公开(公告)号：US11235075B2

公开(公告)日：2022-02-01

申请号：US14787039

申请日：2014-04-28

申请人： THE JOHNS HOPKINS UNIVERSITY ,  KENNEDY KRIEGER INSTITUTE, INC. ,  NATIONAL INSTITUTES OF HEALTH

发明人： Michael T. McMahon ,  Kannie Wai-Yan Chan ,  Michael Christopher Giano ,  Nikita Oskolkov ,  Joel Patrick Schneider ,  Xiaolei Song

IPC分类号： A61B5/055 ,  A61K49/18 ,  A61K49/14

摘要： The present invention provides novel hydrogels through peptides, which are designed to self-assemble and produce magnetic resonance (MR) contrast through chemical exchange saturation transfer (CEST). The location and integrity of these gels could consequently be tracked using MR imaging. The self-assembly of the peptides into hydrogels can be brought about by a change in pH, ionic strength, temperature, and concentration of ions.

公开(公告)号：US20210238233A1

公开(公告)日：2021-08-05

申请号：US17050475

申请日：2019-04-26

申请人： University of Massachusetts ,  The Johns Hopkins University ,  Kennedy Krieger Institute, Inc.

发明人： Miguel Sena Esteves ,  Sourav Roy Choudhury ,  Kathryn Rae Wagner ,  Jennifer Gifford Green ,  Ana Rita Batista

IPC分类号： C07K14/01 ,  C12N7/00

摘要： The disclosure relates, in some aspects, to adeno-associated vims capsid proteins isolated from an in vivo library and recombinant adeno-associated viruses (rAAVs) comprising the same. In some aspects, the disclosure relates to isolated nucleic acids encoding AAV capsid proteins isolated from an in vivo library. In some embodiments, rAAVs and compositions described by the disclosure are useful for delivery of one or more transgenes to the muscle-tissue of a subject.

公开(公告)号：US10544461B2

公开(公告)日：2020-01-28

申请号：US14784720

申请日：2014-04-16

申请人： THE JOHNS HOPKINS UNIVERSITY ,  KENNEDY KRIEGER INSTITUTE ,  DUKE UNIVERSITY

发明人： Jonathan Pevsner ,  Anne Comi ,  Douglas Marchuk ,  Matthew Shirley

IPC分类号： C12Q1/68 ,  C12Q1/6883

摘要： The present invention relates to the fields of neurological and skin disorders. More specifically, the present invention provides methods and compositions for diagnosing and prognosing Sturge-Weber Syndrome (SWS), Klippel-Trenaunay-Weber Syndrome (KTWS), and Port Wine Stains (PWS). In one embodiment, a method for prognosing or monitoring treatment of a patient with SWS, KTWS and/or PWS comprises the steps of (a) providing a sample from the patient undergoing treatment; (b) determining the number of alleles in the sample comprising at least one activating somatic mutation in the guanine nucleotide-binding protein G subunit alpha (GNAQ) gene or protein; (c) comparing the number of alleles comprising the at least one somatic mutation to the number of alleles comprising the somatic mutation from a patient sample provided prior to undergoing treatment; and (d) determining that the patient is improving if there is a decrease in the number of alleles comprising the at least one somatic mutation from the sample of step (a).

公开(公告)号：US10365343B2

公开(公告)日：2019-07-30

申请号：US14895620

申请日：2014-06-03

申请人： THE JOHNS HOPKINS UNIVERSITY ,  KENNEDY KRIEGER INSTITUTE, INC.

发明人： Michael T. McMahon ,  Xiaolei Song ,  Jiadi Xu

IPC分类号： G01V3/00 ,  G01R33/561 ,  A61B5/055 ,  A61B5/00 ,  G01R33/56

摘要： A novel approach for CEST MR imaging is called Multi-echo Length and Offset VARied Saturation (Me-LOVARS) CEST. This method allows efficient collection of additional CEST data without penalty in scan time, which could be useful for enhancing the contrast, increasing the specificity or improving quantification of exchange. As CEST-MRI has shown promise at both the pre-clinical and clinical levels, including for detecting and grading brain tumors and evaluating ischemia, using either endogenous CEST contrast or through administration of CEST contrast agents. This fast and robust imaging method is suitable for accelerating image collection and widening the scope of applications for CEST-MRI.

公开(公告)号：US20230067741A1

公开(公告)日：2023-03-02

申请号：US17930166

申请日：2022-09-07

申请人： University of Massachusetts ,  The Johns Hopkins University ,  Kennedy Krieger Institute, Inc.

发明人： Miguel Sena Esteves ,  Sourav Roy Choudhury ,  Kathryn Rae Wagner ,  Jennifer Gifford Green ,  Ana Rita Batista

IPC分类号： C07K14/01 ,  C12N7/00

摘要： The disclosure relates, in some aspects, to adeno-associated virus capsid proteins isolated from an in vivo library and recombinant adeno-associated viruses (rAAVs) comprising the same. In some aspects, the disclosure relates to isolated nucleic acids encoding AAV capsid proteins isolated from an in vivo library. In some embodiments, rAAVs and compositions described by the disclosure are useful for delivery of one or more transgenes to the muscle-tissue of a subject.

