公开(公告)号：US07544512B2

公开(公告)日：2009-06-09

申请号：US10761717

申请日：2004-01-20

Applicant: Yukun Sun ,  Dengxi Wu ,  Aizhen Wu ,  Zhiyong Zhu ,  Gang Yu ,  Jiaxiang Zhou ,  Shaoling Zhao

Inventor： Yukun Sun ,  Dengxi Wu ,  Aizhen Wu ,  Zhiyong Zhu ,  Gang Yu ,  Jiaxiang Zhou ,  Shaoling Zhao

IPC: A61K38/26 ,  C12N15/16 ,  A61K38/00

CPC classification number: C07K14/605 ,  C07K2319/00

Abstract: The present invention discloses a method of producing polypeptides, including insulinotropic GLP-1 (7-36) polypeptide and/or GLP-1 analogs, by ligating genes in a tandem way. Also disclosed are the recombinant polypeptides produced by such a method. Using the method of this invention, 1 to 32 copies of GLP-1 (7-36) and/or GLP-1 analog genes may be expressed in tandem and the desired polypeptide can be obtained after cleavage of a fusion protein and further processes of separation and purification thus making possible the production of recombinant polypeptides, including recombinant GLP-1 (7-36) and/or GLP-1 analogs on a large scale, at a significantly reduced production cost.

Abstract translation: 本发明公开了通过以串联方式连接基因来产生包括促胰岛素GLP-1（7-36）多肽和/或GLP-1类似物的多肽的方法。 还公开了通过这种方法产生的重组多肽。 使用本发明的方法，可以串联表达1至32拷贝的GLP-1（7-36）和/或GLP-1类似物，并且可以在切割融合蛋白后获得所需的多肽， 分离和纯化，从而以显着降低的生产成本使得大规模生产重组多肽（包括重组GLP-1（7-36）和/或GLP-1类似物）成为可能。