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    Methods of using analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamate 89, aspartate 101 or leucine 137
    1.
    发明授权
    Methods of using analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamate 89, aspartate 101 or leucine 137 有权
    使用在一个或多个位置谷氨酸盐89，天冬氨酸101或亮氨酸137突变的人碱性成纤维细胞生长因子类似物的方法

    公开(公告)号：US06737404B2

    公开(公告)日：2004-05-18

    申请号：US09826210

    申请日：2001-04-05

    申请人： Barry A. Springer ,  Michael W. Pantoliano ,  Celia M. Sharp

    发明人： Barry A. Springer ,  Michael W. Pantoliano ,  Celia M. Sharp

    IPC分类号： A61K3818

    CPC分类号： C07K14/503 ,  A61K38/00

    摘要： The present invention relates to novel muteins of human basic fibroblast growth factor with superagonist properties. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for stimulating cell division, treating a wound, treating ischemia, treating heart disease, treating neural injury, treating peripheral vascular disease, treating a gastric ulcer and treating a duodenal ulcer.

    摘要翻译： 本发明涉及具有超强调性质的人碱性成纤维细胞生长因子的新颖突变蛋白。 公开了蛋白质和各自编码的核酸物质。 本发明还体现了用于扩增所述核酸序列和产生所述突变蛋白的载体和宿主细胞。 还公开了刺激细胞分裂，治疗伤口，治疗缺血，治疗心脏病，治疗神经损伤，治疗外周血管疾病，治疗胃溃疡和治疗十二指肠溃疡的方法。

    Method for identifying conditions that facilitate recombinant protein folding
    2.
    发明授权
    Method for identifying conditions that facilitate recombinant protein folding 有权
    用于鉴定促进重组蛋白质折叠的条件的方法

    公开(公告)号：US06291192B1

    公开(公告)日：2001-09-18

    申请号：US09477724

    申请日：2000-01-05

    申请人： Michael W. Pantoliano ,  Eugenio C. Petrella ,  Francis R. Salemme ,  Barry A. Springer

    发明人： Michael W. Pantoliano ,  Eugenio C. Petrella ,  Francis R. Salemme ,  Barry A. Springer

    IPC分类号： G01N3353

    CPC分类号： C40B30/04 ,  B01L7/00 ,  B01L7/52 ,  C07K1/047 ,  C12Q1/6811 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/86 ,  G01N35/028 ,  G01N2500/00

    摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.

    摘要翻译： 本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。 该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到 从每个容器中的加热，产生作为每个容器的温度的函数的靶分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与 在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

    Microplate thermal shift assay for ligand development and multi-variable protein chemistry optimization
    3.
    发明授权
    Microplate thermal shift assay for ligand development and multi-variable protein chemistry optimization 有权
    用于配体开发和多变量蛋白质化学优化的微板热转移测定

    公开(公告)号：US06291191B1

    公开(公告)日：2001-09-18

    申请号：US09452203

    申请日：1999-12-02

    申请人： Michael W. Pantoliano ,  Eugenio C. Petrella ,  Alexander W. Rhind ,  Francis R. Salemme ,  Barry A. Springer

    发明人： Michael W. Pantoliano ,  Eugenio C. Petrella ,  Alexander W. Rhind ,  Francis R. Salemme ,  Barry A. Springer

    IPC分类号： G01N3353

    CPC分类号： C40B30/04 ,  B01L7/00 ,  B01L7/52 ,  C07K1/047 ,  C12Q1/6811 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/86 ,  G01N35/028 ,  G01N2500/00

    摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.

    摘要翻译： 本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。 该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到 从每个容器中的加热，产生作为每个容器的温度的函数的靶分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与 在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

    Method for determining conditions that facilitate protein crystallization
    6.
    发明授权
    Method for determining conditions that facilitate protein crystallization 有权
    确定促进蛋白质结晶的条件的方法

    公开(公告)号：US06268158B1

    公开(公告)日：2001-07-31

    申请号：US09458663

    申请日：1999-12-10

    申请人： Michael W. Pantoliano ,  Eugenio C. Petrella ,  Francis R. Salemme ,  Barry A. Springer

    发明人： Michael W. Pantoliano ,  Eugenio C. Petrella ,  Francis R. Salemme ,  Barry A. Springer

    IPC分类号： G01N3353

    CPC分类号： C40B30/04 ,  B01L7/00 ,  B01L7/52 ,  C07K1/047 ,  C12Q1/6811 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/86 ,  G01N35/028 ,  G01N2500/00

    摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.

