lyase (E.C. 4.1.3.3) which catalyzes the reaction of the resulting ManNAc with pyruvic acid to give Neu5Ac. GlcNAc and Pyr are fed into the reactor and Neu5Ac is obtained in the outflow. The process is preferably carried out continuously and in particular in an enzyme membrane reactor at pH 7.5 and 25.degree. C., especially using residence times of 0.2 to 10 h, and with an excess of GlcNAc in comparison with Pyr which is subsequently added if necessary. Epimerase and lyase are preferably present in the reactor in a ratio of activities which is equivalent to the reciprocal value of the quotient of the conversion rates.

Abstract translation: 公开了从N-乙酰葡糖胺获得N-乙酰神经氨酸的方法。 该方法在含有将GlcNac异构化成ManNac的N-酰基葡糖胺-2-差向异构酶（EC 5.1.3.8）和N-乙酰神经氨酸丙酮酸裂合酶（EC 4.1.3.3）的反应器中进行，其催化所得到的 ManNac与丙酮酸反应得到Neu5Ac。 将GlcNAc和Pyr进料到反应器中，并在流出物中获得Neu5Ac。 该方法优选连续地，特别是在pH 7.5和25℃的酶膜反应器中进行，特别是使用0.2至10小时的停留时间，以及与Pyr相比过量的GlcNac，如果需要，随后加入 。 差向异构酶和裂解酶优选以相当于转化率的商的倒数值的活性比例存在于反应器中。