公开(公告)号：US20100184147A1

公开(公告)日：2010-07-22

申请号：US12702428

申请日：2010-02-09

申请人： ALAN T.Y. CHENG ,  Ying Zhou ,  Amitabh Gupta ,  Balazs Hunek ,  Nigel Grinter

发明人： ALAN T.Y. CHENG ,  Ying Zhou ,  Amitabh Gupta ,  Balazs Hunek ,  Nigel Grinter

IPC分类号： C12P21/06 ,  C12N5/02

CPC分类号： C12N5/0018 ,  C12N1/38 ,  C12N2500/02 ,  C12N2500/60

摘要： Methods for controlling the level of dissolved carbon dioxide and limiting osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield are provided. Such control of the level of dissolved carbon dioxide and pH as well as the resulting ability to limit osmolality in a mammalian cell culture process is achieved by adopting alternative pH control strategies and CO2 stripping techniques during a mammalian cell culture process. Such pH control techniques and carbon dioxide stripping occur without foam and with little or no damage to the mammalian cells.

摘要翻译： 提供了用于在哺乳动物细胞培养过程中控制溶解二氧化碳水平和限制重量克分子渗透浓度以增强细胞生长，活力和密度以及提高生物产物浓度和产量的方法。 通过在哺乳动物细胞培养过程中采用替代的pH控制策略和CO 2汽提技术来实现对哺乳动物细胞培养过程中溶解的二氧化碳和pH的水平的控制以及所产生的限制重量克分子渗透浓度的能力。 这种pH控制技术和二氧化碳汽提不发生泡沫，对哺乳动物细胞几乎没有或没有损害。

公开(公告)号：US20100035342A1

公开(公告)日：2010-02-11

申请号：US12536577

申请日：2009-08-06

申请人： ALAN T.Y. CHENG ,  YING ZHOU ,  AMITABH GUPTA ,  BALAZS HUNEK ,  NIGEL GRINTER

发明人： ALAN T.Y. CHENG ,  YING ZHOU ,  AMITABH GUPTA ,  BALAZS HUNEK ,  NIGEL GRINTER

IPC分类号： C12N5/02

CPC分类号： C12N1/38 ,  C12M27/02 ,  C12M29/22

摘要： Methods for controlling the level of dissolved carbon dioxide and limiting osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield are provided. Such control of the level of dissolved carbon dioxide and pH as well as the resulting ability to limit osmolality in a mammalian cell culture process is achieved by adopting alternative pH control strategies and CO2 stripping techniques during a mammalian cell culture process. Such pH control techniques and carbon dioxide stripping occur with little or no damage to the mammalian cells.

摘要翻译： 提供了用于在哺乳动物细胞培养过程中控制溶解二氧化碳水平和限制重量克分子渗透浓度以增强细胞生长，活力和密度以及提高生物产物浓度和产量的方法。 通过在哺乳动物细胞培养过程中采用替代的pH控制策略和CO 2汽提技术来实现对哺乳动物细胞培养过程中溶解的二氧化碳和pH的水平的控制以及所产生的限制重量克分子渗透浓度的能力。 这种pH控制技术和二氧化碳汽提产生对哺乳动物细胞几乎没有或没有损伤。

公开(公告)号：US20080141715A1

公开(公告)日：2008-06-19

申请号：US11639569

申请日：2006-12-15

申请人： Vijayaraghavan Srinivasan Chakravarthy ,  Amitabh Gupta ,  Alan T.Y. Cheng

发明人： Vijayaraghavan Srinivasan Chakravarthy ,  Amitabh Gupta ,  Alan T.Y. Cheng

IPC分类号： F25J3/00

CPC分类号： A61L2/022 ,  F17C2221/014 ,  F17C2260/044

摘要： A system and method for sterilizing a cryogen is provided. In the disclosed embodiment a liquid cryogen is first vaporized and subsequently compressed prior to undergoing sterilization using biological filters. The compressed, sterilized cryogen vapor is then pre-cooled, preferably with the cryogen being vaporized, to produce a partially condensed, cool, sterile cryogen stream and then further condensed using the liquid cryogen to produce a sterilized liquid cryogen.

摘要翻译： 提供了一种用于对冷冻剂灭菌的系统和方法。 在所公开的实施方案中，液体冷冻剂首先在使用生物过滤器进行灭菌之前蒸发并随后压缩。 然后将经压缩的灭菌的冷冻剂蒸气预先冷却，优选在冷冻剂被蒸发的情况下，产生部分冷凝的，冷却的无菌制冷剂流，然后使用液体冷冻剂进一步冷凝以产生无菌液体冷冻剂。

公开(公告)号：US20100035343A1

公开(公告)日：2010-02-11

申请号：US12536559

申请日：2009-08-06

申请人： Alan T.Y. Cheng ,  Ying Zhou ,  Balazs Hunek ,  Amitabh Gupta ,  Nigel Grinter

发明人： Alan T.Y. Cheng ,  Ying Zhou ,  Balazs Hunek ,  Amitabh Gupta ,  Nigel Grinter

IPC分类号： C12N5/02 ,  C12M1/02 ,  C12M1/34 ,  C12M1/36 ,  C12M1/00

CPC分类号： C12N1/38 ,  C12M27/02 ,  C12M29/22

摘要： A system and method for controlling a mammalian cell culture process are provided. Such control of the cell culture process involves control of the level of dissolved carbon dioxide in the cell culture media and the resulting ability to prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide with little or no damage to the mammalian cells. The disclosed methods and systems of dissolved carbon dioxide stripping include enhanced surface gas exchange mechanisms within the bioreactor vessel through the use of an upward flow impeller combined with vertical baffles to convert swirling motions of the liquid into a largely vertical flow.

摘要翻译： 提供了一种用于控制哺乳动物细胞培养方法的系统和方法。 细胞培养过程的这种控制涉及控制细胞培养基中溶解的二氧化碳的水平，并且通过增强对二氧化碳的去除而对哺乳动物细胞几乎没有或没有损伤来实现所产生的渗透压浓度增加的能力。 所公开的溶解二氧化碳汽提方法和系统包括通过使用与垂直挡板结合的向上流动叶轮将生物反应器容器内的表面气体交换机制改进为将液体的旋转运动转化成大体垂直的流动。