    摘要翻译： 本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。 该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到 从每个容器中的加热，产生作为每个容器的温度的函数的目标分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与 在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

    Method for determining conditions that stabilize proteins
    7.
    发明授权
    Method for determining conditions that stabilize proteins 有权
    确定蛋白质稳定条件的方法

    公开(公告)号：US06232085B1

    公开(公告)日：2001-05-15

    申请号：US09458691

    申请日：1999-12-10

    申请人： Michael W Pantoliano ,  Eugenio C Petrella ,  Francis R Salemme ,  Barry A Springer

    发明人： Michael W Pantoliano ,  Eugenio C Petrella ,  Francis R Salemme ,  Barry A Springer

    IPC分类号： G01N3353

    CPC分类号： C40B30/04 ,  B01L7/00 ,  B01L7/52 ,  C07K1/047 ,  C12Q1/6811 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/86 ,  G01N35/028 ,  G01N2500/00

    摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.

    摘要翻译： 本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。 该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到 从每个容器中的加热，产生作为每个容器的温度的函数的目标分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与 在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

    Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes
    8.
    发明授权
    Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes 有权
    使用5-（4'-二甲基氨基苯基）-2-（4'-苯基）恶​​唑衍生物荧光染料的配体发育的微板热转移测定

    公开(公告)号：US06569631B1

    公开(公告)日：2003-05-27

    申请号：US09438357

    申请日：1999-11-12

    申请人： Michael W. Pantoliano ,  Eugenio C. Petrella ,  F. Raymond Salemme ,  Barry A. Springer

    发明人： Michael W. Pantoliano ,  Eugenio C. Petrella ,  F. Raymond Salemme ,  Barry A. Springer

    IPC分类号： G01N3353

    CPC分类号： G01N33/582 ,  G01N33/5375

    摘要： The present invention provides a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of unfolding due to a thermal change. The method comprises (a) contacting the target molecule with one molecule of a multiplicity of different molecules, in the presence of a 5-(4″-dimethylaminopheny-2-(4′-phenyl)oxazole derivative dye, in each of a multiplicity of containers; (b) simultaneously heating the multiplicity of containers; (c) measuring the fluorescence in each of the containers; (d) generating thermal unfolding information for the target molecule as a function of temperature for each of the containers; (e) comparing the thermal unfolding information obtained for each of the containers to the thermal unfolding information for the target molecule in the absence of any of the molecules in the multiplicity of different molecules; and (f) ranking the affinities of each of the molecules according to the difference in the thermal unfolding information between the target molecule in each of the containers and the target molecule in the absence of any of the molecules in the multiplicity of different molecules.

    摘要翻译： 本发明提供了一种用于对能够由于热变化而展开的靶分子的多种不同分子的亲和力进行排序的方法。 该方法包括（a）在5-（4“ - 二甲基氨基苯基-2-（4'-苯基）恶​​唑衍生物染料存在下，将目标分子与多个不同分子的一个分子接触， 容器的多样性;（b）同时加热多个容器;（c）测量每个容器中的荧光;（d）作为每个容器的温度的函数产生靶分子的热展开信息;（e ）在不存在多个不同分子中的任何分子的情况下将每个容器获得的热解折叠信息与目标分子的热解折叠信息进行比较;以及（f）根据 在不同分子中多个分子中没有任何分子的情况下，每个容器中的目标分子与靶分子之间的热展开信息的差异。

    Methods of using analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamate 89, asparate 101 or leucine 137
    10.
    发明授权
    Methods of using analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamate 89, asparate 101 or leucine 137 有权
    使用在一种或多种位置谷氨酸盐89，天冬氨酸101或亮氨酸137突变的人碱性成纤维细胞生长因子类似物的方法

    公开(公告)号：US07138374B2

    公开(公告)日：2006-11-21

    申请号：US10785436

    申请日：2004-02-23

    申请人： Barry A. Springer ,  Michael W. Pantoliano ,  Celia M. Sharp

    发明人： Barry A. Springer ,  Michael W. Pantoliano ,  Celia M. Sharp

    IPC分类号： A61K38/18 ,  C07K14/50

    CPC分类号： C07K14/503 ,  A61K38/00

    摘要： The present invention relates to novel muteins of human basic fibroblast growth factor with superagonist properties. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for stimulating cell division, treating a wound, treating ischemia, treating heart disease, treating neural injury, treating peripheral vascular disease, treating a gastric ulcer and treating a duodenal ulcer.

    摘要翻译： 本发明涉及具有超强调性质的人碱性成纤维细胞生长因子的新颖突变蛋白。 公开了蛋白质和各自编码的核酸物质。 本发明还体现了用于扩增所述核酸序列和产生所述突变蛋白的载体和宿主细胞。 还公开了刺激细胞分裂，治疗伤口，治疗缺血，治疗心脏病，治疗神经损伤，治疗外周血管疾病，治疗胃溃疡和治疗十二指肠溃疡的方法。