公开(公告)号：US5419079A

公开(公告)日：1995-05-30

申请号：US177573

申请日：1994-01-05

申请人： Bingjun Wang ,  Luye Wang ,  Xiaohui Liu

发明人： Bingjun Wang ,  Luye Wang ,  Xiaohui Liu

IPC分类号： A01H4/00 ,  A01B79/00 ,  C12N5/00

CPC分类号： A01H4/005 ,  Y10S47/03 ,  Y10S47/06

摘要： This invention discloses a method of producing virus-free potato minitubers, which includes the steps of selecting virus-free test-tube plantlets and stem sections cut from seedlings grown 30 days after cuttage of such virus-free test-tube plantlets as cuttaging seedlings, choosing vermiculite as cuttage medium, treating the cuttaging seedlings with plant growth regulators, keeping the growth conditions of the cuttaging seedlings at 25.degree. C. during the daytime and 12.degree.-17.degree. C. at night, relative humidity 95-100% during the early stage and 60-70% at the later stage, and a light duration of ten hours a day with illuminance of 400-600 lux, along with suitable field management of water and fertilizer. The virus-free potato minitubers can be harvested in 30 to 60 days.

摘要翻译： 本发明公开了一种生产无病毒马铃薯小型微生物的方法，其包括如下步骤：从切除这种无病毒试管苗后30天生长的切片幼苗中选出无病毒试管苗和茎切片， 选择蛭石作为剪切培养基，用植物生长调节剂处理切割幼苗，在白天和12℃-17℃，白昼期间保持切割幼苗的生长条件保持在25℃，相对湿度95-100％ 早期阶段为60-70％，光照持续时间为每天10小时，照度为400-600勒克斯，适用于水肥的现场管理。 无病毒的马铃薯小提取物可以在30至60天内收获。

公开(公告)号：US20100168386A1

公开(公告)日：2010-07-01

申请号：US12442527

申请日：2007-09-04

申请人： Aimin Ji ,  Ou Che ,  Leilei Ma ,  Dan Su ,  Hanzhang Ma ,  Xiaodong Li ,  Liang Sun ,  Bingjun Wang ,  Zhongyi Zhang

发明人： Aimin Ji ,  Ou Che ,  Leilei Ma ,  Dan Su ,  Hanzhang Ma ,  Xiaodong Li ,  Liang Sun ,  Bingjun Wang ,  Zhongyi Zhang

IPC分类号： C07K14/475 ,  C07H21/04 ,  C12N15/63 ,  C12N5/00

CPC分类号： C07K14/475 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/01

摘要： Provided are fusion proteins which comprise a first region having at least 75% homology with the amino acid sequence of neurotrophin, and a second region, located at the C-terminal of the first region, having at least 75% homology with the amino acid sequence of protein transduction domain (PTD). Also provided is encoding gene and usage thereof. The fusion protein can translocate through cell membrane or even the blood-brain barrier (BBB). The fusion protein can be used to treat various central nervous system degenerative diseases and peripheral neuropathy.

摘要翻译： 提供了融合蛋白，其包含与神经营养蛋白的氨基酸序列具有至少75％同源性的第一区域和位于第一区域的C-末端的与氨基酸序列具有至少75％同源性的第二区域 的蛋白转导结构域（PTD）。 还提供了编码基因及其用途。 融合蛋白可以通过细胞膜或甚至血脑屏障（BBB）移位。 融合蛋白可用于治疗各种中枢神经系统退行性疾病和周围神经病变。