公开(公告)号：US09696309B2

公开(公告)日：2017-07-04

申请号：US14408393

申请日：2012-12-07

申请人： THE JOHNS HOPKINS UNIVERSITY ,  KENNEDY KRIEGER INSTITUTE, INC.

发明人： Irina Burd ,  Talaibek Borbiev ,  Elisabeth Nigrini ,  Michael Johnston

IPC分类号： G01N33/535 ,  G01N33/573 ,  G01N33/68

CPC分类号： G01N33/573 ,  G01N33/535 ,  G01N33/6896 ,  G01N2333/90254 ,  G01N2440/14 ,  G01N2800/28

摘要： The present invention relates to the field of biomarkers. More specifically, the present invention relates to biomarkers useful in diagnosing brain injuries. In a specific embodiment, a method for diagnosing perinatal brain injury in a patient comprises the steps of (a) determining the ratio of phosphorylated nNOS to unphosphorylated nNOS in a sample collected from the patient using an ELISA; and (b) comparing the ratio with predefined ratios of the same proteins that correlate to a patient having perinatal brain injury and predefined ratios of the same proteins that correlate to a patient not having perinatal brain injury, wherein a correlation to one of the predefined ratios provides the diagnosis.

公开(公告)号：US20100221250A1

公开(公告)日：2010-09-02

申请号：US12690045

申请日：2010-01-19

申请人： Kyung Jin Kim ,  John Laterra ,  Bachchu Lal

发明人： Kyung Jin Kim ,  John Laterra ,  Bachchu Lal

IPC分类号： A61K39/395 ,  A61P35/00

CPC分类号： C07K16/22 ,  A61K2039/505 ,  C07K16/3053 ,  C07K2317/73 ,  C07K2317/76

摘要： The application is directed toward a method of treating a brain tumor in a patient comprising systemically administering a monoclonal antibody.

摘要翻译： 本申请涉及治疗患者的脑肿瘤的方法，其包括全身施用单克隆抗体。

公开(公告)号：US12077562B2

公开(公告)日：2024-09-03

申请号：US17930166

申请日：2022-09-07

申请人： University of Massachusetts ,  The Johns Hopkins University ,  Kennedy Krieger Institute, Inc.

发明人： Miguel Sena Esteves ,  Sourav Roy Choudhury ,  Kathryn Rae Wagner ,  Jennifer Gifford Green ,  Ana Rita Batista

IPC分类号： C07K14/01 ,  C07K14/005 ,  C07K14/015 ,  C07K14/47 ,  C12N7/00 ,  A61K38/00

CPC分类号： C07K14/01 ,  C12N7/00 ,  A61K38/00 ,  C12N2310/141 ,  C12N2750/14122

摘要： The disclosure relates, in some aspects, to adeno-associated virus capsid proteins isolated from an in vivo library and recombinant adeno-associated viruses (rAAVs) comprising the same. In some aspects, the disclosure relates to isolated nucleic acids encoding AAV capsid proteins isolated from an in vivo library. In some embodiments, rAAVs and compositions described by the disclosure are useful for delivery of one or more transgenes to the muscle-tissue of a subject.

公开(公告)号：US20230408611A1

公开(公告)日：2023-12-21

申请号：US18037726

申请日：2021-11-19

申请人： The Johns Hopkins University ,  Kennedy Krieger Institute, Inc.

发明人： Peter Van Zijl ,  Yang Zhou ,  Nirbhay N. Yadav

IPC分类号： G01R33/48

CPC分类号： G01R33/4828

摘要： Some embodiments provide a method for magnetic resonance imaging of polysaccharide molecules, that includes providing a magnetic field that is sufficiently homogeneous over an imaging volume, generating a spatial encoding in the magnetic field, and acquiring one or more water proton signal intensity measurements at each of multiple voxels within the imaging volume. The signal intensity measurements are acquired at one or more irradiation frequencies at lower parts-per-million (ppm) than a baseline frequency associated with free water protons. The method includes generating, based on the water proton signal intensity measurements in each voxel, a water proton signal intensity map of the relayed Nuclear Overhauser Effect (rNOE) exchange process of aliphatic protons in the polysaccharide molecules to free water protons in the imaging volume, and generating, using a calibration of the water proton signal intensity measurements for the rNOE exchange process, a concentration map of the polysaccharide molecules in the imaging volume.

公开(公告)号：US20230220067A1

公开(公告)日：2023-07-13

申请号：US17928996

申请日：2021-06-03

申请人： The Johns Hopkins University ,  UNIVERSITY OF MARYLAND, BALTIMORE ,  THE KENNEDY KRIEGER INSTITUTE, INC.

发明人： John J. Laterra ,  Amy M. Fulton

IPC分类号： C07K16/28 ,  A61P35/00 ,  C07K16/30

CPC分类号： C07K16/28 ,  A61P35/00 ,  C07K16/30 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/732 ,  A61K2039/505

摘要： Provided herein are humanized monoclonal antibodies that bind to potassium two pore domain channel subfamily K member 9 (KCNK9). The humanized monoclonal antibodies comprise (a) a heavy chain variable region comprising an amino acid sequence of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, or SEQ ID NO: 13; and (b) a light chain variable region comprising an amino acid sequence of SEQ ID NO: 14, SEQ ID NO: 15, or SEQ ID NO: 16. Methods of using the antibodies to inhibit the growth of KCNK9-expressing cells also are provided.